Simulated blood components and methods

Abstract

A control and a cellular fraction for use therein and methods for making the same, including a first cellular component including a plurality of a first group of processed animal red blood cells other than human blood cells; a second cellular component including a plurality of a second group of processed animal red blood cells other than human blood cells; a third cellular component including a plurality of a third group of processed animal red blood cells other than human blood cells; wherein one or more of the first second and third cellular components include a nucleus; and the control simulates erythroblasts of a human blood sample on an automated blood analyzer that analyzes blood cells using impedance, optical measurements or both.

1 . (canceled) 
   
   
       2 . A stand-alone erythroblast control, comprising:
 a first cellular component including a plurality of a first group of processed animal red blood cells other than human blood cells; and   a second cellular component including a plurality of a second group of processed animal red blood cells other than human blood cells;   wherein upon passing through an automated hematology analyzer the first group of processed animal red blood cells are detected by the analyzer as white blood cells but are not detected as neutrophils, eosinophils, basophils, lymphocytes or monocytes so that the presence of the first cellular component allows for the second group of processed animal red blood cells to be detected as an erythroblast on an automated blood analyzer that analyzes blood cells using impedance, optical measurements or both.   
   
   
       3 - 20 . (canceled) 
   
   
       21 . The control of  claim 2 , wherein the second group of processed animal red blood cells will not be detected as an erythroblast on an automated blood analyzer if the first group of processed animal red blood cells is not present in the control. 
   
   
       22 . The control of  claim 2 , wherein one or more of the first or second cellular components is a processed red blood cell derived from alligators, turkeys, geese, chickens, sharks, cows, pigs, goats, salmon, trout, or another source of a blood cell with a nucleus. 
   
   
       23 . The control of  claim 2 , wherein cells from the second cellular component function to simulate erythroblasts of a human blood sample on an automated blood analyzer that analyzes blood cells using impedance, optical measurements or both. 
   
   
       24 . The control of  claim 21 , wherein upon passing through an automated hematology analyzer cells of the first and second cellular components are detected by the analyzer as a white blood cell, and cells of the third cellular component are detected as an erythroblast. 
   
   
       25 . The control of  claim 2 , wherein at least one of the first or second cellular components has a size less than about 450 fL. 
   
   
       26 . The control of  claim 2 , wherein at least one of the first or second cellular components is a processed red blood cell derived from alligators. 
   
   
       27 . The control of  claim 2 , wherein at least one cellular component includes cells that have an overall average cell size ranging from about 300 to about 650 fl. 
   
   
       28 . The control of  claim 2 , wherein the at least one cellular component of the second part include cells that have an overall average cell size ranging from about 425 to about 575 fl. 
   
   
       29 . The control of  claim 2 , wherein at least one cellular component includes a plurality of alligator red blood cells that have been processed in a hypertonic solution for altering its volume relative to their original cell volume.