Purified TNFR preparations

Abstract

Purified protein preparations comprising tumor necrosis factor are disclosed.

1 . A purified preparation of a protein,
 wherein the protein comprises amino acids 1-163 of SEQ ID NO:2,   wherein the protein has been purified by a method comprising the following steps:   (a) concentrating a supernatant from a mammalian cell culture which contains the protein;   (b) purifying the protein in the concentrated supernatant by subjecting it to affinity chromatography and/or ion exchange chromatography.   
     
     
         2 . The purified preparation of  claim 1 , wherein the protein comprises amino acids 1-185 of SEQ ID NO:2. 
     
     
         3 . The purified preparation of  claim 2 , wherein the protein comprises amino acids 1-235 of SEQ ID NO:2. 
     
     
         4 . The purified preparation of  claim 1 , wherein the mammalian cell culture is a CHO cell culture. 
     
     
         5 . The purified preparation of  claim 1 , wherein the protein has been subjected to affinity chromatography and ion exchange chromatography. 
     
     
         6 . The purified preparation of  claim 5 , wherein ion exchange chromatography is done after affinity chromatography. 
     
     
         7 . The purified preparation of  claim 5 , wherein the ion exchange chromatography is anion exchange chromatography. 
     
     
         8 . The purified preparation of  claim 7 , wherein the anion exchange chromatography is performed on a matrix having pendant diethylaminoethyl (DEAE) groups. 
     
     
         9 . The purified preparation of  claim 1 , wherein the ion exchange chromatography is anion exchange chromatography. 
     
     
         10 . The purified preparation of  claim 9 , wherein the anion exchange chromatography is performed on a matrix having pendant diethylaminoethyl (DEAE) groups. 
     
     
         11 . The purified preparation of  claim 1 , wherein the ion exchange chromatography is cation exchange chromatography. 
     
     
         12 . The purified preparation of  claim 11 , wherein the cation exchange chromatography is performed on a matrix having pendant carboxymethyl or sulfopropyl groups. 
     
     
         13 . The purified preparation of  claim 1 , wherein the purified preparation comprises less than about one percent by weight of non-human cell components. 
     
     
         14 . A purified preparation of a protein comprising less than about one percent by weight of non-human cell components, wherein the protein comprises amino acids 1-163 of SEQ ID NO:2. 
     
     
         15 . The purified preparation of  claim 14 , wherein the protein has been purified from a supernatant of a mammalian cell culture. 
     
     
         16 . The purified preparation of  claim 15 , wherein the protein has been purified by a method comprising the following steps:
 (a) concentrating the supernatant;   (b) purifying the protein in the concentrated supernatant by subjecting it to affinity chromatography.   
     
     
         17 . The purified preparation of  claim 15 , wherein the protein has been purified by a method comprising the following steps:
 (a) concentrating the supernatant;   (b) purifying the protein in the concentrated supernatant by subjecting it to ion exchange chromatography.   
     
     
         18 . The purified preparation of  claim 17 , wherein the ion exchange chromatography is anion exchange chromatography. 
     
     
         19 . The purified preparation of  claim 17 , wherein the ion exchange chromatography is cation exchange chromatography. 
     
     
         20 . The purified preparation of  claim 15 , wherein the mammalian cell culture is a CHO cell culture.