Device for Containing, Reacting and Measuring, and Method of Containing, Reacting and Measuring

Abstract

The invention relates to a device for containing, reacting and measuring, and a method of containing, reacting and measuring, and provides a device for containing, reacting and measuring, and a method of containing, reacting and measuring which is also able to effectively and quickly perform the reaction processing, measuring and identification. The invention comprises; a transparent container section having a liquid inlet/outlet and which is able to contain a base member with various substances for detection having predetermined chemical structures fixed at respective fixed positions which are arranged in a predetermined condition, and with each of the chemical structures associated with each of the fixed positions, a drawing and discharging section which is able to draw and discharge the liquid into and from the container section via the inlet/outlet, and a measuring device which is able to receive light from the contained base member, external to the container section and in a condition associated with the fixed position.

1 - 14 . (canceled) 
   
   
       15 . A method of containing, reacting and measuring comprising:
 a containing step comprising containing a base member with various substances for detection having predetermined chemical structures fixed thereto along a longitudinal direction with each of the chemical structures associated with their fixed positions in a transparent tubular container section; said tubular container section having a diameter and a longitudinal length;   a reaction step comprising drawing a liquid comprising one or more labeled target substances to inside said tubular container section via an inlet/outlet and immersing said base member in said liquid to react said one or more labeled target substances with said substances for detection at the respective fixed positions along the longitudinal direction, wherein drawing said liquid comprises drawing said liquid by using a drawing and discharging section connected to said tubular container section;   a measurement preparation step comprising removing said liquid and any target substance which has not contributed to the reaction from said tubular container section via the inlet/outlet by using the drawing and discharging section; and   a measurement step comprising:
 effecting relative translational and/or rotational movement between the base member and a light receiving section positioned outside of the tubular container section; 
 irradiating light to the base member during effecting relative translational and/or rotational movement between the base member and the light receiving section, comprising irradiating light to the base member via:
 all around the perimeter of the tubular container section, and 
 along at least a portion of the longitudinal length of the tubular container section; and 
 
 measuring light from the respective fixed positions along the longitudinal direction using the light receiving section during effecting relative translational and/or rotational movement between the base member and the light receiving section, comprising measuring light from the respective fixed positions via:
 all around the perimeter of the tubular container section, and 
 along at least a portion of the longitudinal length of the tubular container section. 
 
   
   
   
       16 . A method of containing, reacting and measuring according to  claim 15 , wherein in said measurement preparation step there is included a step for drawing measurement liquid after removing target substance which have not contributed to reaction and liquid suspending these, and said measurement step measures in a condition with said base member immersed in measurement liquid. 
   
   
       17 . A method of containing, reacting and measuring according to  claim 15 , wherein the reaction step comprises at least one of the following:
 shaking the container section; and   discharging the liquid from the container section via the inlet/outlet by using the drawing and discharging section and then repeating drawing a liquid comprising the labeled target substance to inside the container section.   
   
   
       18 . A method of containing, reacting and measuring comprising:
 a containing step comprising containing a base member with various substances for detection having predetermined chemical structures fixed thereto along a longitudinal direction with each of the chemical structures associated with their fixed positions through a container opening in a transparent tubular container section, said tubular container section having a diameter and a longitudinal length;   a reaction step comprising drawing a liquid comprising a labeled target substance to inside said tubular container section via an inlet/outlet by using a drawing and discharging section connected to the container opening and immersing said base member in said liquid to react said target substance with said substance for detection;   a measurement preparation step comprising removing said liquid and any target substance which has not contributed to the reaction via the inlet/outlet by using the drawing and discharging section; and   a measurement step comprising measuring light from the base member contained in said tubular container section in a condition associated with the fixed position, by scanning all fixed positions of said base member by:   relatively moving said tubular container section or a light receiving position, and   scanning all around the perimeter of the tubular container section and along at least a portion of the longitudinal length of the tubular container section during relatively moving said tubular container section or said light receiving position.   
   
   
       19 . A method of containing, reacting and measuring according to  claim 18 , wherein in said measurement preparation step there is included a step for drawing measurement liquid after removing target substances which have not contributed to reaction and liquid suspending these, and said measurement step measures in a condition with said base member immersed in measurement liquid. 
   
   
       20 . A method of containing, reacting and measuring according to  claim 18 , wherein in said reaction step, said container section is shaken, or drawing and discharging are repeated. 
   
   
       21 . A method of containing, reacting and measuring comprising:
 a containing step comprising containing a base member of a long and slender shape, with various substances for detection having predetermined chemical structures fixed thereto along a longitudinal direction with each of the chemical structures associated with their fixed positions through a container opening in a transparent tubular container section, said tubular container section having a diameter and a longitudinal length;   a reaction step comprising drawing a liquid comprising one or more labeled target substances to inside said tubular container section via an inlet/outlet and immersing said base member in said liquid to react said one or more labeled target substances with said substance for detection at the respective fixed positions along the longitudinal direction, wherein drawing said liquid comprises drawing said liquid by using a drawing and discharging section connected to said tubular container section;   a measurement preparation step comprising removing said liquid and any target substance which has not contributed to the reaction from said tubular container section via the inlet/outlet by using the drawing and discharging section; and   a measurement step, comprising:
 effecting relative translational and/or rotational movement between the base member and a light receiving section positioned outside of the container section; and 
 measuring light from the respective fixed positions along the longitudinal direction using the light receiving section during effecting relative translational and/or rotational movement between the base member and the light receiving section, comprising measuring light from the respective fixed positions via:
 all around the perimeter of the tubular container section, and 
 along at least a portion of the longitudinal length of the tubular container section. 
 
   
   
   
       22 . A method of containing, reacting and measuring according to  claim 21 , wherein in said measurement preparation step there is included a step for drawing measurement liquid after removing target substances which have not contributed to reaction and liquid suspending these, and said measurement step measures in a condition with said base member immersed in measurement liquid. 
   
   
       23 . A method containing, reacting and measuring according to  claim 21 , wherein the reaction step comprises at least one of the following:
 shaking the container section; and   discharging the liquid from the container section via the inlet/outlet by using the drawing, and discharging section and then repeating drawing a liquid comprising the labeled target substance to inside the container section.