Medium for the Production of Tiacumicin B

Abstract

Methods, processes and materials for the production and recovery of Tiacumicins produced by culturing a microorganism belonging to the species Dactylosporangium aurantiacum subspecies hamdenensis having the ability to produce and accumulate one or more Tiacumicin in a nutrient medium comprising a carbon source, a nitrogen source, trace elements such as inorganic salts, and an adsorbent, wherein said nitrogen source comprises fish powder, and wherein said Tiacumicin is produced in a yield greater than about 50 mg/L broth.

1 - 16 . (canceled) 
   
   
       17 . A medium for the production of tiacumicin B, said medium comprising a microorganism capable of producing tiacumicin B, a carbon source, a nitrogen source, trace elements, and an adsorbent resin capable of adsorbing the tiacumicin B. 
   
   
       18 - 21 . (canceled) 
   
   
       22 . The nutrient medium according to  claim 17  wherein said nitrogen source is fish powder. 
   
   
       23 . The medium according to  claim 17  wherein said microorganism is  Dactylosporangium aurantiacum  NRRL 18085. 
   
   
       24 - 27 . (canceled) 
   
   
       28 . The nutrient medium according to  claim 17  wherein the nutrient medium comprises 0.5%-15% by weight of the adsorbent resin. 
   
   
       29 . The nutrient medium according to  claim 17  wherein the adsorbent resin is selected from the group consisting of Amberlite® XAD16, XAD16HP, XAD2, XAD7HP, XAD1180, XAD1600, IRC50, Duolite® XAD761 and a reverse phase silica gel. 
   
   
       30 . The nutrient medium according to  claim 17  wherein the trace elements are inorganic salts. 
   
   
       31 . The nutrient medium according to  claim 17  wherein the nutrient medium comprises 0.2-10% by weight of the carbon source. 
   
   
       32 . The nutrient medium according to  claim 17  wherein the nutrient medium comprises 0.1-5.0% by weight of the nitrogen source. 
   
   
       33 . The nutrient medium according to  claim 17  wherein the nutrient medium comprises 0.02-2.0% by weight of the trace elements.