Multi-well plate and method of manufacture

Abstract

A method of manufacture and assembly of multiwell plates employing targeted radiation at an interface in order to achieve bonding is disclosed. The method accommodates glass and polymer attachment as well as polymer to polymer attachment. Resultant plates have unique flatness and optical properties.

1 .- 31 . (canceled) 
     
     
         32 . A multiwell plate for use in assaying samples compromising in combination:
 an upper plate having at least one well therein extending between open ends on opposite surfaces of said upper plate;
 a lower plate of polymer film having a thickness less than 5 mils and bonded to said upper plate by means other than adhesive attachment; and 
 whereby said lower plate extends across the at least one well creating a well bottom having a top surface. 
   
     
     
         33 . The multiwell plate of  claim 32  wherein said top surface of said well bottom has a flatness of less than 10 microns as measured across the diameter of said well. 
     
     
         34 . The multiwell plate of  claim 32  wherein said lower plate forms well bottoms for a matrix of wells, said lower plate having bottom surface having a flatness of no greater than 50 microns when measured across said entire bottom surface on a line intersecting the diameters of each said well bottom in said line. 
     
     
         35 . The multiwell plate of  claim 32  wherein said lower plate is porous. 
     
     
         36 . The multiwell plate of  claim 32  wherein said top surface of said at least one well comprises
 reactive coating and wherein sidewalls of said at least one well are free of the reactive coating.   
     
     
         37 .- 50 . (canceled)