Compositions and methods for isolation, propagation, and differentiation of human stem cells and uses thereof

Abstract

The invention is directed to the field of human stem cells and includes methods and compositions for isolating, propagating, and differentiating human stem cells. The invention provides therapeutic uses of the methods and compositions, including autologous transplantation of treated cells into humans for treatment of Parkinson's and other neuronal disorders.

1 - 30 . (canceled) 
   
   
       31 . A cell culture medium for differentiating central nervous system (CNS) stem cells comprising RA, dBcAMP, FGF8, and GDNF. 
   
   
       32 . The cell culture medium of claim  30  comprising 10 −6  M RA, 1 mM dBcAMP, 20 ng/mi FGF8 and 20 ng/ml GDNF. 
   
   
       33 . A method of causing central nervous system stem cells to differentiate in vitro comprising culturing CNS stem cells in the cell culture medium of claim  30 . 
   
   
       34 . The cell culture medium of  claim 31  comprising about 10 −8  to about 10 −4  M RA, about 0.01 to about 3 mM dBcAMP, about 0.02 to about 200 ng/ml FGF8, and about 0.02 to about 200 ng/ml GDNF.