Gdc-1 genes conferring herbicide resistance

Abstract

Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding an amino acid sequence shown in SEQ ID NO:3, 6, 8, 11, 19, or 21, or a nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 5, 7, 9, 10, 18, or 20, as well as variants and fragments thereof.

1 . An isolated nucleic acid molecule selected from the group consisting of:
 a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1, 2, 4, 5, 9, 10, 18, or 20;   b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:3, 6, 11, 19, or 21;   c) a nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO:3, 6, 11, 19, or 21, wherein said polypeptide has glyphosate resistance and decarboxylase activity; and   d) a complement of any of a)-c).   
     
     
         2 . The isolated nucleic acid molecule of  claim 1 , wherein said nucleotide sequence is a synthetic sequence that has been designed for expression in a plant. 
     
     
         3 . The nucleic acid molecule of  claim 2 , wherein said synthetic sequence has an increased GC content relative to the GC content of SEQ ID NO: 1, 2, 4, 5, 9, 10, 18, or 20. 
     
     
         4 . A vector comprising the nucleic acid molecule of  claim 1 . 
     
     
         5 . The vector of  claim 4 , further comprising a nucleic acid molecule encoding a heterologous polypeptide. 
     
     
         6 . A host cell that contains the vector of  claim 4 . 
     
     
         7 . The host cell of  claim 6  that is a bacterial host cell. 
     
     
         8 . The host cell of  claim 6  that is a plant cell. 
     
     
         9 . A transgenic plant comprising the host cell of  claim 8 . 
     
     
         10 . The plant of  claim 9 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape. 
     
     
         11 . A transgenic seed comprising the nucleic acid molecule of  claim 1 . 
     
     
         12 . An isolated polypeptide selected from the group consisting of:
 a) a polypeptide comprising the amino acid sequence of SEQ NO:3, 6, 11, 19, or 21;   b) a polypeptide encoded by the nucleotide sequence of SEQ ID NO:1, 2, 4, 5, 9, 10, 18, or 20; and   c) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:3, 6, 11, 19, or 21, wherein said polypeptide has glyphosate resistance and decarboxylase activity.   
     
     
         13 . The polypeptide of  claim 12  further comprising a heterologous amino acid sequence. 
     
     
         14 . A method for producing a polypeptide with glyphosate resistance activity, comprising culturing the host cell of  claim 6  under conditions in which a nucleic acid molecule encoding the polypeptide is expressed. 
     
     
         15 . A method for conferring resistance to glyphosate in a plant, said method comprising transforming said plant with a DNA construct, said construct comprising a promoter that drives expression in a plant cell operably linked with a nucleotide sequence at least 95% identical to the nucleotide sequence of SEQ ID NO:3, 6, 11, 19, or 21, and regenerating a transformed plant, wherein said nucleotide sequence encodes a polypeptide that has glyphosate resistance and decarboxylase activity. 
     
     
         16 . A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having glyphosate resistance activity, wherein said nucleotide sequence is selected from the group consisting of:
 a) the nucleotide sequence of SEQ ID NO:1, 2, 4, 5, 9, 10, 18, or 20;   b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:3, 6, 11, 19, or 21; and   c) a nucleotide sequence encoding a polypeptide having at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO:3, 6, 11, 19, or 21, wherein said polypeptide has glyphosate resistance and decarboxylase activity; wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.   
     
     
         17 . A plant cell having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having herbicide resistance activity, wherein said nucleotide sequence is selected from the group consisting of:
 a) the nucleotide sequence of SEQ ID NO:1, 2, 4, 5, 9, 10, 18, or 20;   b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:3, 6, 11, 19, or 21; and   c) a nucleotide sequence encoding a polypeptide having at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO:3, 6, 11, 19, or 21, wherein said polypeptide has glyphosate resistance and decarboxylase activity; wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.