Compositions and methods for controlling insects

Abstract

The present invention comprises compositions, methods and cell lines related to controlling insects. An embodiment of a composition comprises a plant essential oil and targets at least one receptor of insects chosen from tyramine receptor, Or83b olfactory receptor, and Or43a olfactory receptor, resulting in a change in the intracellular levels of cAMP, Ca2+, or both in the insects.

1 . A method of pest control, comprising:
 providing a composition comprising at least two active ingredients; and   contacting a pest with the composition, wherein the contacting results in a change in intracellular levels of cAMP, Ca 2+ , or both in said pest, and said change results in synergistic pest control.   
     
     
         2 . A method of pest control, comprising:
 providing a composition comprising at least two active ingredients, wherein at least one active ingredient is a ligand of a G-protein-coupled receptor in a target pest; and   contacting the pest with the composition, wherein the contacting results in synergistic pest control.   
     
     
         3 . The method of  claim 2 , wherein only one active ingredient is a ligand of the G-protein-coupled receptor. 
     
     
         4 . The method of  claim 2 , wherein the at least one active ingredient is a ligand of a G-protein-coupled receptor selected from the group consisting of a pest tyramine receptor, and pest Or43a receptor, and a pest Or83b receptor. 
     
     
         5 . The method of  claim 4 , wherein only one active ingredient is a ligand of the G-protein-coupled receptor. 
     
     
         6 . The method of  claim 2 , wherein the at least one active ingredient is a ligand of at least two G-protein-coupled receptors selected from the group consisting of a pest tyramine receptor, and pest Or43a receptor, and a pest Or83b receptor. 
     
     
         7 . The method of  claim 6 , wherein only one active ingredient is a ligand of the at least two G-protein-coupled receptors. 
     
     
         8 . The method of  claim 2 , wherein the at least two active ingredients are selected from the group consisting of: black seed oil, camphene, carvacrol, d-carvone, l-carvone, 1,8-cineole, p-cymene, diethyl phthalate, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil, lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, quinone, sabinene, α-terpinene, terpinene 900, gamma-terpineol, 2-tert-butyl-p-quinone, α-thujone, allyl sulfide, allyl trisulfide, allyl-disulfide, anethole,  artemisia  alcohol acetate, benzyl acetate, bergamotene, β-bisabolene, bisabolene oxide, α-bisabolol, bisabolol oxide, bisobolol oxide β, bornyl acetate, β-bourbonene, α-cadinol, α-campholene, α-campholene aldehyde, camphor, caryophyllene oxide, chamazulene, cinnamaldehyde, cis-verbenol, citral A, citral B, citronellol, citronellyl acetate, citronellyl formate, α-copaene, commint oil, β-costol, cryptone, curzerenone, davanone, diallyl tetrasulfide, dihydropyrocurzerenone, β-elemene, gamma-elemene, elmol, estragole, 2-ethyl-2-hexen-1-ol, eugenol acetate, α-farnesene, (Z,E)-α-farnesene, E-β-farnesene, fenchone, furanodiene, furanoeudesma-1,3-diene, furanoeudesma-1,4-diene, furano germacra 1,10(15)-diene-6-one, furanosesquiterpene, geraniol acetate, germacrene D, germacrene B, α-gurjunene, α-humulene, α-ionone, β-ionone, isoborneol, isofuranogermacrene, iso-menthone, iso-pulegone, jasmone, limonene, linalyl acetate, lindestrene, methyl-allyl-trisulfide, menthol, 2-methoxy furanodiene, menthone, menthyl acetate, methyl cinnamate, myrtenal neraldimethyl acetate, nerolidol, nonanone, 1-octanol, E ocimenone, Z ocimenone, 3-octanone, ocimene, octyl acetate, peppermint oil, α-phellandrene, β-phellandrene, prenal, pulegone, sabinyl acetate, α-santalene, santalol, sativen, δ-selinene, β-sesquphelandrene, spathulenol, tagetone, 4-terpineol, α-terpinolene, α-terpinyl acetate, α-thujene, thymyl methyl ether, trans-caryophyllene, trans-pinocarveol, trans-verbenol, verbenone, yomogi alcohol, zingiberene, and dihydrotagentone. 
     
     
         9 . The method of  claim 1 , wherein said synergistic pest control comprises a repellent effect. 
     
     
         10 . The method of  claim 1 , wherein said synergistic pest control comprises a pesticidal effect. 
     
     
         11 . The method of  claim 1 , wherein said composition further comprises at least one fixed oil. 
     
     
         12 . The method of  claim 11 , wherein the at least one fixed oil selected from the group consisting of castor oil, corn oil, cumin oil, mineral oil, olive oil, peanut oil, safflower oil, sesame oil, and soy bean oil. 
     
     
         13 . The method of  claim 1 , wherein the at least two active ingredients comprise geraniol and thyme oil. 
     
     
         14 . The method of  claim 13 , wherein the at least two active ingredients additionally comprise d-limonene. 
     
     
         15 . The method of  claim 14 , wherein the at least two active ingredients additionally comprise piperonal. 
     
     
         16 . The method of  claim 15 , wherein the at least two active ingredients additionally comprise linalool. 
     
     
         17 . The method of  claim 15 , wherein the at least two active ingredients additionally comprise lime oil. 
     
     
         18 . The method of  claim 1 , wherein the at least two active ingredients comprise lilac flower oil and d-limonene. 
     
     
         19 . The method of  claim 18 , wherein the at least two active ingredients further comprise thyme oil. 
     
     
         20 . The method of  claim 18 , wherein the at least two active ingredients further comprise lime oil. 
     
     
         21 . The method of  claim 1 , wherein the at least two active ingredients comprise d-limonene, linalool, piperonal, and geraniol. 
     
     
         22 . The method of  claim 1 , wherein the at least two active ingredients comprise linalool, thymol, α-pinene, and p-cymene. 
     
     
         23 . The method of  claim 23 , wherein the at least two active ingredients further comprise t-anethole.