Composition of Whey Growth Factor Extract for Reducing Muscle Inflammation

Abstract

The invention relates to the production of compositions containing milk products for use as a nutritional supplement. More specifically, it relates to compositions containing whey growth factor extract, as well as their use in methods for reducing muscle inflammation in individuals undertaking resistance exercise training. According to one aspect of the invention, there is provided the use of a composition comprising whey growth factor extract, isolated from a milk product by cation exchange chromatography, to reduce exercise-induced muscle inflammation.

1 . A muscle anti-inflammatory composition comprising whey growth factor extract, isolated from a milk product by cation exchange chromatography. 
   
   
       2 . A muscle anti-inflammatory composition comprising whey growth factor extract, isolated from a milk product by a process comprising the steps;
 a) applying the milk product to a SP Sepharose cation exchange column,   b) washing the column with a buffer of low ionic strength,   c) eluting the WGFE fraction with a buffer containing in the range 0.4-0.5M NaCl, or equivalent ionic strength, at pH 6.5.   
   
   
       3 . A muscle anti-inflammatory composition comprising whey growth factor extract, isolated from skim milk by a process comprising the steps;
 a) applying the skim milk to a SP Sepharose cation exchange column,   b) washing the column with a buffer of less than 0.008M NaCl,   c) eluting the WGFE fraction with a buffer containing 0.4M NaCl, at pH 6.5.   
   
   
       4 . The muscle anti-inflammatory composition as claimed in any one of  claims 1  to  3 , further comprising an additional protein source. 
   
   
       5 . The composition of  claim 4 , wherein the additional protein source is whey protein. 
   
   
       6 . The composition of  claim 5 , wherein the whey protein is whey protein isolate (WPI). 
   
   
       7 . The composition of  claim 6 , wherein the whey protein isolate comprises: 
     
       
         
               
               
               
             
                   
                   
               
                   
                 Moisture 
                 5.0% 
               
                   
                 Fat 
                 0.5% 
               
                   
                 pH (5% solution) 
                 6.3 
               
                   
                 Ash 
                 3.7% 
               
                   
                 Lactose 
                 0.5% 
               
                   
                 Protein (TN × 6.38) 
                 90.0% 
               
                   
                 Sodium 
                 0.7% 
               
                   
                 Phosphorous 
                 0.3% 
               
                   
                 Calcium 
                 0.15% 
               
                   
                   
               
           
              
             
             
              
              
              
              
              
              
              
              
              
              
             
          
         
       
     
   
   
       8 . The composition of any one of  claims 1  to  3 , wherein the whey growth factor extract is isolated from a source selected from the group consisting of cheese whey, rennet casein whey, acid casein whey and skim milk. 
   
   
       9 . The composition of  claim 8 , wherein the whey growth factor extract is isolated from skim milk. 
   
   
       10 . A composition according to any one of  claims 1  to  3 , when used as a muscle anti-inflammatory agent. 
   
   
       11 . A method of reducing muscle inflammation in a subject undertaking resistance exercise training comprising administering to the subject a composition comprising an effective amount of the muscle strength-enhancing composition of any one of  claims 1  to  3 . 
   
   
       12 . The method of  claim 11  wherein the composition is administered in a daily dose of at least 5 mg/kg body weight to 12.5 mg/kg body weight whey growth factor extract. 
   
   
       13 . The method of  claim 11 , wherein the composition comprises an additional protein source. 
   
   
       14 . A method of reducing muscle inflammation in a subject undertaking resistance exercise training comprising administering to the subject a composition comprising an effective amount of whey growth factor extract, isolated from a milk product by cation exchange chromatography, and an additional protein source taken separately. 
   
   
       15 . The method of  claim 13  or  14 , wherein the composition is administered in a daily dose of at least 5 mg/kg body weight to 12.5 mg/kg body weight whey growth factor extract, and at least 225 mg/kg body weight of additional protein source. 
   
   
       16 . The method of  claim 15  wherein the additional protein source is whey protein. 
   
   
       17 . The method of  claim 14 , wherein the administration to the subject is once per two or three days up to at least once per day. 
   
   
       18 . The method of  claim 14 , wherein the administration to the subject is before, and/or immediately after, resistance exercise training. 
   
   
       19 . The method of  claim 14 , wherein the administration is immediately after resistance exercise training. 
   
   
       20 . The method of  claim 18 , wherein the administration is between 20 minutes and two hours after exercise. 
   
   
       21 . A method of treating resistance exercise induced muscle inflammation in a subject, the method comprising administering to the subject whey growth factor extract, isolated from a milk product by cation exchange chromatography. 
   
   
       22 . A method of treating resistance exercise induced muscle inflammation in a subject, the method comprising administering to the subject whey growth factor extract, isolated from a milk product by a process according to  claim 2  or  3 . 
   
   
       23 . A method of treating resistance exercise induced muscle inflammation in a subject, the method comprising administering to the subject the composition of any one of  claims 1  to  3 . 
   
   
       24 . A food, drink, tablet or capsule comprising the composition of any one of  claims 1  to  3 . 
   
   
       25 . The food of  claim 24 , being in the form of a nutritional bar or snack food. 
   
   
       26 . A method of manufacturing a food, drink, tablet or capsule reduce muscle inflammation in a subject undertaking resistance exercise training the method comprising manufacturing the composition of any one of  claims 1  to  3 . 
   
   
       27 - 29 . (canceled)