Sample preparation device

Abstract

A sample preparation device is disclosed. The sample preparation device includes a housing defining a passage way between a first opening and a second opening; and a sample filter occupying a section of said passage way. The sample filter contains a monolith adsorbent that specifically binds to nucleic acids. Also disclosed are sample filters containing glass frit is coated with an capture agent that binds specifically to an analyte of interest, sample filters containing a hydrophilic matrix with impregnated chemicals that lyses cell membranes, a cartridge base and an integrated sample preparation cartridge.

1 . A sample preparation device comprising:
 a housing defining a passage way between a first opening and a second opening; and   a sample filter occupying a section of said passage way, said sample filter comprising a monolith adsorbent that specifically binds to nucleic acids.   
     
     
         2 . The sample preparation device of  claim 1 , wherein said sample filter is chemically treated to create positive charges. 
     
     
         3 . The sample preparation device of  claim 1 , wherein said monolith adsorbent is selected from the group consisting of glass frit, porous glass monolith, and porous monolithic polymers. 
     
     
         4 . The sample preparation device of  claim 3 , wherein said monolith adsorbent comprises a glass frit. 
     
     
         5 . The sample preparation device of  claim 3 , wherein said monolith adsorbent comprises porous glass monolith. 
     
     
         6 . The sample preparation device of  claim 3 , wherein said monolith adsorbent comprises porous monolithic polymers. 
     
     
         7 . The sample preparation device of  claim 1 , wherein said sample filter comprises a first section that binds specifically to nucleic acids and a second section that binds specifically to an analyte of interest. 
     
     
         8 . The sample preparation device of  claim 7 , wherein said analyte of interest is a protein. 
     
     
         9 . The sample preparation device of  claim 1 , wherein said first opening has a diameter that is greater than the diameter of said second opening, and wherein said sample filter is placed in the close proximity of said second opening. 
     
     
         10 . The sample preparation device of  claim 9 , wherein the side of said frit is tapered to the contour of said passage way. 
     
     
         11 . The sample preparation device of  claim 1 , wherein said housing further comprises a second sample filter placed in the close proximity of said first sample filter. 
     
     
         12 . The sample preparation device of  claim 1 , wherein said housing further comprises an aerosol filter placed in the proximity of said first opening. 
     
     
         13 . The sample preparation device of  claim 1 , wherein said housing is in the configuration of a pipette tip. 
     
     
         14 . An integrated sample preparation cartridge, comprising:
 a sample preparation device comprising:
 a housing defining a passage way between a first opening and a second opening; and 
 a sample filter occupying a section of the passage way, said sample filter comprising an adsorbent that specifically binds to an analyte of interest; and 
   a cartridge base comprising:
 a first port configured to interface with the sample preparation device; 
 a second port configured to interface with a liquid delivery device; and 
 a first channel connecting said first port to said second port. 
   
     
     
         15 . The integrated sample preparation cartridge of  claim 14 , wherein said cartridge base is made of a material selected form the group consisting of polycarbonate, polystyrene and polypropylene. 
     
     
         16 . The integrated sample preparation cartridge of  claim 14 , wherein said liquid delivery device is a flow control station. 
     
     
         17 . The integrated sample preparation cartridge of  claim 14 , wherein said second port comprises a Luer-activated valve. 
     
     
         18 . The integrated sample preparation cartridge of  claim 14 , wherein said cartridge base further comprising:
 a third port; and   a second channel connecting said third port to said first port.   
     
     
         19 . The integrated sample preparation cartridge of  claim 18 , wherein said third port is a self-sealing port. 
     
     
         20 . The integrated sample preparation cartridge of  claim 18 , wherein said third port comprises a septum or a one-way valve. 
     
     
         21 . A cartridge base, comprising:
 a base body having a top side and a bottom side;   a first port, formed on said top side of the base body and configured to interface with a sample preparation device;   a second port, formed on said bottom side of the base body and configured to interface with a liquid delivery device; and   a first channel connecting said first port to said second port.   
     
     
         22 . The cartridge base of  claim 21 , further comprising
 one or more additional ports; and   one or more additional channels connecting said one or more additional ports to said first port.   
     
     
         23 . A sample preparation device comprising:
 a housing defining a passage way between a first opening and a second opening; and   a glass frit occupying a section of the passage way, said glass frit is coated with an capture agent that binds specifically to an analyte of interest.   
     
     
         24 . The sample preparation device of  claim 23 , wherein said capture agent is an antibody. 
     
     
         25 . The sample preparation device of  claim 23 , wherein said capture agent is a lectin. 
     
     
         26 . A sample preparation device comprising:
 a housing defining a passage way between a first opening and a second opening; and   a filter occupying a section of the passage way, said filter comprises a hydrophilic matrix with impregnated chemicals that lyses cell membranes.   
     
     
         27 . The sample preparation device of  claim 26 , wherein said hydrophilic matrix is FTA paper®. 
     
     
         28 . A method for purifying an analyte from a liquid sample, comprising:
 placing said liquid sample in a container;   withdrawing at least a portion of said liquid sample into a sample preparation device comprising:
 a housing defining a passage way between a first opening and a second opening; and 
 a filter occupying a section of said passage way, said sample filter comprising a material that specifically binds to said analyte, 
 wherein said portion of liquid sample is drawn into said housing via the first opening and passing through said filter, and wherein said analyte binds to said filter while passing through said filter; 
   expelling said portion of liquid sample from said sample preparation device via the first opening, wherein said portion of liquid sample passes through said filter a second time while exiting said sample preparation device; and   eluting said analyte from said filter by withdrawing an eluting buffer into said sample preparation device via the first opening and expelling said eluting buffer from said sample preparation device via the first opening, wherein said eluting buffer passes through said filter while entering and exiting said sample preparation device.   
     
     
         29 . The method of  claim 28 , further comprising:
 washing said filter by withdrawing a washing buffer into said sample preparation device via the first opening and expelling said washing buffer from said sample preparation device via the first opening, wherein said washing buffer passes through said filter while entering and exiting said sample preparation device.   
     
     
         30 . The method of  claim 29  wherein said washing step is repeated two or more times. 
     
     
         31 . The method of  claim 28 , wherein said sample withdrawing and sample expelling steps are repeated until all said liquid sample passes through said filter at least once. 
     
     
         32 . The method of  claim 28 , wherein said analyte is a nucleic acid and said filter comprises a glass frit. 
     
     
         33 . The method of  claim 28 , wherein said sample preparation device has the configuration of a pipette tip. 
     
     
         34 . The method of  claim 28 , wherein said withdrawing, expelling and eluting steps are controlled by an electronic pipettor. 
     
     
         35 . The method of  claim 28 , wherein said withdrawing, expelling and eluting steps are controlled by an robotic pipetting station. 
     
     
         36 . A method for purifying an analyte from a sample, comprising:
 passing said sample through a sample preparation device comprising:
 a housing defining a passage way between a first opening and a second opening; and 
 a filter occupying a section of said passage way, 
 wherein said sample filter comprising a material that specifically binds to said analyte and said sample passes through said sample filter while passing through said sample preparation device; and 
   eluting analyte bound to said sample filter with an eluting solution,   wherein said sample and said eluting solution enter and exit said housing through the same opening.   
     
     
         37 . The method of  claim 36 , wherein said sample preparation device is attached to a cartridge base comprising a first port configured to receive said sample preparation device and a second port configured to receive a liquid delivery device. 
     
     
         38 . The method of  claim 36 , further comprising:
 washing said filter with a washing solution, wherein said washing solution enter and exit said housing through the same opening.   
     
     
         39 . A sample purification system, comprising:
 a sample preparation device comprising:
 a housing defining a passage way between a first opening and a second opening; and 
 a sample filter occupying a section of said passage way, said sample filter comprising a monolith adsorbent that specifically binds to an analyte of interest; 
   a cartridge base comprising:
 a first port configured to receive said sample preparation device; 
 a second port configured to receive a liquid delivery device; 
 a channel connecting said first sample port with said second sample port; and 
   a liquid delivery device, connected to said cartridge base, that controls fluid flow in said sample preparation device and said cartridge base.   
     
     
         40 . The sample purification system of  claim 39 , wherein said liquid delivery device delivers liquids into and removes liquids from said sample preparation device through the same opening on said housing.