Method for producing a composition for promoting survival of transplanted hematopoietic stem cell

Abstract

HLA matched activated lymphocytes in mononuclear cells separated from peripheral blood or umbilical cord blood are proliferated and activated. After separating and collecting, the HLA matched activated lymphocytes are employed as the main component of a composition for promoting survival of transplanted hematopoietic stem cells. The obtained composition is widely usable in, for instance, prevention of survival failure of transplanted hematopoietic stem cells and therapy for promoting the survival thereof. Although the dose of the composition varies depending on the age, conditions, etc. of a patient, a humanized antibody is administered in a dose of from 0.2 to 20 ml/kg/day to mammals including humans. The composition is administered by intravenous injection either once a day (single administration or continuous administration) or intermittently once to 3 times in a week or once in 2 or 3 weeks.

1 . A method for producing a composition for promoting survival after hematopoietic stem cell transplantation, comprising a process for separating mononuclear cells from peripheral blood or umbilical cord blood containing HLA matched lymphocytes; a process for proliferating and activating HLA matched lymphocytes in said separated mononuclear cells; and a process for separating said proliferating and activating HLA matched lymphocytes and preparing them to a formulation in which they constitute a main component. 
   
   
       2 . A method for producing a composition for promoting survival after hematopoietic stem cell transplantation according to  claim 1 , wherein proliferation and activation of said HLA matched lymphocytes are carried out in any one of interleukin 2 or anti-CD3 antibody or in combination of them. 
   
   
       3 . A method for producing a composition for promoting survival after hematopoietic stem-cell transplantation according to  claim 1  or  2 , wherein various kinds of cytokines or mitogens are added to medium for proliferation and activation of said HLA matched lymphocytes.