Nucleic Acid Sequences and Methods of Use for the Production of Plants with Modified Polyunsaturated Fatty Acids

Abstract

By this invention, novel nucleic acid sequences are provided, wherein said nucleic acid sequence is a genomic sequence of a plant desaturase encoding sequence. Also provided in the present invention are the promoter and intron sequences of the desaturase genomic sequences. Furthermore, recombinant DNA constructs employing the polynucleotide sequences are provided. The instant invention also provides methods for the modification of fatty acid compositions in host plant cells.

1 - 89 . (canceled) 
     
     
         90 . A canola plant having a nucleic acid molecule comprising a promoter functional in a host plant cell operably linked to a polynucleotide that is a FAD3 intron or complement thereof or fragment of either, and a transcriptional termination region functional in said host plant cell, wherein a seed of said canola plant exhibits a modified fatty acid composition that is about 80-85% oleic acid, about 1-2% linoleic acid, and about 1-3% linolenic acid. 
     
     
         91 - 102 . (canceled) 
     
     
         103 . The canola plant according to  claim 90 , wherein said polynucleotide has at least 80% identity to a sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 29, complements thereof, and fragments of either. 
     
     
         104 . The canola plant according to  claim 90 , wherein said polynucleotide has at least 90% identity to a sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 29, complements thereof, and fragments of either. 
     
     
         105 . The canola plant according to  claim 90 , wherein said polynucleotide has at least 95% identity to a sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 29, complements thereof, and fragments of either. 
     
     
         106 . The canola plant according to  claim 90 , wherein said polynucleotide has at least 97% identity to a sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 29, complements thereof, and fragments of either. 
     
     
         107 . The canola plant according to  claim 90 , wherein said polynucleotide has at least 98% identity to a sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 29, complements thereof, and fragments of either. 
     
     
         108 . The canola plant according to  claim 90 , wherein said polynucleotide has at least 99% identity to a sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 29, complements thereof, and fragments of either. 
     
     
         109 . The canola plant according to  claim 90 , wherein said polynucleotide is selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 29, complements thereof, and fragments of either. 
     
     
         110 . The canola plant according to  claim 90 , wherein said promoter is a heterologous promoter.