Correction of Oxygen Effect in Test Sensor Using Reagents

Abstract

An electrochemical test sensor is adapted to measure glucose and correct for the oxygen effect in a fluid sample. The test sensor comprises a base, first and second working electrodes, and a counter electrode. The first working electrode includes glucose oxidase, a mediator and peroxidase. The second working electrode includes glucose oxidase and the mediator. The first working electrode, the second working electrode and the counter electrode are located on the base. In other embodiments, an electrochemical test sensor is adapted to measure cholesterol, lactate, pyruvate or xanthine and correct for the oxygen effect in a fluid sample.

1 . An electrochemical test sensor adapted to measure glucose and correct for the oxygen effect in a fluid sample, the test sensor comprising:
 a base;   a first working electrode including glucose oxidase, a mediator and peroxidase;   a second working electrode including glucose oxidase and the mediator; and a   a counter electrode,   wherein the first working electrode, the second working electrode and the counter electrode are located on the base.   
   
   
       2 . The test sensor of  claim 1 , wherein the mediator is a ferricyanide derivative. 
   
   
       3 . (canceled) 
   
   
       4 . The test sensor of  claim 1 , wherein the mediator is a ruthenium (III) derivative or a ferricium derivative. 
   
   
       5 . The test sensor of  claim 1 , wherein the mediator is an inorganic mediator. 
   
   
       6 . An electrochemical test sensor adapted to measure glucose and correct for the oxygen effect in a fluid sample, the test sensor comprising:
 a base;   a first working electrode including glucose oxidase, a mediator and peroxidase;   a second working electrode including glucose oxidase and the mediator;   a counter electrode; and a   a lid being attached to the base,   wherein the first working electrode, the second working electrode and the counter electrode are located on either the base or the lid.   
   
   
       7 . The test sensor of  claim 6 , wherein the mediator is a ferricyanide derivative. 
   
   
       8 . (canceled) 
   
   
       9 . The test sensor of  claim 6 , wherein the mediator is a ruthenium (III) derivative or a ferricium derivative. 
   
   
       10 . The test sensor of  claim 6 , wherein the mediator is an inorganic mediator. 
   
   
       11 . The test sensor of  claim 6 , wherein the lid and the base are attached via an adhesive. 
   
   
       12 . An electrochemical test sensor adapted to measure glucose and correct for the oxygen effect in a fluid sample, the test sensor comprising:
 a base;   a first working electrode including glucose oxidase, a mediator and peroxidase;   a second working electrode including glucose oxidase and the mediator;   a counter electrode;   a lid; and   a spacer being located between the lid and the base,   wherein the first working electrode, the second working electrode and the counter electrode are located adjacent to the base.   
   
   
       13 . The test sensor of  claim 12 , wherein the mediator is a ferricyanide derivative. 
   
   
       14 . The test sensor of  claim 12 , wherein the mediator is a ruthenium (III) derivative or a ferricium derivative. 
   
   
       15 . The test sensor of  claim 12 , wherein the mediator is an inorganic mediator. 
   
   
       16 . A method for correcting the oxygen effect in determining the concentration of glucose using an electrochemical test sensor, the method comprising the acts of:
 providing a test sensor, the test sensor comprising a base, first and second working electrodes, and a counter electrode, the first working electrode including glucose oxidase, a mediator and peroxidase, the second working electrode including glucose oxidase and the mediator, the first working electrode, the second working electrode and the counter electrode being adjacent to the base;   contacting the test sensor to a meter to form an electrical connection;   placing a fluid on the test sensor;   measuring a first current from the first working electrode;   measuring a second current from the second working electrode; and   determining the concentration of glucose using the first current measurement and the second current measurement.   
   
   
       17 . The method of  claim 16 , wherein the fluid is blood. 
   
   
       18 . The method of  claim 16 , wherein the mediator is a ferricyanide derivative. 
   
   
       19 . (canceled) 
   
   
       20 . The method of  claim 16 , wherein the mediator is a ruthenium (III) derivative or a ferricium derivative. 
   
   
       21 . The method of  claim 16 , wherein the mediator is an inorganic mediator. 
   
   
       22 . (canceled) 
   
   
       23 . (canceled) 
   
   
       24 . The method of  claim 16 , wherein the test sensor further includes a lid and a spacer, the spacer being located between the base and the lid. 
   
   
       25 . An electrochemical test sensor adapted to measure cholesterol and correct for the oxygen effect in a fluid sample, the test sensor comprising:
 a base;   a first working electrode including cholesterol oxidase, a mediator and peroxidase;   a second working electrode including cholesterol oxidase and the mediator; and a   a counter electrode,   wherein the first working electrode, the second working electrode and the counter electrode are located on the base.   
   
   
       26 .- 48 . (canceled) 
   
   
       49 . An electrochemical test sensor adapted to measure at least one of lactate, pyruvate or xanthine and correct for the oxygen effect in a fluid sample, the test sensor comprising:
 a base;   a first working electrode including lactate, pyruvate or xanthine oxidase, a mediator and peroxidase;   a second working electrode including lactate, pyruvate or xanthine oxidase and the mediator; and a   a counter electrode,   wherein the first working electrode, the second working electrode and the counter electrode are located on the base.   
   
   
       50 .- 72 . (canceled)