US2010009442A1PendingUtilityA1

Stem Cell Culture Medium and Method

57
Assignee: SASAI YOSHIKIPriority: Sep 22, 2006Filed: Sep 24, 2007Published: Jan 14, 2010
Est. expirySep 22, 2026(~0.2 yrs left)· nominal 20-yr term from priority
Inventors:Yoshiki SasaiKiichi Watanabe
C12N 2501/999C12N 5/0606C12N 2501/70C12N 5/0603C12N 2506/00
57
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Claims

Abstract

Stem cells such as embryonic stem cells (ES cells), including human ES cells, are cultured in a medium comprising a ROCK inhibitor, and a stem cell culture medium, optionally serum free, comprises a ROCK inhibitor.

Claims

exact text as granted — not AI-modified
1 . A method of culturing stem cells, which comprises treating the stem cells with a ROCK (Rho-kinase) inhibitor. 
   
   
       2 . The method of  claim 1 , wherein the stem cells are pluripotent stem cells. 
   
   
       3 . The method of  claim 1 , wherein the stem cells are primate stem cells. 
   
   
       4 . The method of  claim 3 , wherein the stem cells are human stem cells. 
   
   
       5 . The method of  claim 1 , wherein the stem cells are dissociated. 
   
   
       6 . The method of  claim 5 , wherein the dissociated stem cells are single stem cells or aggregated stem cells. 
   
   
       7 . The method of  claim 1 , which further comprises dissociating the stem cells. 
   
   
       8 . The method of  claim 7 , wherein the stem cells are treated with a ROCK inhibitor before the dissociation of the stem cells. 
   
   
       9 . The method of  claim 7 , wherein the stem cells are treated with a ROCK inhibitor after the dissociation of the stem cells. 
   
   
       10 . The method of  claim 1 , wherein the ROCK inhibitor is Y-27632, Fasudil, or H-1152. 
   
   
       11 .- 16 . (canceled) 
   
   
       17 . A cell preparation comprising a stem cell and a ROCK inhibitor. 
   
   
       18 . The cell preparation of  claim 17 , wherein the stem cell is dissociated. 
   
   
       19 . A stem cell culture medium comprising a ROCK inhibitor. 
   
   
       20 . The medium of  claim 19 , comprising a basal medium and a ROCK inhibitor. 
   
   
       21 . The medium of  claim 19 , which is serum-free. 
   
   
       22 .- 60 . (canceled) 
   
   
       61 . The cell preparation of  claim 17 , wherein the stem cell is pluripotent. 
   
   
       62 . The medium of  claim 20 , further comprising an iron transporter. 
   
   
       63 . The medium of  claim 19 , for pluripotent cells. 
   
   
       64 . The medium of  claim 19 , for human cells.

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