Nucleotide and amino acid sequences from Xenorhabdus bovienii strain Xs85831 and uses thereof

Abstract

The present invention relates to nucleic acid sequences from Xenorhabdus , and, in particular, to genomic DNA sequences, and to nucleotide sequences that encode insecticidal proteins from Xenorhabdus bovienii , strain Xs85831. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins, fragments of proteins, tRNA's, fragments of tRNA's, rRNA's, and fragments of rRNA's. In addition, proteins and fragments thereof and antibodies capable of binding specifically to the proteins are encompassed by the present invention. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, protein fragments, RNA's, and antibodies, for example, for gene identification and analysis, preparation of constructs, and protecting plants from insect infestations.

1 - 9 . (canceled) 
     
     
         10 . A substantially purified nucleic acid molecule comprising a nucleic acid sequence having at least 90% sequence identity with SEQ ID NO: 3015 or the complement thereof. 
     
     
         11 . The substantially purified nucleic acid molecule of  claim 10 , wherein said nucleic acid sequence has at least 95% sequence identity with SEQ ID NO: 3015 or the complement thereof. 
     
     
         12 . The substantially purified nucleic acid molecule of  claim 10 , wherein said nucleic acid sequence has at least 98% sequence identity with SEQ ID NO: 3015 or the complement thereof. 
     
     
         13 . The substantially purified nucleic acid molecule of  claim 10 , wherein said nucleic acid sequence is 100% identical to SEQ ID NO: 3015 or the complement thereof. 
     
     
         14 . A substantially purified polypeptide comprising an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 8328 or the complement thereof. 
     
     
         15 . The substantially purified polypeptide of  claim 14 , wherein said amino acid sequence has at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 8328 or the complement thereof. 
     
     
         16 . The substantially purified polypeptide of  claim 15 , wherein said amino acid sequence has at least 98% sequence identity with the amino acid sequence of SEQ ID NO: 8328 or the complement thereof. 
     
     
         17 . The substantially purified polypeptide of  claim 15 , wherein said amino acid sequence is 100% identical with the amino acid sequence of SEQ ID NO: 8328 or the complement thereof. 
     
     
         18 . A substantially purified nucleic acid molecule comprising a nucleic acid sequence having at least 90% sequence identity with SEQ ID NO: 3016 or the complement thereof. 
     
     
         19 . The substantially purified nucleic acid molecule of  claim 18 , wherein said nucleic acid sequence has at least 95% sequence identity with SEQ ID NO: 3016 or the complement thereof. 
     
     
         20 . The substantially purified nucleic acid molecule of  claim 18 , wherein said nucleic acid sequence has at least 98% sequence identity with SEQ ID NO: 3016 or the complement thereof. 
     
     
         21 . The substantially purified nucleic acid molecule of  claim 18 , wherein said nucleic acid sequence is 100% identical to SEQ ID NO: 3016 or the complement thereof. 
     
     
         22 . A substantially purified polypeptide comprising an amino acid sequence having at least 90% sequence identity with the sequence amino acid sequence of SEQ ID NO: 8329 or the complement thereof. 
     
     
         23 . The substantially purified polypeptide of  claim 22 , wherein said amino acid sequence has at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 8329 or the complement thereof. 
     
     
         24 . The substantially purified polypeptide of  claim 22 , wherein said amino acid sequence has at least 98% sequence identity with the amino acid sequence of SEQ ID NO: 8329 or the complement thereof. 
     
     
         25 . The substantially purified polypeptide of  claim 22 , wherein said amino acid sequence is 100% identical with the amino acid sequence of SEQ ID NO: 8329 or the complement thereof. 
     
     
         26 . A transgenic plant comprising in its genome a substantially purified nucleic acid molecule which comprises:
 (1) a 5′ non-coding sequence which functions in a plant to cause the production of a mRNA molecule; which is operably linked to   (2) a nucleotide sequence encoding a polypeptide having an amino acid sequence with at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 8328 or the complement thereof; which is linked to   (3) a 3′ non-translated sequence that functions in said plant to cause termination of transcription.   
     
     
         27 . A transgenic plant comprising in its genome substantially purified nucleic acid molecule which comprises:
 (1) a 5′ non-coding sequence which functions in a plant to cause the production of a mRNA molecule; which is operably linked to   (2) a nucleotide sequence encoding a polypeptide having an amino acid sequence with at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 8329 or the complement thereof; which is linked to   (3) a 3′ non-translated sequence that functions in said plant to cause termination of transcription.   
     
     
         28 . A transformed seed comprising a nucleotide sequence encoding a polypeptide having an amino acid sequence with at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 8328 or the complement thereof. 
     
     
         29 . A transformed seed comprising a nucleotide sequence encoding a polypeptide having an amino acid sequence with at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 8329 or the complement thereof.