Plant myb transcription factor homologs

Abstract

This invention relates to an isolated nucleic acid fragment encoding a Myb-related transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the Myb-related transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the Myb-related transcription factor in a transformed host cell.

1 - 16 . (canceled) 
     
     
         17 . An isolated polynucleotide comprising:
 (a) a nucleotide sequence encoding a polypeptide having Myb-related transcription factor activity, wherein the polypeptide has an amino acid sequence of at least 90% sequence identity when compared to SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60 or 62, based on the Clustal V method of alignment with pairwise alignment default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, or   (b) a full-length complement of the nucleotide sequence of (a).   
     
     
         18 . The polynucleotide of  claim 17 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity when compared to SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60 or 62, based on the Clustal V method of alignment with the pairwise alignment default parameters. 
     
     
         19 . The polynucleotide of  claim 17 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60 or 62. 
     
     
         20 . The polynucleotide of  claim 17  wherein the nucleotide sequence comprises SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59 or 61. 
     
     
         21 . A recombinant DNA construct comprising the polynucleotide of  claim 17  operably linked to at least one regulatory sequence. 
     
     
         22 . A method for transforming a cell, comprising transforming a cell with the polynucleotide of  claim 17 . 
     
     
         23 . A cell comprising the recombinant DNA construct of  claim 21 . 
     
     
         24 . A method for producing a transgenic plant comprising transforming a plant cell with the polynucleotide of  claim 17  and regenerating a transgenic plant from the transformed plant cell. 
     
     
         25 . A plant comprising the recombinant DNA construct of  claim 21 . 
     
     
         26 . A seed comprising the recombinant DNA construct of  claim 21 . 
     
     
         27 . A method for isolating a polypeptide encoded by the recombinant DNA construct of  claim 21 , wherein the method comprises the following:
 (a) transforming a cell with the recombinant DNA construct of  claim 21 ;   (b) growing the transformed cell of step (a) under conditions suitable for expression of the recombinant DNA construct; and   (c) isolating the polypeptide from the transformed cell of step (b).   
     
     
         28 . An isolated polynucleotide comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises a fusion protein partner covalently linked to the isolated polypeptide of  claim 17 . 
     
     
         29 . The isolated polynucleotide of  claim 28 , wherein the fusion protein partner is at least one selected from the group consisting of glutathione S-transferase, thioredoxin, maltose binding protein, and a hexahistidine polypeptide. 
     
     
         30 . An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the DNA-binding domain of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60 or 62, wherein the polypeptide has Myb-related transcription factor activity. 
     
     
         31 . An isolated polypeptide comprising an amino acid sequence of at least 90% sequence identity when compared to SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60 or 62, based on the Clustal V method of alignment with pairwise alignment default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, wherein the polypeptide has Myb-related transcription factor activity. 
     
     
         32 . The isolated polypeptide of  claim 31 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity when compared to SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60 or 62, based on the Clustal V method of alignment with the pairwise alignment default parameters. 
     
     
         33 . The isolated polypeptide of  claim 31 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60 or 62. 
     
     
         34 . An isolated fusion protein comprising a fusion protein partner covalently linked to the isolated polypeptide of  claim 31 . 
     
     
         35 . The isolated fusion protein of  claim 34 , wherein the fusion protein partner is at least one selected from the group consisting of glutathione S-transferase, thioredoxin, maltose binding protein, and a hexahistidine polypeptide. 
     
     
         36 . An isolated polypeptide comprising the DNA-binding domain of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60 or 62, wherein the polypeptide has Myb-related transcription factor activity.