Fusion protein microarrays and methods of use

Abstract

The present invention provides a microarray having one or more fusion proteins non-covalently attached to a solid support. Non-covalent attachment is achieved by designing a fusion protein having a polyanionic domain attached to a subject protein, and attaching the fusion protein to a solid support having a polycationic coating.

1 .- 38 . (canceled) 
     
     
         39 . An array of subject proteins produced by a method comprising:
 (a) fusing to the termini of each subject protein an artificial polyanionic protein thereby forming fused proteins;   (b) applying a polycationic coating to the solid support; and   (c) dispensing each fused protein in solution to the solid support such that each fused protein solution is located in a discrete identifiable droplet on the solid support;   thereby producing an array of the subject proteins.   
     
     
         40 . A microarray comprising:
 (a) a solid support having a polycationic coating; and   (b) one or more fusion proteins non-covalently attached to the solid support in orderly discrete spots.   
     
     
         41 . The microarray of  claim 40 , wherein the solid support is a glass slide. 
     
     
         42 . The microarray of  claim 40 , wherein the polycationic coating is a polyamino acid. 
     
     
         43 . The microarray of  claim 42 , wherein the polyamino acid is poly-L-lysine. 
     
     
         44 . The microarray of  claim 40 , wherein the fusion protein comprises a subject protein and a polyanionic domain. 
     
     
         45 . The microarray of  claim 44 , wherein the polyanionic domain has the formula -[-(Ala-Gly) x -Pro-Glu-Gly-]- n . 
     
     
         46 . The microarray of  claim 45 , wherein x is 0, 1, 2, 3, 4, 5, 6, 7 or 8 and n is an integer between 1 and 40. 
     
     
         47 . The microarray of  claim 46 , wherein x is 3 and n is 16. 
     
     
         48 . The microarray of  claim 46 , wherein x is 3 and n is 36. 
     
     
         49 . The microarray of  claim 44 , wherein the polyanionic protein has the formula -[-(Ala-Gly) x -Glu-Gly-]- n . 
     
     
         50 . The microarray of  claim 49 , wherein x is 0, 1, 2, 3, 4, 5, 6, 7 or 8, and n is an integer between 1 and 40. 
     
     
         51 . The microarray of  claim 49 , wherein x is 4 and n is 16. 
     
     
         52 . The microarray of  claim 49 , wherein x is 4 and n is 18. 
     
     
         53 . The microarray of  claim 49 , wherein x is 4 and n is 28. 
     
     
         54 . The microarray of  claim 49 , wherein x is 5 and n is 14. 
     
     
         55 . The microarray of  claim 49 , wherein x is 6 and n is 14. 
     
     
         56 . (canceled) 
     
     
         57 . The array of  claim 39 , wherein the artificial polyanionic protein has the formula -[-(Ala-Gly) x -Pro-Glu-Gly-]- n . 
     
     
         58 . The array of  claim 39 , wherein the artificial polyanionic protein has the formula -[-(Ala-Gly) x -Glu-Gly-]- n .