Nucleic acid molecules and other molecules associated with the tetrapyrrole pathway

Abstract

The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, nucleic acid sequences from maize and soybean associated with the tetrapyrrole pathway. The invention encompasses nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression and transgenic plants.

1 - 9 . (canceled) 
     
     
         10 . A transformed plant comprising a nucleic acid molecule which comprises:
 (a) an exogenous promoter region which functions in a plant cell to cause the production of an mRNA molecule; which is linked to;   (b) a structural nucleic acid molecule, wherein said structural nucleic acid molecule comprises a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof, or which is linked to   (c) a 3′ non-translated sequence that functions in said plant cell to cause the termination of transcription and the addition of polyadenylated ribonucleotides to said 3′ end of said mRNA molecule.   
     
     
         11 . The transformed plant according to  claim 10 , wherein said nucleic acid sequence is the complement of a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677. 
     
     
         12 . The transformed plant according to  claim 10 , wherein said nucleic acid sequence is in the antisense orientation of a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677. 
     
     
         13 . The transformed plant according to  claim 10 , wherein said nucleic acid sequence shares between 100% and 95% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof. 
     
     
         14 . The transformed plant according to  claim 13 , wherein said nucleic acid sequence shares between 100% and 98% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof. 
     
     
         15 . The transformed plant according to  claim 14 , wherein said nucleic acid sequence shares between 100% and 99% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof. 
     
     
         16 . The transformed plant according to  claim 15 , wherein said nucleic acid sequence shares 100% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof. 
     
     
         17 . A transformed seed comprising a transformed plant cell comprising a nucleic acid molecule which comprises:
 (a) an exogenous promoter region which functions in said plant cell to cause the production of an mRNA molecule; which is linked to;   (b) a structural nucleic acid molecule, wherein said structural nucleic acid molecule comprises a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof, which is linked to   (c) a 3′ non-translated sequence that functions in said plant cell to cause the termination of transcription and the addition of polyadenylated ribonucleotides to said 3′ end of said mRNA molecule.   
     
     
         18 . The transformed seed according to  claim 17 , wherein said nucleic acid sequence is the complement of a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677. 
     
     
         19 . The transformed seed according to  claim 17 , wherein said exogenous promoter region functions in a seed cell. 
     
     
         20 . The transformed seed according to  claim 17 , wherein said nucleic acid sequence shares between 100% and 95% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof. 
     
     
         21 . The transformed seed according to  claim 20 , wherein said nucleic acid sequence shares between 100% and 98% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof. 
     
     
         22 . The transformed seed according to  claim 21 , wherein said nucleic acid sequence shares between 100% and 99% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof. 
     
     
         23 . The transformed seed according to  claim 22 , wherein said nucleic acid sequence shares 100% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof. 
     
     
         24 . A method of growing a transgenic plant comprising
 (a) planting a transformed seed comprising a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof, and   (b) growing a plant from said seed.   
     
     
         25 . A substantially purified nucleic acid molecule comprising a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 677 and complements thereof. 
     
     
         26 . The substantially purified nucleic acid molecule of  claim 25 , wherein said nucleic acid molecule encodes a maize protein or fragment thereof. 
     
     
         27 . The substantially purified nucleic acid molecule of  claim 25 , wherein said nucleic acid molecule encodes a soybean protein or fragment thereof.