Detection of Analytes Using Reorganization Energy

Abstract

The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process.

1 . A method of detecting a target analyte in a test sample comprising
 a) adding a target analyte to an electrode comprising
 i) a solvent accessible transition metal complex comprising a metal selected from the group consisting of manganese, technetium, rhenium, iron, ruthenium, osmium, cobalt, rhodium, iridium, nickel, palladium, platinum, copper, silver and gold; and 
 ii) a binding ligand that will bind said target analyte; 
   such that upon binding of said target analyte to said binding ligand, a solvent inhibited transition metal complex is formed; and   b) measuring a change in E 0  of said transition metal complex.   
     
     
         2 . A method according to  claim 1 , wherein said measuring comprises applying at least a first input signal to said solvent inhibited transition metal complex and receiving an output signal. 
     
     
         3 . A method according to  claim 2 , wherein in the absence of target analyte, said first input signal does not result in a significant change in E 0 . 
     
     
         4 . A method according to  claim 2 , wherein said first input signal comprises at least an AC component. 
     
     
         5 . A method according to  claim 2 , further comprising applying input signal at a plurality of frequencies. 
     
     
         6 . A method according to  claim 2  or  4 , wherein said input signal comprises at least a DC component. 
     
     
         7 . A method according to  claim 6 , further comprising applying input signal at a plurality of voltages. 
     
     
         8 . A method according to  claim 2 , wherein said output signal is a current. 
     
     
         9 . A method according to  claim 8 , wherein said current is an AC current. 
     
     
         10 . A method according to  claim 1  wherein said electrode further comprises gold. 
     
     
         11 . A method according to  claim 1  wherein said electrode further comprises graphite-pyrene. 
     
     
         12 . A method according to  claim 1  wherein said electrode further comprises a self-assembled monolayer. 
     
     
         13 . A method according to  claim 12  wherein said self assembled monolayer comprises conductive oligomers. 
     
     
         14 . A method according to  claim 1 , wherein said solvent accessible transition metal complex has at least one coordination site occupied by a polar coordination group. 
     
     
         15 . A method according to  claim 1 , wherein said solvent accessible transition metal complex has at least one coordination site occupied by a water molecule. 
     
     
         16 . A method according to  claim 1 , wherein said solvent accessible transition metal complex has at least two coordination sites occupied by a polar coordination group. 
     
     
         17 . A method according to  claim 1 , wherein said binding ligand is covalently attached to said solvent accessible transition metal complex. 
     
     
         18 . A method according to  claim 1 , wherein said binding ligand is covalently attached to said electrode. 
     
     
         19 . A method according to  claim 1 , wherein said solvent accessible transition metal complex is covalently attached to said electrode. 
     
     
         20 . A method according to  claim 18 , wherein said covalent attachment is via a spacer. 
     
     
         21 . A method according to  claim 19 , wherein said covalent attachment is via a spacer. 
     
     
         22 . A method according to  claim 20  or  21 , wherein said spacer is a conductive oligomer.