Genes associated with mechanical stress, expression products therefrom, and uses thereof

Abstract

The disclosure relates to human and mechanical stress induced genes, in particular gene 608, and functional equivalents, probes therefor, tests to identify such genes, polypeptide expression products of such genes, antibodies to the polypeptides, uses for such genes, expression products and antibodies, e.g., in diagnosis (for instance risk determination), treatment, prevention, or control, of osteoporosis or fractures; and to diagnostic, treatment, prevention, or control methods or processes, as well as compositions therefor and methods or processes for making and using such compositions, and receptors therefor and methods or processes for obtaining and using such receptors.

1 . An isolated polypeptide encoded by a nucleic acid molecule comprising consecutive nucleotides having a sequence set forth in SEQ ID NO:6, SEQ ID NO: 20, SEQ ID NO: 23, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29 or SEQ ID NO:31 or comprising nucleotides having a sequence incorporated in plasmids deposited under ATCC Accession Nos. PTA-3638, PTA-3876, PTA-3877, PTA-3878 complements thereof or a polynucleotide having a sequence that differs due to the degeneracy of the genetic code from SEQ ID NO:6, SEQ ID NO: 20, SEQ ID NO: 23, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:31 or a sequence incorporated in plasmids deposited under ATCC Accession Nos. PTA-3638, PTA-3876, PTA-3877 or PTA-3878, or a sequence which hybridizes under stringent conditions to SEQ ID NO:6, SEQ ID NO: 20, SEQ ID NO: 23, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29 or SEQ ID NO:31 or to a sequence incorporated in plasmids deposited under ATCC Accession Nos. PTA-3638, PTA-3876, PTA-3877 or PTA-3878 or a functional portion thereof or a polynucleotide which is at least substantially homologous thereto. 
     
     
         2 . The isolated polypeptide of  claim 1 , wherein the polypeptide is identified as human protein 608, or a functional portion of protein 608 or Adlican-2, or a polypeptide which is at least substantially homologous thereto. 
     
     
         3 . The isolated polypeptide of  claim 2 , wherein the polypeptide comprises about 663 to about 1634 amino acids. 
     
     
         4 . An antibody which specifically binds to a polypeptide of  claim 2  or a functional portion thereof. 
     
     
         5 . An isolated polypeptide wherein the functional portion comprises consecutive amino acids having a sequence set forth in SEQ ID NO:16, SEQ ID NO: 24, SEQ ID NO: 30 or SEQ ID NO: 32. 
     
     
         6 . The isolated polypeptide of  claim 5  wherein the sequence comprises about the first 663 amino acids of the sequence set forth in SEQ ID NO:16, SEQ ID NO: 24, SEQ ID NO: 30 or SEQ ID NO: 32. 
     
     
         7 . The isolated polypeptide of  claim 6  wherein the sequence comprises about the first 741 amino acids of the sequence set forth in SEQ ID NO:16, SEQ ID NO: 24, SEQ ID NO: 30 or SEQ ID NO: 32. 
     
     
         8 . The isolated polypeptide of  claim 5 , wherein the polypeptide is identified as human 608 protein or human Adlican-2 protein or a functional portion thereof or a polypeptide which is at least substantially homologous thereto. 
     
     
         9 . An isolated polypeptide of  claim 5  comprising consecutive amino acids having a sequence set forth in SEQ ID NO:32, designated human 608 protein. 
     
     
         10 . An isolated polypeptide of  claim 5  comprising consecutive amino acids having a sequence set forth in SEQ ID NO: 30 designated Adlican-2 polypeptide. 
     
     
         11 . An isolated polypeptide of  claim 10  comprising consecutive amino acids having a sequence set forth in SEQ ID NO: 30 deleted of amino acids 6-215. 
     
     
         12 . An antibody which binds specifically to a polypeptide of  claim 9 . 
     
     
         13 . An antibody which binds specifically to a polypeptide of  claim 10 . 
     
     
         14 . An antibody of  claim 12 , which does not bind specifically to a rat 608 polypeptide. 
     
     
         15 . An antibody of  claim 14 , wherein the rat polypeptide has a sequence set forth in SEQ ID NO: 34. 
     
     
         16 . A composition comprising the antibody of  claim 14