Marker and method for cancer diagnosis

Abstract

The present invention relates to a diagnostic cancer marker using variation of a granulocyte colony stimulating factor (G-CSF) gene and a method for preparing the same, and more specifically, relates to a method for diagnosing cancer and/or assessing the state of cancer progression using an oligonucleotide having the 3′-terminal end of exon 2 region linked to the 5′-terminal end of exon 4 region of a G-CSF gene as a diagnostic cancer marker. According to the present invention, cancer can be quickly and exactly diagnosed using variation in a G-CSF gene expression.

1 . An oligonucleotide for diagnosing cancer, essentially containing a nucleic acid sequence of a splice junction site having the 3′-terminal end of exon 2 region linked to the 5′-terminal end of exon 4 region of a granulocyte colony stimulating factor gene. 
     
     
         2 . The oligonucleotide for diagnosing cancer according to  claim 1 , which essentially contains nucleic acid sequences of SEQ ID NOs: 1 or 2. 
     
     
         3 . A diagnostic kit for cancer diagnosis, containing the oligonucleotide of  claim 1 . 
     
     
         4 . The diagnostic kit for cancer diagnosis according to  claim 4 , wherein said diagnostic kit assesses the state of cancer progression and additionally contains an oligonucleotide essentially containing sequences of a part or the entire region of the exon 3 region of G-CSF gene. 
     
     
         5 . The diagnostic kit for cancer diagnosis according to  claim 4 , wherein said oligonucleotide essentially contains nucleic acid sequences of SEQ ID NOs: 1 or 2. 
     
     
         6 . The diagnostic kit for cancer diagnosis according to  claim 3 , wherein said kit is microarray. 
     
     
         7 . A method for diagnosing cancer, the method comprising the steps of:
 (a) obtaining a G-CSF nucleic acid sample from a mammal biological sample;   (b) amplifying the obtained G-CSF nucleic acid sample; and   (c) detecting oligonucleotide containing a nucleic acid sequence of a splice junction site having the 3′-terminal end of exon 2 region linked to the 5′-terminal end of exon 4 region of a G-CSF gene, in the amplifying sample.   
     
     
         8 . The method for diagnosing cancer according to  claim 7 , wherein the step (c) simultaneously detects an oligonucleotide containing sequences of a part or the entire region of the exon 3 region together with an oligonucleotide containing a nucleic acid sequence of splice junction site having the 3′-terminal end of exon 2 region linked to the 5′-terminal end of exon 4 region of a G-CSF gene, in the amplifying sample. 
     
     
         9 . The method for diagnosing cancer according to  claim 7 , wherein said oligonucleotide essentially contains nucleic acid sequences of SEQ ID NOs: 1 or 2.