Method for treating or preventing conditions by elevation of the adenosine level in an individual

Abstract

This invention concerns a method of inducing an elevated level of adenosine in an individual by administering to the individual an effective amount of either i) recombinant protein CD73 or ii) a cytokine or another factor being capable of inducing endothelial CD73 expression, or a combination thereof. In addition, the invention concerns a method for prevention or treatment of a disease or disorder requiring or benefiting from the elevation of the adenosine level in an individual. Furthermore, the invention concerns a method of up-regulating endothelial CD73 expression in an individual by administering to the individual an effective amount of a cytokine or another factor being capable of inducing endothelial CD73 expression, and to a method for prevention or treatment of a disease or disorder requiring or benefiting from up-regulating endothelial CD73 expression.

1 . A method of inducing an elevated level of adenosine in an individual by administering to said individual an effective amount of either i) recombinant protein CD73 or ii) a cytokine or another factor being capable of inducing endothelial CD73 expression, or a combination thereof. 
   
   
       2 . The method according to  claim 1  wherein the elevated level of adenosine is achieved by up-regulating endothelial CD73 expression in the individual by administering to the individual an effective amount of a cytokine. 
   
   
       3 . The method according to  claim 1  wherein the cytokine is an interferon or an interleukin. 
   
   
       4 . The method according to  claim 3  wherein the interferon is interferon alpha, beta, gamma, omega, or any subtype thereof. 
   
   
       5 . A method for prevention or treatment of a disease or disorder requiring or benefiting from the elevation of the adenosine level in an individual, by administering to said individual an effective amount of amount of either i) recombinant protein CD73 or ii) a cytokine or another factor being capable of inducing endothelial CD73 expression, or a combination thereof. 
   
   
       6 . The method according to  claim 5  wherein the elevated level of adenosine is achieved by up-regulating endothelial CD73 expression in the individual by administering to the individual an effective amount of a cytokine. 
   
   
       7 . The method according to  claim 5  wherein the cytokine is an interferon or an interleukin. 
   
   
       8 . The method according to  claim 7  wherein the interferon is interferon alpha, beta, gamma, omega, or any subtype thereof. 
   
   
       9 . The method according to  claim 5 , wherein the disease or disorder is
 a) tissue trauma,   b) a reperfusion injury resulting from myocardial infarction or stroke, organ transplantations or an other surgical operation,   c) cancer or cancer metastasis, or   d) an inflammatory condition.   
   
   
       10 . The method according to  claim 5  wherein an effective amount of adenosine monophosphate is administered to the individual. 
   
   
       11 . The method according to  claim 5  wherein an adenylate kinase inhibitor, which prevents AMP from conversion into adenosine diphosphate (ADP) or adenosine triphosphate (ATP) is administered. 
   
   
       12 . The method according to  claim 5  wherein an adenosine deaminase inhibitor which prevents the decomposition of adenosine, is administered. 
   
   
       13 . The method according to  claim 9 , wherein the disease or disorder is tissue trauma, a reperfusion injury resulting from myocardial infarction or stroke, cancer or cancer metastasis or an inflammatory disease and the administering of the recombinant protein CD73 or cytokine or another factor or a combination thereof is started immediately the patient is brought to care. 
   
   
       14 . The method according to  claim 9 , wherein the disease or disorder is a reperfusion injury resulting from organ transplantations or an other surgical operation and the administering of the recombinant protein CD73 or cytokine or another factor or a combination thereof is started before the surgical operation. 
   
   
       15 . A method of up-regulating endothelial CD73 expression in an individual by administering to said individual an effective amount of a cytokine or another factor being capable of inducing endothelial CD73 expression. 
   
   
       16 . The method according to  claim 15  wherein the cytokine is an interferon or an interleukin. 
   
   
       17 . The method according to  claim 16  wherein the interferon is interferon alpha, beta, gamma, omega, or any subtype thereof. 
   
   
       18 . A method for prevention or treatment of a disease or disorder requiring or benefiting from up-regulating endothelial CD73 expression in an individual, by administering to said individual an effective amount of a cytokine or another factor being capable of inducing endothelial CD73 expression. 
   
   
       19 . The method according to  claim 18  wherein the cytokine is an interferon or an interleukin. 
   
   
       20 . The method according to  claim 19  wherein the interferon is interferon alpha, beta, gamma, omega, or any subtype thereof.