US2009113565A1PendingUtilityA1

Methods for Detecting Fluorescent Proteins and Animals Having Fluorescent Proteins

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Assignee: GIBBS PATRICK D LPriority: Dec 9, 2002Filed: Jul 6, 2007Published: Apr 30, 2009
Est. expiryDec 9, 2022(expired)· nominal 20-yr term from priority
Inventors:Patrick D. L. GibbsRobert W. CarterMichael C. Schmale
C12N 15/8509A01K 67/0275A01K 2227/40C07K 14/43595C12N 13/00C12N 5/16C07K 14/47
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PatentIndex Score
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Claims

Abstract

Provided are methods for detecting expression of nucleic acids encoding mutant fluorescent proteins. In the methods, a nucleic acid encoding a mutant fluorescent protein, or a full complement thereof, is introduced into a cell or organism. The cell or organism is allowed to replicate. Expression of the nucleic acid is detected by emission of fluorescent light. Animals including a nucleic acid encoding a mutant fluorescent protein, or a full complement thereof, are also provided.

Claims

exact text as granted — not AI-modified
1 . A method comprising:
 introducing a nucleic acid encoding a mutant red fluorescent protein, or a full complement thereof, into a cell or organism;   allowing the cell or organism to replicate; and   detecting expression of the nucleic acid by emission of fluorescent light.   
     
     
         2 . The method of  claim 1 , wherein the nucleic acid is introduced into a eukaryotic cell. 
     
     
         3 . The method of  claim 1 , wherein the nucleic acid is introduced into a prokaryotic cell. 
     
     
         4 . The method of  claim 1 , wherein the expression of the nucleic acid expression is detected in vivo. 
     
     
         5 . The method of  claim 1 , wherein the expression of the nucleic acid is detected in vitro. 
     
     
         6 . The method of  claim 1 , wherein the nucleic acid comprises SEQ ID NO: 1 (Red I) or a full cDNA of SEQ ID NO: 1. 
     
     
         7 . The method of  claim 1 , wherein the nucleic acid comprises SEQ ID NO: 3 (Red II) or a full cDNA of SEQ ID NO: 3. 
     
     
         8 . An animal comprising a nucleic acid encoding a mutant red fluorescent protein, or a full complement thereof. 
     
     
         9 . The animal of  claim 8 , wherein the animal is a zebrafish. 
     
     
         10 . The animal of  claim 8 , wherein the nucleic acid comprises SEQ ID NO: 1 (Red I) or a full cDNA of SEQ ID NO:1. 
     
     
         11 . The animal of  claim 8 , wherein the nucleic acid comprises SEQ ID NO: 3 (Red II) or a full cDNA of SEQ ID NO: 3. 
     
     
         12 . A method comprising
 introducing a nucleic acid encoding a mutant green fluorescent protein, or a full complement thereof, into a cell or organism;   allowing the cell or organism to replicate; and   detecting expression of the nucleic acid by emission of fluorescent light.   
     
     
         13 . The method of  claim 12 , wherein the nucleic acid is introduced into a eukaryotic cell. 
     
     
         14 . The method of  claim 12 , wherein the nucleic acid is introduced into a prokaryotic cell. 
     
     
         15 . The method of  claim 12 , wherein the expression of the nucleic acid is detected in vivo. 
     
     
         16 . The method of  claim 12 , wherein the expression of the nucleic acid is detected in vitro. 
     
     
         17 . The method of  claim 12 , wherein the nucleic acid comprises SEQ ID NO: 5 (Green I) or a full cDNA of SEQ ID NO:5. 
     
     
         18 . The method of  claim 12 , wherein the nucleic acid comprises SEQ ID NO: 7 (Green II) or a full cDNA of SEQ ID NO:7. 
     
     
         19 . An animal comprising a nucleic acid encoding a mutant green fluorescent protein, or a full complement thereof. 
     
     
         20 . The animal of  claim 19 , wherein the animal is a zebrafish. 
     
     
         21 . The method of  claim 19 , wherein the nucleic acid comprises SEQ ID NO: 5 (Green I) or a full cDNA of SEQ ID NO:5. 
     
     
         22 . The animal of  claim 19 , wherein the nucleic acid comprises SEQ ID NO: 7 (Green II) or a full cDNA of SEQ ID NO:7. 
     
     
         23 . A transgenic cell comprising an isolated nucleic acid comprising SEQ ID NO: 1 (Red I) or a full cDNA of SEQ ID NO:1. 
     
     
         24 . The cell of  claim 23 , wherein the nucleic acid is integrated into a genome of the cell. 
     
     
         25 . The cell of  claim 23 , wherein the nucleic acid is not integrated into a genome of the cell. 
     
     
         26 . A transgenic cell comprising an isolated nucleic acid comprising SEQ ID NO: 5 (Green I) or a full cDNA of SEQ ID NO: 5. 
     
     
         27 . The cell of  claim 26 , wherein the nucleic acid is integrated into a genome of the cell. 
     
     
         28 . The cell of  claim 26 , wherein the nucleic acid is not integrated into a genome of the cell. 
     
     
         29 . A transgenic cell comprising an isolated nucleic acid comprising SEQ ID NO: 3 (Red II) or a full cDNA of SEQ ID NO: 3. 
     
     
         30 . The cell of  claim 29 , wherein the nucleic acid is integrated into a genome of the cell. 
     
     
         31 . The cell of  claim 29 , wherein the nucleic acid is not integrated into a genome of the cell. 
     
     
         32 . A transgenic cell comprising An isolated nucleic acid comprising SEQ ID NO: 7 (Green II) or a full cDNA of SEQ ID NO: 7. 
     
     
         33 . The cell of  claim 32 , wherein the nucleic acid is integrated into a genome of the cell. 
     
     
         34 . The cell of  claim 32 , wherein the nucleic acid is not integrated into a genome of the cell.

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