Polynucleotide encoding A polypeptide having heparanase activity and expression of same in genetically modified cells

Abstract

A polynucleotide (hpa) encoding a polypeptide having heparanase activity, vectors including same, genetically modified cells expressing heparanase, a recombinant protein having heparanase activity and antisense oligonucleotides and constructs for modulating heparanase expression.

1 - 13 . (canceled) 
     
     
         14 . A compound comprising a polypeptide at least 80% homologous to SEQ ID NO: 10 and wherein said compound is pure enough to elicit anti-heparanase antibodies. 
     
     
         15 . The compound of  claim 14 , wherein said polypeptide is at least 85% homologous to SEQ ID NO: 10. 
     
     
         16 . The compound of  claim 14 , wherein said polypeptide is at least 90% homologous to SEQ ID NO: 10. 
     
     
         17 . The compound of  claim 14 , wherein said polypeptide is at least 95% homologous to SEQ ID NO: 10. 
     
     
         18 . The compound of  claim 14 , which has heparanase catalytic activity or is cleavable so as to acquire said heparanase catalytic activity. 
     
     
         19 . The compound of  claim 14 , wherein said amino acid sequence has a phenylalanine residue corresponding to position 246 of SEQ ID NO:10. 
     
     
         20 . The compound of  claim 19 , wherein said polypeptide is at least 85% homologous to SEQ ID NO: 10. 
     
     
         21 . The compound of  claim 19 , wherein said polypeptide is at least 90% homologous to SEQ ID NO: 10. 
     
     
         22 . The compound of  claim 19 , wherein said polypeptide is at least 95% homologous to SEQ ID NO: 10. 
     
     
         23 . The compound of  claim 19 , which has heparanase catalytic activity or is cleavable so as to acquire said heparanase catalytic activity.