Cytotoxic protein and utlization thereof

Abstract

This invention relates to a new cytotoxic protein (M toxin, mucous layer devastating toxin) produced by Helicobacter pylori and the use. This invention provides a cytotoxic protein (M toxin) produced by Helicobacter pylori , a partial peptide, and an antitumor agent containing the cytotoxic protein. The protein is obtained by culturing a transformant which is transformed with a recombination vector containing DNA coding the cytotoxin protein. This invention provides further the use of the protein.

1 . A monoclonal antibody specific against a cytotoxic protein, which monoclonal antibody is obtained by immunizing a mammal with the cytotoxic protein of SEQ ID NO:1. 
     
     
         2 . The monoclonal antibody of  claim 1  which is produced by a hybridoma clone under the deposit accession no. FERM BP-8222. 
     
     
         3 . The monoclonal antibody of  claim 1  which is produced by a hybridoma clone under the deposit accession no. FERM BP-8223. 
     
     
         4 . The monoclonal antibody of  claim 1  which is by a hybridoma clone under the deposit accession no. FERM BP-8224. 
     
     
         5 . A polyclonal antibody specific against a cytotoxic protein, which polyclonal antibody is obtained by immunizing a mammal with the cytotoxic protein of SEQ ID NO:1. 
     
     
         6 . A method for detecting the cytotoxic protein of SEQ ID NO:1 in a sample, comprising:
 reacting the monoclonal antibody of  claim 1  with the sample to bind any cytotoxic protein of SEQ ID NO:1 present in the sample; and   detecting the binding of the monoclonal antibody to protein in the sample to detect the presence of the cytotoxic protein of SEQ ID NO:1.