BRACHYSPIRA PILOSICOLI 72kDa OUTER-MEMBRANE PROTEIN AND DIAGNOSTIC AND THERAPEUTIC USES THEREOF

Abstract

The invention relates to Brachyspira pilosicoli 72 kDa outer-membrane protein (Bpmp-72) and its amino acid and nucleotide sequence. The invention also relates to the uses of these sequences in prophylactic (vaccination) and therapeutic treatment of infections with Brachyspira pilosicoli (internal spirochaetosis).

1 - 26 . (canceled) 
     
     
         27 . An isolated polypeptide comprising an amino acid sequence set forth in SEQ ID NO:2 or analogue thereof having at least 60% homology thereto, wherein the isolated polypeptide is capable of eliciting an immune response to  Brachyspira pilosicoli , and wherein the isolated polypeptide is capable of eliciting an antibody that specifically binds to the 34 kDa C-terminal portion of the Bpmp-72 outer membrane protein that consists of the amino acid sequence set forth in SEQ ID NO:2. 
     
     
         28 . The isolated polypeptide of  claim 27 , wherein the analogue has at least 70% homology to the amino acid sequence set forth in SEQ ID NO:2. 
     
     
         29 . The isolated polypeptide of  claim 27 , wherein the analogue has at least 80% homology to the amino acid sequence set forth in SEQ ID NO:2. 
     
     
         30 . The isolated polypeptide of  claim 27 , wherein the analogue has at least 90% homology to the amino acid sequence set forth in SEQ ID NO:2. 
     
     
         31 . The isolated polypeptide of  claim 27 , wherein the analogue has at least 95% homology to the amino acid sequence set forth in SEQ ID NO:2. 
     
     
         32 . The isolated polypeptide of  claim 27 , wherein the analogue has at least 98% homology to the amino acid sequence set forth in SEQ ID NO:2. 
     
     
         33 . The isolated polypeptide of  claim 27 , wherein the analogue has an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:1, SEQ ID NO: 12, SEQ ID NO:13, SEQ ID NO: 14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO: 17, SEQ ID NO:8, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO:22. 
     
     
         34 . A polynucleotide encoding the isolated polypeptide of  claim 27 . 
     
     
         35 . A vector comprising the polynucleotide of  claim 34 . 
     
     
         36 . A pharmaceutical composition comprising the isolated polypeptide of  claim 27  and a pharmaceutically acceptable carrier, diluent, or adjuvant. 
     
     
         37 . A vaccine composition comprising the isolated polypeptide of  claim 27  and a pharmaceutically acceptable carrier, diluent, or adjuvant. 
     
     
         38 . A method of immunizing a subject against infection with Brachyspira pilosicoli comprising administering the vaccine composition of  claim 37  to a subject in need thereof.