Gene of enzyme having activity to generate lachrymatory factor

Abstract

It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.

1 - 4 . (canceled) 
     
     
         5 . A DNA which codes for isolated isozyme of a lachrymatory factor producing enzyme which converts lachrymatory factor precursors to lachrymatory factor. 
     
     
         6 . The DNA according to  claim 5 , which comprises SEQ ID No. 4. 
     
     
         7 . The DNA according to  claim 5 , wherein the DNA containing the base sequence that codes for proteins or polypeptides is DNA indicated by SEQ ID No. 5. 
     
     
         8 . A recombinant vector comprising the DNA of  claim 5 ,  6  or  7 . 
     
     
         9 . A host cell transformed with the recombinant vector defined in  claim 8 . 
     
     
         10 . (canceled) 
     
     
         11 . A method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity, comprising culturing host cells transformed by the recombinant vector of  claim 8 , and then isolating a protein or polypeptide with lachrymatory factor producing enzyme activity that is produced in a culture medium or cells. 
     
     
         12 . Anti-sense RNA which has a base sequence complementary to that of the mRNA corresponding to the DNA defined in  claim 5 ,  6  or  7 . 
     
     
         13 - 18 . (canceled) 
     
     
         19 . The DNA of  claim 5 , which encodes a protein which comprises an N-terminal amino acid sequence selected from the group consisting of amino acids 1 to 5 of SEQ ID NO:1, amino acids 1 to 5 of SEQ ID NO:2, and amino acids 1 to 10 of SEQ ID NO:3. 
     
     
         20 . The DNA of  claim 5 , which encodes a protein comprising SEQ ID No.1. 
     
     
         21 . The DNA of  claim 5 , which encodes a protein comprising SEQ ID No. 2. 
     
     
         22 . The DNA of  claim 5 , which encodes a protein comprising SEQ ID No. 3. 
     
     
         23 . A recombinant vector comprising the DNA of  claim 19 ,  20 ,  21 , or  22 . 
     
     
         24 . A host cell transformed with the recombinant vector of  claim 23 . 
     
     
         25 . A method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity, comprising culturing host cells transformed by the recombinant vector of  claim 23 , and then isolating a protein or polypeptide with lachrymatory factor producing enzyme activity that is produced in a culture medium or cells. 
     
     
         26 . Anti-sense RNA which has a base sequence complementary to that of the mRNA corresponding to the DNA of  claim 19 ,  20 ,  21 , or  22 .