Novel Nucleotide and Amino Acid Sequences, and Assays and Methods of Use Thereof for Diagnosis of Lung Cancer

Abstract

Novel markers for lung cancer that are both sensitive and accurate. These markers are overexpressed in lung cancer specifically, as opposed to normal lung tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis of lung cancer. The markers of the present invention, alone or in combination, show a high degree of differential detection between lung cancer and non-cancerous states.

1 - 23 . (canceled) 
     
     
         24 . An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 1795. 
     
     
         25 . The polypeptide of  claim 24 , wherein said polypeptide consists of the amino acid sequence set forth in SEQ ID NO: 1795. 
     
     
         26 . An isolated polypeptide comprising an amino acid sequence at least 95% identical to SEQ ID NO:1300. 
     
     
         27 . An isolated polypeptide consisting of the amino acid sequence of SEQ ID NO: 1793. 
     
     
         28 . A monoclonal or polyclonal antibody that specifically binds to an epitope in a polypeptide of  claim 24 , or an epitope-binding fragment thereof. 
     
     
         29 . The antibody of  claim 28  that specifically binds to an epitope in a polypeptide consisting of the amino acid sequence of any one of SEQ ID NOs: 1300 or 1795. 
     
     
         30 . A kit for detecting lung cancer, comprising the antibody of  claim 28 . 
     
     
         31 . The kit of  claim 30 , wherein said kit further comprises at least one immunoassay reagent. 
     
     
         32 . The kit of  claim 31 , wherein said immunoassay reagent is selected from the group consisting of an enzyme linked immunosorbent assay (ELISA), an immunoprecipitation assay, an immunofluorescence analysis, an enzyme immunoassay (EIA), a radioimmunoassay (RIA), and a Western blot analysis. 
     
     
         33 . A method for detecting lung cancer, comprising detecting overexpression of the polypeptide comprising the polypeptide sequence with the amino acid sequence of SEQ ID NOs: 1300 or 1795 in a sample. 
     
     
         34 . The method of  claim 33 , wherein detecting cancer comprises detecting the presence or severity of the cancer, prognosis, prediction, screening, early diagnosis, staging, treatment selection, treatment monitoring. 
     
     
         35 . A biomarker for detecting lung cancer comprising a polypeptide with the amino acid sequence of  claim 25  marked with a label.