Novel Lipase Genes

Abstract

New lipase enzymes (both nucleic acids and polypeptides) are provided. Compositions which include these polypeptides, proteins, nucleic acids, recombinant cells, as well as methods involving the enzymes, antibodies to the enzymes, and methods of using the enzymes are also provided.

1 . An isolated or recombinant polypeptide having lipase activity and comprising an amino acid sequence with at least 97% identity to any one of SEQ ID NOs:75 to SEQ ID NO:108. 
     
     
         2 . The isolated or recombinant polypeptide of  claim 1  in which the polypeptide exhibits lipase activity with respect to tributyrin, neryl-butyrate, or geranyl-butyrate. 
     
     
         3 . The isolated or recombinant polypeptide of  claim 1  in which the polypeptide has lipase activity with respect to methyl esters. 
     
     
         4 . The isolated or recombinant polypeptide of  claim 1  in which the polypeptide has lipase activity with respect to pentadecanolide. 
     
     
         5 . The isolated or recombinant polypeptide of  claim 1  in which the polypeptide has lipase activity with respect to oxacyclotridecan. 
     
     
         6 . The isolated or recombinant polypeptide of  claim 1 , wherein the polypeptide comprises an amino acid sequence with one or more amino acid residues selected from the group consisting of: Lys at position 1; Thr at position 14; Ser at position 17; Arg at position 22; Glu at position 26; Pro at position 31; Gly at position 33; Glu at position 34; Pro at position 35; Pro or Thr at position 37; Ser or Lys at position 41; Gly at position 42; Arg or Glu at position 43; Ala at position 61; Tyr at position 75; Gly at position 96; Ser at position 97; Thr at position 104; Ser at position 107; Ala at position 125; Gly at position 129; Val at position 134; Cys at position 138; Lys at position 141; Lys at position 146; Thr at position 156; Met at position 160, Arg at position 166, or His at position 177, wherein the positions are equivalent amino acid positions with respect to SEQ ID NO: 75. 
     
     
         7 . The isolated or recombinant polypeptide of  claim 1  in which the polypeptide is enantioselective for geranyl butyrate over neryl butyrate. 
     
     
         8 . The isolated or recombinant polypeptide of  claim 7  in which the polypeptide comprises an amino acid sequence with one or more of the following amino acid residues: Arg at position 22, Gly at position 33, Ser or Lys at position 41, Arg at position 43, Ser at position 107, Lys at position 141, Lys at position 146, Met at position 160, and His at position 177, wherein the positions are equivalent amino acid positions with respect to SEQ ID NO: 75. 
     
     
         9 . The isolated or recombinant polypeptide of  claim 1  in which the lipase has enantioselectivity for neryl butyrate versus geranyl butyrate. 
     
     
         10 . The isolated or recombinant polypeptide of  claim 9  in which the polypeptide comprises an amino acid sequence with one or more of the following amino acid residues: Ser at position 17, Arg at position 22, Pro at position 31, Gly at position 33, Ser or Lys at position 41, Lys at position 141, Lys at position 146, Met at position 160, Arg at position 166, or His at position 177, wherein the positions are equivalent amino acid positions with respect to SEQ ID NO: 75. 
     
     
         11 . An isolated or recombinant polypeptide having lipase activity and comprising an amino acid sequence with at least 94% identity to the mature region of SEQ ID NO: 61, 64, 65, 66, 68, 70 or 72. 
     
     
         12 . An isolated or recombinant polypeptide having lipase activity and comprising an amino acid sequence with at least 85% amino acid sequence identity to the mature region of SEQ ID NO: 74. 
     
     
         13 . An isolated or recombinant polypeptide having lipase activity and comprising an amino acid sequence with at least 99% identity to SEQ ID NO:56. 
     
     
         14 . The polypeptide of  claim 1 ,  11 ,  12 , or  13  comprising a leader sequence. 
     
     
         15 . The polypeptide of  claim 1 ,  11 ,  12 , or  13  comprising a secretion or localization signal. 
     
     
         16 . An isolated or recombinant nucleic acid encoding a polypeptide of  claim 1 . 
     
     
         17 . A vector comprising the isolated or recombinant nucleic acid of  claim 16 . 
     
     
         18 . The vector of  claim 17 , wherein the vector is selected from the group consisting of a plasmid, a cosmid, a phage, a virus, and a fragment of a virus. 
     
     
         19 . The vector of  claim 17  comprising a control sequence that controls expression of the encoded polypeptide. 
     
     
         20 . A host cell comprising the vector of  claim 17 . 
     
     
         21 . The host cell of  claim 20  which is a prokaryotic cell. 
     
     
         22 . The host cell of  claim 20  which is an eukaryotic cell. 
     
     
         23 . A method of producing a polypeptide having lipase activity, comprising:
 culturing a cell of  claim 20  in under conditions suitable for expression of the polypeptide encoded by the nucleic acid.   
     
     
         24 . The method of  claim 23 , further comprising isolating the polypeptide from the cells. 
     
     
         25 . The method of  claim 23 , further comprising isolating or recovering the polypeptide from the nutrient medium.