US2009326239A1PendingUtilityA1

Fermentative preparation process for and crystal forms of cytostatics

Assignee: HOFMANN HANSPriority: Feb 19, 1998Filed: Mar 4, 2009Published: Dec 31, 2009
Est. expiryFeb 19, 2018(expired)· nominal 20-yr term from priority
A61P 43/00A61P 35/04A61P 35/00C07K 2299/00C12P 17/167C07D 493/04C07D 417/14C12P 17/181C12P 17/18C12R 2001/01C12N 1/205
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Claims

Abstract

The invention relates to a new process for concentrating epothilones in culture media, a new process for the production of epothilones, a new process for separating epothilones A and B and a new strain obtained by mutagenesis for the production of epothilones, as well as aspects related thereto. New crystal forms of epothilone B are also described.

Claims

exact text as granted — not AI-modified
1 . A method of separating epothilones from one another using chromatography on a reversed-phase column with an eluant containing a lower alkyl-cyanide. 
   
   
       2 . A method according to  claim 2 , herein a column material is used which is charged with hydrocarbon chains containing 18 carbon atoms, and the eluant used is a mixture of water and acetonitrile.

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