Diagnostics and therapeutics for cardiovascular disorders

Abstract

The methods of the present invention relate to the diagnosis of cardiovascular disorders. In one aspect, the specification discloses methods for determining a subject's predisposition to increased risk for myocardial infarction.

1 . A method for determining a Caucasian subject's predisposition to increased risk for myocardial infarction, comprising the steps of:
 (a) providing a biological sample comprising genomic DNA from the subject;   (b) typing said DNA at IL-1B (+3877) loci and IL-1B (−511) loci; and   (c) determining whether said subject has a composite genotype comprising the allelic pattern of:
 (i) two copies of allele 1 of IL-1B (−511) and two copies of allele 2 of IL-1B (+3877); 
 (ii) one copy of allele 1 of IL-1B (−511), one copy of allele 2 of IL-1B (−511), and two copies of allele 1 of IL-1B (+3877); or 
 (iii) two copies of allele 2 of IL-1B (−511) and two copies of allele 1 of IL-1B (+3877); and 
   wherein the presence of any one of said allelic patterns indicates that said subject is predisposed to developing myocardial infarction.   
     
     
         2 . The method of  claim 1 , wherein the determining step comprises determining whether the subject has a composite genotype comprising the allelic pattern of two copies of allele 1 of IL-1B (−511) and two copies of allele 2 of IL-1B (+3877), wherein the presence of said allelic pattern indicates that said subject is predisposed to developing myocardial infarction. 
     
     
         3 . The method of  claim 1 , wherein the determining step comprises determining whether the subject has a composite genotype comprising the allelic pattern of one copy of allele 1 of IL-1B (−511), one copy of allele 2 of IL-1B (−511), and two copies of allele 1 of IL-1B (+3877), wherein the presence of said allelic pattern indicates that said subject is predisposed to developing myocardial infarction. 
     
     
         4 . The method of  claim 1 , wherein the determining step comprises determining whether the subject has a composite genotype comprising the allelic pattern of two copies of allele 2 of IL-1B (−511) and two copies of allele 1 of IL-1B (+3877), wherein the presence of said allelic pattern indicates that said subject is predisposed to developing myocardial infarction. 
     
     
         5 . The method of  claim 1 , wherein said subject has increased expression of IL-1β and C-reactive protein. 
     
     
         6 . The method of  claim 2 , wherein said subject has increased expression of IL-1β and C-reactive protein. 
     
     
         7 . The method of  claim 3 , wherein said subject has increased expression of IL-1β and C-reactive protein. 
     
     
         8 . The method of  claim 4 , wherein said subject has increased expression of IL-1β and C-reactive protein. 
     
     
         9 . A method for determining a Caucasian subject's predisposition to cardiovascular disease, comprising detecting an IL-1A (+4845) allele 2 and an IL-1B (+3954) allele 2, wherein the presence of said alleles indicates that said subject is predisposed to cardiovascular disease. 
     
     
         10 . The method of  claim 9 , further comprising identifying an IL-1B (+3877) allele 1 or an IL-1B (−511) allele 1. 
     
     
         11 . A method for determining an Asian subject's predisposition to cardiovascular disease, comprising detecting two copies of an IL-1B (+3877) allele 1, wherein the presence of said alleles indicates that said subject is predisposed to cardiovascular disease. 
     
     
         12 . The method of  claim 11 , wherein said subject is Korean, Chinese or Japanese. 
     
     
         13 . The method of  claim 11 , further comprising identifying an IL-1B (−511) allele 2. 
     
     
         14 . A method for determining an Asian male subject's predisposition to cardiovascular disease wherein said subject is 55 or more years of age, comprising detecting an IL-1B (+3877) allele 2 and two copies of IL-1B (−511) allele 1, wherein the presence of said alleles indicates that said subject is predisposed to cardiovascular disease. 
     
     
         15 . The method of  claim 14 , wherein said subject is Korean, Chinese or Japanese. 
     
     
         16 . A method for determining a Korean male subject's predisposition to cardiovascular disease, comprising detecting two copies of IL-1B (+3877) allele 1 and two copies of IL-1B (−511) allele 2, wherein the presence of said alleles indicates that said subject is predisposed to cardiovascular disease. 
     
     
         17 . The method of  claim 16 , wherein said subject has increased expression of C-reactive protein.