Sequence analysis of the tetravalent rotavirus vaccine

Abstract

Isolated nucleic acid molecules comprising a gene segment from a rhesus rotavirus (RRV) or from one of three rhesus:human reassortant viruses are disclosed, including isolated nucleic acid molecules having a sequence selected from the group consisting of: SEQ ID NO: 1-14, inclusive, and isolated nucleic acid molecules encoding a protein having a sequence selected from the group consisting of SEQ ID NO: 15-28, inclusive, as well as variants of the isolated nucleic acid molecules.

1 . An isolated nucleic acid molecule having a sequence selected from the group consisting of: SEQ ID NO:1-14, inclusive. 
     
     
         2 . An isolated nucleic acid molecule encoding a polypeptide having a sequence selected from the group consisting of: SEQ ID NO: 15-28, inclusive. 
     
     
         3 . An isolated nucleic acid molecule having the sequence of SEQ ID NO: 1 and having a nucleotide change to a G at nucleotide 2120 
     
     
         4 . An isolated nucleic acid molecule having the sequence of SEQ ID NO: 2 and having one or more of the following nucleotide changes: a C at nucleotide 493, and a T at nucleotide 947. 
     
     
         5 . An isolated nucleic acid molecule having the sequence of SEQ ID NO:3 and having one or more of the following nucleotide changes: an A at nucleotide 169, a C at nucleotide 283, a C at nucleotide 448, a C at nucleotide 874, a C at nucleotide 1306, and a C at nucleotide 2388. 
     
     
         6 . An isolated nucleic acid molecule having the sequence of SEQ ID NO:4 and having one or more of the following nucleotide changes: a C at nucleotide 119, a G at nucleotide 417, a G at nucleotide 809, a C at nucleotide 977, an A at nucleotide 1463, a C at nucleotide 1481, a C at nucleotide 1608, a C at nucleotide 1755, and an A at nucleotide 1953. 
     
     
         7 . An isolated nucleic acid molecule having the sequence of SEQ ID NO:5 and having one or more of the following nucleotide changes: an A at nucleotide 75, a T at nucleotide 84, a C at nucleotide 347, a T at nucleotide 667, a C at nucleotide 1186, a G at nucleotide 1219, and an A at nucleotide 1204. 
     
     
         8 . An isolated nucleic acid molecule having the sequence of SEQ ID NO: 6 and having one or more of the following nucleotide changes: a C at nucleotide 376, an A at nucleotide 756, an A at nucleotide 1008, and a G at nucleotide 1041. 
     
     
         9 . An isolated nucleic acid molecule having the sequence of SEQ ID NO: 7 and having a nucleotide change to a G at nucleotide 387. 
     
     
         10 . An isolated nucleic acid molecule having the sequence of SEQ ID NO:10 and having one or more of the following nucleotide changes: an A at nucleotide 92, an A at nucleotide 174, and a G at nucleotide 218. 
     
     
         11 . An isolated nucleic acid molecule having the sequence of SEQ ID NO: 11 and having a nucleotide change to A at nucleotide 180. 
     
     
         12 . An isolated nucleic acid molecule having the sequence of SEQ ID NO: 12 and having a nucleotide change to an A at nucleotide 556. 
     
     
         13 . An isolated nucleic acid molecule having the sequence of SEQ ID NO: 14 and having a nucleotide change to a G at nucleotide 263. 
     
     
         14 . A vector comprising an isolated nucleic acid molecule of  claim 1 . 
     
     
         15 . A recombinant host cell comprising a vector of  claim 14 . 
     
     
         16 . A method for producing a polypeptide encoded by an isolated nucleic acid molecule, comprising culturing the recombinant host cell of  claim 15  under conditions suitable for expression of said nucleic acid molecule.