Method and System for Multiplex Genetic Analysis

Abstract

A method for selectively immobilizing a molecule of interest on a substrate is disclosed. A substrate having a first surface component and a second surface component is provided. The first and second surface components have different surface characteristics. The molecule of interest is selectively coupled to the first surface component based upon a difference between the surface characteristics of the first surface component and the surface characteristics of the second surface component.

1 . A method of selectively immobilizing a molecule of interest on a substrate, comprising:
 providing a substrate having a first surface component and a second surface component where the first and second surface component have different surface characteristics; and   selectively coupling the molecule of interest to the first surface component, based upon a difference between the surface characteristics of the first surface component and the surface characteristics of the second surface component.   
     
     
         2 . The method of  claim 1 , wherein the first surface component comprises SiO 2  and the second surface component comprises a metal or metal oxide. 
     
     
         3 . The method of  claim 2 , wherein the differing surface characteristics comprise surface charge. 
     
     
         4 . The method of  claim 1 , wherein the substrate comprises a layer of the second surface component on a layer of the first surface component, and wherein the substrate comprises a zero mode waveguide disposed through the surface component layer to the first surface component layer. 
     
     
         5 . The method of  claim 1 , wherein the molecule of interest associates preferentially to the first surface component. 
     
     
         6 . The method of  claim 1 , wherein the substrate is contacted with a first composition that selectively associates with the first surface component based upon the difference between the surface characteristics of the first and second surface components. 
     
     
         7 . The method of  claim 6 , wherein the first composition comprises a first coupling group, and wherein selectively coupling the molecule of interest to the first surface component comprises coupling the molecule of interest to the first coupling group. 
     
     
         8 . The method of  claim 7 , wherein the first coupling group comprises biotin. 
     
     
         9 . The method of  claim 1 , comprising contacting the substrate with a second composition that selectively associates with the second surface component based upon the difference between the surface characteristics of the first and second surface components. 
     
     
         10 . The method of  claim 9 , wherein the second composition comprises a second coupling group.