US2009181453A1PendingUtilityA1

Mesoderm And Definitive Endoderm Cell Populations

Assignee: SINAI SCHOOL MEDICINEPriority: May 17, 2002Filed: Nov 17, 2008Published: Jul 16, 2009

Est. expiryMay 17, 2022(expired)· nominal 20-yr term from priority

A61P 7/00A61P 3/10A61P 9/00A61P 21/00A61P 19/00A61P 1/16C12N 2506/03C12N 5/0603C12N 2500/90C12N 2501/415C12N 5/0606C12N 5/067C12N 2501/115C12N 2501/16C12N 2501/385C12N 2506/02C12N 2501/155C12N 2501/148C12N 2501/40C12N 5/0607C12N 2501/12C12N 2501/11A01N 1/10C12N 5/0672A01K 67/00

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Claims

Abstract

The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Claims

exact text as granted — not AI-modified

1 . A developmentally normal embryonic stem cell in which a nucleic acid encoding a first selectable marker is operably linked to brachyury regulatory elements and wherein one brachyury allele is inactivated by said nucleic acid and said first selectable marker is expressed. 
     
     
         2 . The embryonic stem cell of  claim 1  in which a second nucleic acid encoding a selectable marker is present in the HNF3β locus. 
     
     
         3 . The stem cell of  claim 2  in which the second selectable marker is human CD4. 
     
     
         4 . The stem cell of  claim 1  in which the first selectable marker is green fluorescent protein. 
     
     
         5 . A developmentally normal embryonic stem cell in which a nucleic acid encoding green fluorescent protein is operably linked to brachyury regulatory elements, and wherein one brachyury allele is inactivated by said nucleic acid encoding green fluorescent protein.

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