US2009181423A1PendingUtilityA1

Substantially animal protein-free recombinant furin and methods for producing same

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Assignee: BAXTER INTPriority: Dec 31, 2007Filed: Dec 19, 2008Published: Jul 16, 2009
Est. expiryDec 31, 2027(~1.4 yrs left)· nominal 20-yr term from priority
Inventors:Barbara PlaimauerSimone Von FircksLeopold GrillbergerMeinhard HasslacherRoland GeyerArtur MittererManfred Reiter
A61P 43/00A61P 7/02A61P 7/04C12N 9/6454
56
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Claims

Abstract

The present invention relates to recombinant furin (rFurin) and methods for producing rFurin. More specifically, the invention relates to substantially animal protein-free rFurin and methods for producing substantially animal protein-free rFurin.

Claims

exact text as granted — not AI-modified
1 . A composition comprising substantially animal protein-free recombinant furin. 
   
   
       2 . The composition of  claim 1  comprising recombinant furin at an activity of at least about 10000 U furin/mL and CHO host cell protein at a concentration less than about 11 μg protein/mL. 
   
   
       3 . The composition of  claim 1  comprising recombinant furin at an activity of at least about 10000 U furin/mL and CHO host cell protein at a concentration less than about 1.0 ng protein/U furin activity. 
   
   
       4 . The composition of  claim 1  comprising recombinant furin at an activity of at least about 10000 U furin/mL and CHO host cell DNA at a concentration less than about 14 ng DNA/mL. 
   
   
       5 . The composition of  claim 1  comprising recombinant furin at an activity of at least about 10000 U furin/mL and CHO host cell DNA at a concentration less than about 0.5 pg DNA/U furin activity. 
   
   
       6 . The composition of  claim 1  comprising recombinant furin at an activity of at least about 100 U/μg and CHO host cell protein at a concentration less than about 11 μg protein/mL. 
   
   
       7 . The composition of  claim 1  comprising recombinant furin at an activity of at least about 100 U/μg and CHO host cell protein at a concentration less than about 1.0 ng protein/U furin activity. 
   
   
       8 . The composition of  claim 1  comprising recombinant furin at an activity of at least about 100 U/μg and CHO host cell DNA at a concentration less than about 14 ng DNA/mL. 
   
   
       9 . The composition of  claim 1  comprising recombinant furin at an activity of at least about 100 U/μg and CHO host cell DNA at a concentration less than about 0.5 pg DNA/U furin activity. 
   
   
       10 . A method of making substantially animal protein-free recombinant furin comprising the step of growing a host cell transformed or transfected with a polynucleotide encoding furin in serum-free medium under conditions that permit secretion of the furin into the medium. 
   
   
       11 . The method of  claim 10  comprising the step of adapting the host cell to grow in medium with increasingly lower concentrations of serum until all serum is removed from the medium. 
   
   
       12 . The method of  claim 10  comprising transferring the host cell from growth in medium comprising serum to growth in serum-free medium. 
   
   
       13 . The method of  claim 11  or  12  wherein the host cell is a CHO cell. 
   
   
       14 . A method of using the composition of  claim 1  comprising the step of contacting a pro-protein with the composition under conditions to cleave a pro-peptide from the pro-protein to form a mature protein. 
   
   
       15 . The method of  claim 14  wherein the mature protein is von Willebrand Factor. 
   
   
       16 . The method of  claim 14  wherein the mature protein is Factor VIII.

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