50 Human Secreted Proteins

Abstract

The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating diseases, disorders, and/or conditions related to these novel human secreted proteins.

1 . An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
 (a) a polynucleotide encoding amino acid residues 1 to 303 of SEQ ID NO:86;   (b) a polynucleotide encoding amino acid residues 2 to 303 of SEQ ID NO: 86;   (c) a polynucleotide encoding amino acid residues 28 to 303 of SEQ ID NO: 86;   (d) a polynucleotide encoding the amino acid sequence of the full-length polypeptide, which amino acid sequence is encoded by the cDNA clone contained in ATCC™ Deposit No. 209224, which was deposited on Aug. 28, 1997;   (e) a polynucleotide encoding the amino acid sequence of the full-length polypeptide, excluding the N-terminal methionine residue, which amino acid sequence is encoded by the cDNA clone contained in ATCC™ Deposit No. 209224, which was deposited on Aug. 28, 1997;   (f) a polynucleotide encoding the amino acid sequence of the mature polypeptide, which amino acid sequence is encoded by the cDNA clone contained in ATCC™ Deposit No. 209224, which was deposited on Aug. 28, 1997;   (g) a polynucleotide comprising a first polynucleotide 90% or more identical to a second polynucleotide sequence, wherein the second polynucleotide sequence is (a), (b), (c), (d), (e), or (f);   (h) polynucleotide encoding at least 30 contiguous amino acid residues of SEQ ID NO:86;   (i) a polynucleotide encoding at least 50 contiguous amino acid residues of SEQ ID NO: 86;   (j) a polynucleotide encoding at least 30 contiguous amino acid residues of the complete polypeptide encoded by the cDNA clone contained in ATCC™ Deposit No. 209224;   (k) a polynucleotide encoding at least 50 contiguous amino acid residues of the complete polypeptide encoded by the HKGAJ54 cDNA contained in ATCC™ Deposit No. 209224; and   (l) a polynucleotide comprising a polynucleotide which hybridizes to the complement of the polynucleotide set forth in SEQ ID NO: 31, wherein said hybridization occurs under conditions consisting essentially of hybridization in a buffer consisting of 50% formamide, 5×SSC, 50 mM sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA at 42° C. and wash in a solution consisting of 0.1×SSC at 65° C.   
     
     
         2 . The isolated nucleic acid molecule of  claim 1  wherein the polynucleotide further comprises a heterologous polynucleotide. 
     
     
         3 . The isolated nucleic acid molecule of  claim 2  wherein said heterologous polynucleotide encodes a heterologous polypeptide. 
     
     
         4 . The isolated nucleic acid molecule of  claim 3 , wherein said heterologous polypeptide is the Fc domain of immunoglobin. 
     
     
         5 . A recombinant vector, comprising the isolated nucleic acid molecule of  claim 1 . 
     
     
         6 . The recombinant vector of  claim 5 , wherein the nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression. 
     
     
         7 . A method of producing a recombinant vector, comprising inserting the isolated nucleic acid molecule of  claim 1  into a vector. 
     
     
         8 . A recombinant host cell, comprising the isolated nucleic acid molecule of  claim 1 . 
     
     
         9 . The recombinant host cell of  claim 8 , wherein the nucleic acid molecule is operably associated with a heterologous regulatory sequence that controls gene expression. 
     
     
         10 . A recombinant host cell, comprising the recombinant vector of  claim 5 . 
     
     
         11 . A method of producing a host cell, comprising transducing, transforming or transfecting a host cell with the vector of  claim 5 . 
     
     
         12 . A method for producing a protein, comprising:
 (a) culturing the host cell of  claim 8  under conditions suitable to produce a polypeptide encoded by the nucleic acid molecule; and   (b) recovering the protein from the cell culture.   
     
     
         13 . A composition, comprising the polynucleotide of  claim 1  and a pharmaceutically acceptable carrier.