US2010129870A1PendingUtilityA1

Method for obtaining oligonucleotide

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Assignee: NEC SOFTWARE LTDPriority: Aug 3, 2006Filed: Jul 10, 2007Published: May 27, 2010
Est. expiryAug 3, 2026(~0.1 yrs left)· nominal 20-yr term from priority
Inventors:Fumiko NishikawaSatoshi NishikawaMakio FuruichiHiroshi MizunoIwao Waga
C12N 2310/16C12N 15/115C12N 2330/30
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Claims

Abstract

The present invention is to provide a method for obtaining an oligonucleotide such as RNA aptamer having high binding capacity to a target substance with easy-to-use and high purity. The method for obtaining oligonucleotide according to the present invention is as follows: A method for obtaining oligonucleotide comprising the steps of: performing an electrophoresis of a nucleic acid molecule/target substance complex comprising a nucleic acid molecule and a target substance; recovering said nucleic acid molecule/target substance complex; extracting the nucleic acid molecule from said nucleic acid molecule/target substance complex; gene amplifying said nucleic acid molecule.

Claims

exact text as granted — not AI-modified
1 . A method for obtaining oligonucleotide comprising the steps of:
 performing an electrophoresis of a nucleic acid molecule/target substance complex comprising a nucleic acid molecule and a target substance;   recovering said nucleic acid molecule/target substance complex;   extracting the nucleic acid molecule from said nucleic acid molecule/target substance complex;   gene amplifying said nucleic acid molecule.   
     
     
         2 . The method for obtaining oligonucleotide according to  claim 1 , wherein said step of performing the electrophoresis is a step of performing an electrophoresis at least using an agarose gel. 
     
     
         3 . The method for obtaining oligonucleotide according to  claim 1 , wherein said method for obtaining oligonucleotide comprises the step of performing the electrophoresis using the agarose gel and the step of performing the electrophoresis using a polyacrylamide gel. 
     
     
         4 . The method for obtaining oligonucleotide according to  claim 1 , further comprising a step of contacting said nucleic acid molecule and said target substance, prior to the step of performing the electrophoresis. 
     
     
         5 . The method for obtaining oligonucleotide according to  claim 1 , wherein said oligonucleotide is RNA aptamer. 
     
     
         6 . The method for obtaining oligonucleotide according to  claim 1 , wherein said target substance is protein

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