Mammalian chemokines; receptors; reagents; uses

Abstract

Novel chemokines and 7 transmembrane receptors from mammals, reagents related thereto, including purified proteins, specific antibodies, and nucleic acids encoding the chemokines and receptors are disclosed. Methods of using the chemokines, receptors, reagents and diagnostic kits are also provided.

1 . An isolated polynucleotide that hybridizes to a polynucleotide comprising the nucleotide sequence set forth in SEQ ID NO: 9 under stringent conditions comprising a hybridization temperature of 55° C. or more and a salt concentration of 500 mM or less. 
     
     
         2 . The polynucleotide of  claim 1  which comprises at least 80% sequence identity to the nucleotide sequence set forth in SEQ ID NO: 9. 
     
     
         3 . The polynucleotide of  claim 1  encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 10. 
     
     
         4 . The polynucleotide of  claim 1  comprising the nucleotide sequence set forth in SEQ ID NO: 9. 
     
     
         5 . The polynucleotide of  claim 1  which is detectably labeled. 
     
     
         6 . The polynucleotide of  claim 1  fused to a polynucleotide encoding a heterologous protein. 
     
     
         7 . The polynucleotide of  claim 6  wherein the heterologous protein is a member selected from the group consisting of luciferase, bacterial beta-galactosidase, trpE, Protein A, beta-lactamase, alpha amylase, alcohol dehydrogenase, a FLAG tag, and yeast alpha mating factor. 
     
     
         8 . An isolated host cell comprising the polynucleotide of  claim 1 . 
     
     
         9 . The host cell of  claim 8  which is  E. coli, B. subtilis,  a yeast cell,  S. cerevisiae, Pichia, Dictyostelium,  or an animal cell. 
     
     
         10 . An isolated vector comprising the polynucleotide of  claim 1 . 
     
     
         11 . The vector of  claim 10  which is a plasmid. 
     
     
         12 . The vector of  claim 11  comprising control elements that are operably linked to said polynucleotide whereby a coding sequence within said polynucleotide can be transcribed and translated in a host cell, and at least one of said control elements is heterologous to said coding sequence. 
     
     
         13 . A method for making a polypeptide encoded by the polynucleotide of  claim 1  comprising introducing the polynucleotide into a host cell under conditions whereby the polypeptide is expressed.