Combination of avian cell markers

Abstract

The present invention relates to a novel combination of avian cell markers which make it possible to characterize the said cells according to their phenotype, whether they are StX cells, stem cells or germ cells. The present invention also relates to a method for characterizing avian cells with the said markers, and to a method for culturing avian cells, in which the cells are characterized by the method according to the invention.

1 . Combination of markers allowing the characterization of avian cells of StX-type, stem cells or germ cells, including at least two markers chosen either from the same group or from two different groups:
 a. a marker of a target gene preferentially expressed in StX cells chosen from the following genes: 1P06, 2contig58, 60S-L14, ATM, Bloom syndrome, BTEB4, CD9, CHD helicase, Clock, cwf16 (FLJ10374), CXCR4, Dnmt2, enxl (ho-zeste 2), eomes, EWS, FGF-4, GATA-5, HOJ-1, N-AGN6P deacetylase, N-Cor1, NF2, p53, pml, rbm6, SA-2, SA-3, SARA, SCYE1, SEF, sf-1, SnoN, SOCS13, SSB-1, TC87479, T-cell APP 2C, TGF-beta2, WD40/FYVE-d protein 2, WD-RP3, Zan75, ZPC and combinations thereof, and/or   b. a marker of a target gene preferentially expressed in stem cells chosen from the following genes: 1P06, 1P08-A09, activin RIIB, astacin, Claudin-3, dapper-1, Dorfin, FPP synthase (fps), GalNAc-T3, gcnf, HSPb7, IRX4, LMX, pax-6, Slc38a2, sox-3, tra1 gp96, wnt-10a, wnt-11 and combinations thereof, and/or   c. a marker of a target gene preferentially expressed in germ cells chosen from the following genes: adiponectin, BMP-2IK, bruno like, CD34, CDK5 activator 1, dkk1, dkk3, DMRT1, emx2, endoglin, FAST-1, FGF R, FLJ00188, flk-1, gata-4, gcl, LHX9, NOS type III, plzf, PRL-R box1l, PTEN, SAMSN-1, slug, smad3, Smarcd3, sox-9, Strat8, TACC2, TC95408, TC97694, TGF RII, TGF RIII, tie2, tie-2, TR-alpha, VE-Cadherin, vera, Wisp-1 and combinations thereof.   
     
     
         2 . Combination according to  claim 1 , characterized in that the marker of a target gene preferentially expressed in StX cells is chosen from the markers of the following genes: 1P06, ATM, CXCR4, eomes, FGF-4, GATA-5, NF2, SOCS13, SSB-1, TC87479, T-cell APP 2C, TGF-beta2, WD-RP3, ZPC and combinations thereof. 
     
     
         3 . Combination according to one of  claims 1  or  2 , characterized in that it includes at least one marker of the ZPC gene. 
     
     
         4 . Combination according to one of  claims 1  to  3 , characterized in that the marker of a gene preferentially expressed in stem cells is chosen from the markers of the following genes: 1P06, activin RIIB, astacin, Claudin-3, dapper-1, FPP synthase (fps), GalNAc-T3, gcnf, LMX, pax-6, tra1 gp96, wnt-10a and combinations thereof. 
     
     
         5 . Combination according to one of  claims 1  to  4 , characterized in that it includes at least one marker of the 1P06 and tra-1 genes. 
     
     
         6 . Combination according to one of  claims 1  to  5 , characterized in that the marker of a target gene preferentially expressed in germ cells is chosen from the markers of the following genes: adiponectin, DMRT1, endoglin, FAST-1, FGF R, FLJ00188, gata-4, LHX9, plzf, PRL-R boxll, PTEN, Strat8, TGF RIII, Wisp-1 and combinations thereof. 
     
     
         7 . Combination according to one of  claims 1  to  6 , characterized in that it includes at least one marker of the dmrt-1 and vasa genes. 
     
     
         8 . Combination according to one of  claims 1  to  7 , characterized in that the markers are chosen from the antibodies able to bind specifically to the expression products of a target gene, mRNA, cDNA or polypeptide, or a fragment of these, and the nucleic acid fragments able to hybridize specifically with the mRNA expressed by said genes or the corresponding cDNA, or fragments of these. 
     
     
         9 . Combination according to one of  claims 1  to  8 , characterized in that it is assembled on a single support, preferably a standard support. 
     
     
         10 . Combination according to  claim 9 , characterized in that it is in the form of a DNA matrix, including a support on which the nucleic acid fragments susceptible to hybridize with the target genes are arranged, preferentially in a standard way. 
     
     
         11 . Combination according to  claim 10 , characterized in that it is arranged on a DNA chip. 
     
     
         12 . DNA chip, characterized in that it includes a combination of markers according to one of  claims 1  to  11 . 
     
     
         13 . Process for characterizing an avian cell including the analysis of the expression of genes expressed in said cell, using a combination of markers according to one of  claims 1  to  11  and the characterization of the phenotype of the analyzed cells. 
     
     
         14 . Process of growth of avian cells including the culture of cells in a suitable culture medium and the characterization of said cells by using a process according to  claim 13 . 
     
     
         15 . Process according to  claim 14 , characterized in that some cells are isolated, these cells being selected from StX cells, stem cells and germ cells. 
     
     
         16 . Process according to one of  claims 14  or  15 , characterized in that germ cells are obtained from StX cells, with the StX cells being cultivated in a suitable culture medium without the addition of an inactivated “feeder” layer.