Andrographis paniculata extract

Abstract

An extract of Andrographis paniculata extract containing andrographolide, 14-deoxyandrographolide, 14-deoxy-11,12-dehydroandrographolide, neoandrographolide, polysacchlorides, and flavanoids. Also disclosed is a pharmaceutical composition containing such an extract and its use for treating inflammatory bowel disease.

1 . An extract of Andrographis paniculata, comprising andrographolide lactones, polysaceharides, and flavonoids; wherein the andrographolide lactones, the polysaceharides, and the flavonoids constitute 10-22%, 18-28%, and 10-15% of the dry weight of the extract, respectively; and the andrographolide lactones include andrographolide, 14- deoxyandrographolide, 14-deoxy- 11, 12-dehydrogen-andrographolide, and neoandrographolide, which constitute 2-20%, 0.01-6%, 1-6%, and 1-5% of the dry weight of the extract, respectively. 
   
   
       2 . The extract of  claim 1 , wherein the andrographolide lactones, the polysaccharides, and the flavonoids constitute 13-17%, 20-25%, and 12-14% of the dry weight of the extract, respectively. 
   
   
       3 . The extract of  claim 1 , wherein the andrographolide, the 14-deoxyandrographolide, the 14-deoxy-11, 12-dehydroandrographolide, and the neoandrographolide constitute 3-10%, 0.01-2%, 2-5%, and 2-4% of the dry weight of the extract, respectively. 
   
   
       4 . The extract of  claim 3 , wherein the andrographolide, the 14-deoxyandrographolide, the 14-deoxy-11,12-dehydrogen-andrographolide, and the neoandrographolide constitute 6-10%, 0.01-1%, 2-4%, and 2-4% of the dry weight of the extract, respectively. 
   
   
       5 . The extract of  claim 2 , wherein the andrographolide, the 14-deoxyandrographolide, the 14-deoxy-11, 12-dehydrogen-andrographolide, and the neoandrographolide constitute 3-10%, 0.01-2%, 2-5%, and 2-4% of the dry weight of the extract, respectively. 
   
   
       6 . The extract of  claim 5 , wherein the andrographolide, the 14-deoxyandrographolide, the 14-deoxy-11, 12-dehydrogen-andrographolide, and the neoandrographolide constitute 6-10%, 0.01-1%, 2-4%, and 2-4% of the dry weight of the extract, respectively. 
   
   
       7 . The extract of  claim 1 , wherein the polysaccharides, the flavonoids, the andrographolide, the 14-deoxyandrographolide, the 14-deoxy-11,12-dehydrogen-andrographolide, and the neoandrographolide constitute 24.6%, 12.8%, 9.2%, <0.1%, 2.6%, and 3.0% of the dry weight of the extract, respectively. 
   
   
       8 . An extract prepared from Andrographis paniculata, wherein the extract is prepared by a process comprising:
 mixing an Andrographis paniculata plant with 90-95% ethanol at 75-80° C.,   separating the supernatant from the mixture, and   removing ethanol from the supernatant to provide the extract.   
   
   
       9 . A pharmaceutical composition comprising:
 an extract of Andrographis paniculata containing andrographolide lactones, polysaccharides, and flavonoids; wherein the andrographolide lactones, the polysaccharides, and the flavanoids constitute 10-22%, 18-28%, and 10-15% of the dry weight of the extract, respectively; and the andrographolide lactones include andrographolide, 14-deoxyandrographolide, 14-deoxy- 11 ,12-dehydrogen-andrographolide, and neoandrographolide, which constitute 2-20%, 0.01-6%, 1-6%, and 1-5% of the dry weight of the extract, respectively; and   a pharmaceutically acceptable carrier.   
   
   
       10 . The pharmaceutical composition of  claim 9 , wherein the andrographolide lactones, the polysaccharides, and the flavonoids constitute 13-17%, 20-25%, and 12-14% of the dry weight of the extract, respectively. 
   
   
       11 . The pharmaceutical composition of  claim 9 , wherein the andrographolide, the 14-deoxyandrographolide, the 14-deoxy- 11, 12-dehydrogen-andrographolide, and the neoandrographolide constitute 3-10%, 0.01-2%, 2-5%, and 2-4% of the dry weight of the extract, respectively. 
   
   
       12 . The pharmaceutical composition of  claim 9 , wherein the polysaccharides, the flavonoids, the andrographolide, the 14-deoxyandrographolide, the 14-deoxy- 11,12-dehydrogen-andrographolide, and the neoandrographolide constitute 24.6%, 12.8%, 9.2%, <0.1%, 2.6%, and 3.0% of the dry weight of the extract, respectively. 
   
   
       13 - 25 . (canceled)