Method and System for Multiplex Genetic Analysis

Abstract

A method for preferentially localizing desired molecules within an optical confinement disposed upon a substrate is disclosed. The desired molecules are deposited over the surface of the substrate. The desired molecules that are not within the optical confinement are selectively removed from the surface of the substrate.

1 . A method of preferentially localizing desired molecules within an optical confinement disposed upon a substrate, comprising:
 depositing the desired molecules over the surface of the substrate; and   selectively removing the desired molecules from the surface of the substrate that is not within the optical confinement.   
     
     
         2 . A method of localizing a molecule within an optical confinement disposed on a substrate, comprising:
 providing a light activated coupling group on the surface of the substrate, including within the optical confinement;   directing activating radiation at the substrate, wherein the optical confinement permits activation radiation to be incident only within the optical confinement; and   coupling the molecule to the light activated coupling group.   
     
     
         3 . A method of selectively immobilizing a molecule of interest on a substrate, comprising:
 providing a substrate having a first surface component and a second surface component where the first and second surface component have different surface characteristics; and   selectively coupling the molecule of interest to the first surface component, based upon a difference between the surface characteristics of the first surface component and the surface characteristics of the second surface component.   
     
     
         4 . The method of  claim 3 , wherein first surface comprises a SiO 2  and the second surface comprises a metal or metal oxide. 
     
     
         5 . The method of  claim 3 , wherein the molecule of interest associates preferentially to the first surface component. 
     
     
         6 . The method of  claim 3 , wherein the substrate is contacted with an intermediate coupling group that is selective for associating with a surface having the surface characteristics of the first surface component. 
     
     
         7 . A method of selectively depositing a molecule of interest on selected regions of a substrate, comprising:
 providing a substrate having first and second components, the first component comprising a conductive material and the second component comprising an insulator; and   applying an electrical potential to the first component so as to increase or decrease association of a molecule of interest with a surface of the first component.