Cellooligosaccharide-fermentative zymobacter transformed microorganisms

Abstract

This invention provides transformed microorganisms which can produce ethanol from cellooligosaccharide, by introducing β-glucosidase gene by recombinant DNA method, into microorganisms belonging to genus Zymobacter which cannot utilize cellooligosaccharide.

1 - 7 . (canceled) 
     
     
         8 . An immobilization carrier comprising a transformed cellooligosaccharide-fermentative microorganism attached thereto, wherein said microorganism belongs to genus  Zymobacter palmae  strain and is transformed with an exogenous β-glucosidase gene. 
     
     
         9 . A bioreactor equipped with the immobilization carrier of  claim 8 . 
     
     
         10 . A process for producing ethanol, which comprises continuously fermenting cellobiose-containing saccharification material using the immobilization carrier of  claim 8 . 
     
     
         11 . The immobilization carrier of  claim 8 , wherein said exogenous gene is a polynucleotide encoding β-glucosidase obtained from  Ruminococcus albus  using PCR primers of SEQ ID No. 1 and SEQ ID No. 2. 
     
     
         12 . The immobilization carrier of  claim 8 , wherein said  Zymobacter palmae  strain is transformed with a polynucleotide encoding β-glucosidase obtained from  Ruminococcus albus  using PCR primers of SEQ ID No. 1 and SEQ ID No. 2, and said transformed strain having been deposited at National Institute of Advanced Industrial Science and Technology, International Patent Organism Depositary under deposit accession number FERM BP-10047.