Sequences characteristic of hypoxia-regulated gene transcription

Abstract

There are provided polynucleotides that are modulated by hypoxic conditions. The disclosure includes such genes and proteins as well as analogs, salts and functional derivatives of such proteins, and DNA encoding such analogs, and methods of use. Methods for treating the effects of stroke, hypoxia and/or ischemia by regulating such genes or proteins are also disclosed. The presence of hypoxia or a hypoxia-associated pathology may be diagnosed by screening for the presence of at least one polynucleotide having the nucleic acid sequence according to the present invention. Methods of regulating hypoxia associated pathologies are also provided.

1 . A polynucleotide comprising the sequences of any one of SEQ ID NOs:1, 3, 5, 7, 9, 10, 12, 14, 16, 18, and 19. 
     
     
         2 . An isolated polynucleotide having the sequence of
 (a) any one of SEQ ID NOs: 1, 3, 5, 7, 9, 10, 12, 14, 16, 18, and 19;   (b) a naturally-occurring polynucleotide comprising a sequence of (a); or   (c) a naturally-occurring polynucleotide having at least 70% identity with a naturally-occurring polynucleotide of (b) and, in naturally-occurring neural cells, has its expression modulated when the cells are subjected to neurotoxic stress;   (d) a naturally-occurring polynucleotide capable of hybridizing under moderately stringent conditions to a naturally-occurring polynucleotide of (b) and, in naturally-occurring neural cells, has its expression modulated when the cells are subjected to neurotoxic stress;   (e) a fragment of a polynucleotide of (a), (b), (c) or (d) having at least 20 nucleotides; or   (f) a polynucleotide sequence complementary to a polynucleotide of (a), (b), (c), (d) or (e).   
     
     
         3 . An isolated polynucleotide in accordance with  claim 2 , wherein said sequence of (a) is SEQ ID NO:3. 
     
     
         4 . An isolated polynucleotide in accordance with  claim 2  comprising a strand of a full-length cDNA. 
     
     
         5 . An isolated polynucleotide in accordance with  claim 3  comprising a strand of a full-length cDNA. 
     
     
         6 . An isolated polynucleotide encoding the polypeptide of SEQ ID NO:4.