Avian transgenesis using an ovalbumin nucleotide sequence

Abstract

The invention includes delivering to an avian a nucleic acid molecule which includes nucleotide sequence of an ovalbumin gene expression controlling region and a heterologous coding sequence operably linked to the gene expression controlling region wherein the heterologous coding sequence is expressed in a cell of the avian.

1 . A method comprising:
 delivering to an avian cell a nucleic acid molecule comprising a nucleotide sequence about 95% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1 operably linked to a heterologous coding sequence.   
     
     
         2 . The method of  claim 1  wherein the cell is an oviduct cell. 
     
     
         3 . The method of  claim 1  wherein the cell is a tubular gland cell. 
     
     
         4 . The method of  claim 1  wherein the nucleic acid molecule comprises a vector. 
     
     
         5 . The method of  claim 1  wherein the cell is a cell in culture. 
     
     
         6 . The method of  claim 1  wherein the cell is an in vivo cell of an avian. 
     
     
         7 . The method of  claim 1  wherein the nucleic acid molecule comprises an internal ribosome entry site (IRES). 
     
     
         8 . The method of  claim 1  wherein the nucleic acid integrates into the genome of the avian cell. 
     
     
         9 . The method of  claim 1  wherein the avian cell is selected from the group consisting of a chicken cell and a quail cell. 
     
     
         10 . The method of  claim 1  wherein the avian cell secretes a protein encoded by the heterologous coding sequence. 
     
     
         11 . The method of  claim 10  wherein the protein is isolated. 
     
     
         12 . The method of  claim 1  wherein the nucleotide sequence is about 99% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1. 
     
     
         13 . The method of  claim 1  wherein the nucleotide sequence is identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1. 
     
     
         14 . A method comprising delivering to an avian a nucleic acid molecule comprising a nucleotide sequence 95% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1 wherein the nucleic acid comprises a heterologous coding sequence operably linked to the nucleotide sequence and the heterologous coding sequence is expressed in a cell of the avian. 
     
     
         15 . The method of  claim 14  wherein the cell secretes a protein encoded by the heterologous coding sequence which is expressed in the cell. 
     
     
         16 . The method of  claim 15  wherein the protein is isolated. 
     
     
         17 . The method of  claim 14  wherein the cell is the cell of an avian selected from the group consisting of a chicken and a quail. 
     
     
         18 . The method of  claim 14  wherein the cell is an oviduct cell. 
     
     
         19 . The method of  claim 14  wherein the cell is a tubular gland cell. 
     
     
         20 . The method of  claim 19  wherein the tubular cell secretes a protein encoded by the heterologous coding sequence. 
     
     
         21 . The method of  claim 20  wherein the protein is secreted in egg white. 
     
     
         22 . The method of  claim 21  wherein the protein is isolated. 
     
     
         23 . The method of  claim 22  wherein the nucleotide sequence is about 99% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1. 
     
     
         24 . The method of  claim 22  wherein the nucleotide sequence is identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1. 
     
     
         25 . A method comprising introducing into an avian embryo cell a nucleic acid molecule comprising a nucleotide sequence 95% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1. 
     
     
         26 . The method of  claim 25  wherein the nucleotide sequence is about 99% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.