Test strip for detecting gastric problems and detecting method thereof

Abstract

The present invention provides a test strip and a detecting method thereof. The test strip comprises a first sheet, a second sheet a first filter and a second filter. The first sheet comprises a bulge portion deposited substantially in the center thereof. The second sheet comprises a depression in alignment with the bulge portion to form a sealing space. The first filter is disk-shaped and deposited in the sealing space. The first filter is used to receive a biological sample. The second filter is ring shaped and disposed in the sealing space and under the first filter to receive the biological sample coming from the first filter. The first filter comprises sodium azide, sodium dihydrogen phosphate, and urea, and the second filter comprises sodium azide, phenol red, and urea.

1 . A test strip for detecting  Helicobacter pylori  comprising:
 a first sheet comprising a bulge portion deposited substantially in the center thereof,   a second sheet comprising a depression in alignment with said bulge portion to form a sealing space,   a first filter to receive a biological sample, said first filter comprising a disk configuration and being deposited in said sealing space,   the test strip being characterized in that:   said test strip further comprising a second filter with a ring configuration which is disposed in said sealing space and under said first filter to receive the biological sample coming from said first filter,   wherein said first filter comprises sodium azide, sodium dihydrogen phosphate, and urea, and   said second filter comprises sodium azide, phenol red, and urea.   
   
   
       2 . The test strip of  claim 1 , wherein said sodium azide has a volumetric concentration ranging from about 0.001% to about 0.03%. 
   
   
       3 . The test strip of  claim 1 , wherein said phenol red has volumetric concentration ranging from about 0.025% to about 0.075%. 
   
   
       4 . The test strip of  claim 1 , wherein said sodium dihydrogen phosphate has a concentration ranging from about 6.5 mM to about 19.5 mM. 
   
   
       5 . The test strip of  claim 1 , further comprising a membrane disposed between said first filter and said second filter. 
   
   
       6 . The test strip of  claim 5 , wherein said sodium azide has a volumetric concentration ranging from about 0.001% to about 0.03%, said phenol red has volumetric concentration ranging from about 0.025% to about 0.075%, said sodium dihydrogen phosphate has a concentration ranging from about 6.5 mM to about 19.5 mM, said urea has a volumetric concentration ranging from about 0.5% to about 1%, and said phenol red has volumetric concentration ranging from about 0.025% or 0.075%. 
   
   
       7 . A method to detect  Helicobacter pylori  which is characterized in comprising the following steps:
 providing a test strip comprising a first sheet, a second sheet, a first filter and a second filter, wherein said first sheet has a bulge portion disposed substantially in the center thereof, said second sheet has a depression in alignment with said bulge portion to form a sealing space, said first filter is in a disk configuration and comprises sodium azide, sodium dihydrogen phosphate and urea, and said second filter is in a ring configuration and comprises sodium azide, phenol red and urea, said second filter being disposed in said sealing space under said first filter,   providing a biological sample,   peeling said first sheet and applying said biological sample on said first filter,   sealing said test strip, wherein said bulge portion squeezes said first filter and said second filter to achieve a better sealing effect, and   comparing the difference between the colors of said first filter and said second filter to determine whether said sample has  Helicobacter pylori  or not.   
   
   
       8 . The method of  claim 7 , wherein said sodium azide has a volumetric concentration ranging from about 0.001% to about 0.03%. 
   
   
       9 . The method of  claim 7 , wherein said phenol red has volumetric concentration ranging from about 0.025% to about 0.075%. 
   
   
       10 . The method of  claim 7 , wherein said sodium dihydrogen phosphate has a concentration ranging from about 6.5 mM to about 19.5 mM. 
   
   
       11 . The method of  claim 7 , wherein said test strip further comprises a membrane disposed between said first filter and said second filter. 
   
   
       12 . The method of  claim 11 , wherein said sodium azide has a volumetric concentration ranging from about 0.001% to about 0.03%, said phenol red has volumetric concentration ranging from about 0.025% to about 0.075%, said sodium dihydrogen phosphate has a concentration ranging from about 6.5 mM to about 19.5 mM, said urea has a volumetric concentration ranging from about 0.5% to about 1%, and said phenol red has volumetric concentration ranging from about 0.025% or 0.075%.