Wound or tissue dressing comprising lactic acid bacteria

Abstract

The present invention is directed to a wound or tissue dressing comprising a bacteria having the property of producing lactic acid from sugars by fermentation of the sugars. The bacteria preferably belongs to the family of lactic acid bacteria. The family of lactic acid bacteria refers to any bacteria belonging to a genus selected from the group consisting of Carnobacterium, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Streptococcus, Tetragenococcus, Vagococcus and Weissella . There is also provided a wound or tissue dressing comprising an absorbent compound for absorbing wound exudate, wherein said wound or tissue dressing is attached to or comprises a lactic acid bacterium. The utility of the present invention is demonstrated by use of the wound or tissue dressings in methods for treating a wound or damaged tissue in an individual, said method comprising the steps of contacting said wound or damaged tissue with the wound or tissue dressing according to the invention, thereby treating the wound or damaged tissue. The treatment results in healing of the wound or in accelerated healing of the wound. There is also provided the use of a lactic acid bacteria in the manufacture of a wound or tissue dressing for treating or accelerating the healing of a wound in an individual.

1 . A wound or tissue dressing comprising lactic acid producing bacteria, said dressing comprising an absorbent compound for absorbing wound exudate, wherein the absorbent compound and the lactic acid producing bacteria are present in different compartments of the dressing, wherein the lactic acid producing bacteria, or their metabolites, can migrate from the compartment containing the lactic acid producing bacteria to the wound environment. 
     
     
         2 - 20 . (canceled) 
     
     
         21 . The wound or tissue dressing according to  claim 1 , wherein the absorbent compound comprises or consists of gelatin and/or collagen. 
     
     
         22 - 45 . (canceled) 
     
     
         46 . The wound or tissue dressing according to  claim 1 , wherein the wound or tissue dressing further comprises hyaluronic acid, or a derivative thereof. 
     
     
         47 . The wound or tissue dressing according to  claim 1 , wherein the lactic acid bacteria is selected from the group consisting of  Carnobacterium, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Streptococcus, Tetragenococcus, Vagococcus  and  Weissella.    
     
     
         48 . The wound or tissue dressing according to  claim 1 , wherein the lactic acid producing bacteria is present in said different compartment by physical entrapment. 
     
     
         49 . The wound or tissue dressing according to  claim 48  wherein said physical entrapment is within a matrix present in said different compartment. 
     
     
         50 . The wound or tissue dressing according to  claim 1 , wherein the lactic acid producing bacteria is present in said different compartment by chemical bonding to a matrix. 
     
     
         51 . The wound or tissue dressing according to  claim 50  wherein said chemical bonding to a matrix is a gelatin and/or collagen matrix. 
     
     
         52 - 56 . (canceled) 
     
     
         57 . The wound or tissue dressing according to  claim 1 , wherein the absorbent compound is in contact with one or more compartment(s) comprising the lactic acid producing bacteria. 
     
     
         58 . The wound or tissue dressing according to  claim 57 , wherein the compartment comprising the lactic acid producing bacteria is positioned on the proximal side of the absorbent compound between the absorbent compound and the wound. 
     
     
         59 . (canceled) 
     
     
         60 . The wound or tissue dressing according to  claim 1 , wherein the compartment comprising the lactic acid producing bacteria comprises different membrane portions, wherein each portion has permeable or semi-permeable characteristics with respect to the migration of the lactic acid producing bacteria and their metabolites. 
     
     
         61 . The wound or tissue dressing according to  claim 60 , wherein one area or side of the compartment comprising the lactic acid producing bacteria comprises a membrane portion allowing migration of the lactic acid producing bacteria to the wound, whereas another side or area of the compartment comprising the lactic acid producing bacteria allows migration to the absorbent compound of metabolites only. 
     
     
         62 - 64 . (canceled) 
     
     
         65 . The wound or tissue dressing according to  claim 1 , wherein said dressing protected from undesirable contamination by an outer packaging comprising a sterile barrier seal in the form of an unbroken seal. 
     
     
         66 . A method for manufacturing the wound or tissue dressing according to  claim 1 , said method comprising the steps of providing a lactic acid producing bacteria, attaching said lactic acid producing bacteria with the wound or tissue dressing in a compartment different from the absorbent compound of the wound or tissue dressing, thereby obtaining the wound or tissue dressing according to any of  claim 1 . 
     
     
         67 - 98 . (canceled) 
     
     
         99 . A method for manufacturing a wound or tissue dressing for treating a wound or tissue or accelerating the healing of a wound or tissue in an individual comprising the steps of:
 i) providing a lactic acid bacteria,   ii) providing a wound or tissue dressing,   iii) incorporating said lactic acid bacteria into said wound or tissue dressing,   thereby obtaining a wound or tissue dressing for treating a wound or tissue or accelerating the healing of a wound or tissue in an individual.   
     
     
         100 . A method for manufacturing an absorbent compound of a wound or tissue dressing for treating a wound or tissue or accelerating the healing of a wound or tissue in an individual comprising the steps of:
 i) providing a lactic acid bacteria,   ii) providing an absorbent compound of a wound or tissue dressing,   iii) incorporating said lactic acid bacteria into said absorbent compound,   thereby obtaining an absorbent compound of a wound or tissue dressing for treating a wound or tissue or accelerating the healing of a wound or tissue in an individual.   
     
     
         101 . A method for manufacturing a wound or tissue dressing for preventing or reducing the risk of wound or tissue infection in an individual having suffered a wound comprising the steps of:
 i) providing a lactic acid bacteria,   ii) providing a wound or tissue dressing,   iii) incorporating said lactic acid bacteria into said wound or tissue dressing,   thereby obtaining a wound or tissue dressing for preventing or reducing the risk of wound or tissue infection in an individual having suffered a wound.   
     
     
         102 . A method for manufacturing a wound or tissue dressing for promoting haemostasis in a wound in an individual comprising the steps of:
 i) providing a lactic acid bacteria,   ii) providing a wound or tissue dressing,   iii) incorporating said lactic acid bacteria into said wound or tissue dressing,   thereby obtaining a wound or tissue dressing for promoting haemostasis in a wound in an individual.   
     
     
         103 . A wound or tissue dressing comprising lactic acid producing bacteria, said dressing comprising an absorbent compound for absorbing wound exudate, wherein the absorbent compound and the lactic acid producing bacteria are present in different compartments of the dressing, wherein the lactic acid producing bacteria, or their metabolites, can migrate from the compartment containing the lactic acid producing bacteria to the wound environment, said wound or tissue dressing being for treating a wound or tissue or accelerating the healing of a wound or tissue in an individual, for preventing or reducing the risk of wound or tissue infection in an individual having suffered a wound, or for promoting haemostasis in a wound in an individual.