Dat1

Abstract

A synthetic diaminoketothiazole, its process of preparation and its use as a microtubule inhibitor, a probe for tubulin-microtubule system and a cytotoxic agent. Diaminoketothiazole of the formula (I) wherein Ar is 4-OMe-C 6 H 5 , Ar′ is C 6 H 5 .

1 . A method of microtubule inhibition comprising:
 administering a diaminoketothiazole (DAT1) having a formula:   
       
         
           
           
               
               
           
         
       
       wherein Ar is 4-OMe-C 6 H 5  and Ar′ is C 6 H 5 ; and wherein the DAT1 compound is a cytotoxic agent. 
     
     
         2 . The method of microtubule inhibition of  claim 1 , wherein the DAT1 distorts a microtubule in a cancer cell and inhibits in vitro assembly of a microtubular protein. 
     
     
         3 . The method of microtubule inhibition of  claim 1 , wherein the DAT1 arrests mitosis thereby treating a disease where unwanted cell division is involved. 
     
     
         4 . The method of microtubule inhibition of  claim 1 , wherein the DAT1 inhibits microtubule polymerization thereby shifting tubulin-microtubule equilibrium. 
     
     
         5 . The method of microtubule inhibition of  claim 1 , wherein the DAT1 binds to tubulin with high affinity. 
     
     
         6 . The method of microtubule inhibition of  claim 5 , further comprising using fluorescence to bind the DAT1 to the tublin. 
     
     
         7 . The method of microtubule inhibition of  claim 2 , wherein the cancer cell is a HeLa cancer cell.