Nucleic acid encoding receptor type protein kinase

Abstract

To provide a nucleic acid encoding a receptor protein kinase, wherein the nucleic acid has tandem duplication in a nucleotide sequence of a juxtamembrane and is useful for diagnosis of leukemia; a polypeptide encoded by the nucleic acid; an antibody capable of specifically binding to a region encoded by the nucleic acid having tandem duplication occurring in a nucleotide sequence of a juxtamembrane; a nucleic acid capable of specifically binding to the nucleic acid having tandem duplication occurring in a nucleotide sequence of juxtamembrane; a method for detection of the nucleic acid encoding a receptor protein kinase; and a kit therefor. A nucleic acid encoding a receptor protein kinase, wherein the nucleic acid has tandem duplication in a nucleotide sequence of a juxtamembrane; a polypeptide encoded by the nucleic acid; an antibody capable of specifically binding to the portion of the polypeptide; a nucleic acid capable of specifically binding to the nucleic acid; a method for detection of the nucleic acid; and a kit for detection.

1 . A primer set composition for amplifying at least a portion of a region of nucleic acid that encodes a polypeptide having FLT3 kinase activity, which comprises:
 at least two isolated nucleic acid oligomers that, in a nucleic acid amplification reaction, will prime the amplification of at least one nucleic acid fragment located within the nucleic acid fragment located within the nucleic acid region encoding FLT3 kinase that is defined by SEQ ID NO: 26 and SEQ ID NO: 33.   
     
     
         2 . The primer set composition of  claim 1  wherein the nucleic acid fragment is located within the nucleic acid region encoding FLT3 kinase that is defined by SEQ ID NO: 26 and SEQ ID NO: 27. 
     
     
         3 . The primer set composition of  claim 1  wherein the nucleic acid fragment is located within the nucleic acid region encoding FLT3 kinase that is defined by SEQ ID NO: 30 and SEQ ID NO: 31. 
     
     
         4 . The primer set composition of  claim 1  wherein the nucleic acid fragment is located within the nucleic acid region encoding FLT3 kinase that is defined by SEQ ID NO: 32 and SEQ ID NO: 33. 
     
     
         5 . A nucleic acid fragment composition for detecting myelodysplastic syndrome (MDS) or leukemia in a subject, which comprises:
 at least one isolated nucleic acid fragment derived from the nucleic acid region encoding FLT3 kinase that is defined by SEQ ID NO: 26 and SEQ ID NO: 33 and which comprises at least one mutation compared to the wild-type nucleic acid sequence encoding FLT3 kinase.   
     
     
         6 . The nucleic acid fragment composition of  claim 5  wherein the nucleic acid fragment is derived from the nucleic acid region encoding FLT3 kinase that is defined by SEQ ID NO: 26 and SEQ ID NO: 27. 
     
     
         7 . The nucleic acid fragment composition of  claim 5  wherein the nucleic acid fragment is derived from the nucleic acid region encoding FLT3 kinase that is defined by SEQ ID NO: 30 and SEQ ID NO: 31. 
     
     
         8 . The nucleic acid fragment composition of  claim 5  wherein the nucleic acid fragment is derived from the nucleic acid region encoding FLT3 kinase that is defined by SEQ ID NO: 32 and SEQ ID NO: 33.