US2010173288A1PendingUtilityA1
Serum/plasma micronas and uses thereof
Est. expiryNov 2, 2027(~1.3 yrs left)· nominal 20-yr term from priority
C12Q 1/6886C12Q 1/6883C12Q 2600/158C12Q 1/6809C12Q 1/6876C12Q 2600/178
65
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Claims
Abstract
This invention provides a combination of microRNAs for evaluating the physiological and/or pathological condition of a subject, wherein the combination comprises all detectable microRNAs stably existing in the serum/plasma of a subject; and a method for evaluating the physiological and/or pathological condition of a subject, wherein the method includes determining all detectable microRNAs stably existing in the serum/plasma of a subject; and a kit for evaluating the physiological and/or pathological condition of a subject, wherein the kit contains the tools for determining all detectable microRNAs that stably existing in the serum/plasma of a subject; and a biochip for evaluating the physiological and/or pathological condition of a subject, wherein the biochip contains the components for determining all detectable microRNAs stably existing in the serum/plasma of a subject. The aforementioned combination, method, kit and biochip can be used for diagnosis as well as differentially diagnosis of diseases including various tumors; various acute/chronic infectious diseases, e.g. viral diseases such as viral influenza, viral hepatitis, AIDS, SARS, bacterial diseases such as tuberculosis, bacterial pneumonia, and other acute/chronic infectious diseases caused by various pathogenic microorganisms; other acute/chronic diseases such as diseases of respiratory system, diseases of immune system, diseases of blood and hematopoietic system, diseases of circulatory system such as cardio-cerebrovascular diseases, metabolic diseases of endocrine system, diseases of digestive system, diseases of nervous system, diseases of urinary system, diseases of reproductive system and diseases of locomotor system, prediction of complications occurrence and malignant diseases relapse, evaluation of therapeutic effects, screening of pharmaceutical active ingredients, assessment of drug efficacy as well as forensic authentication and prohibited drug inspection and the like, possessing a number of advantages such as extensive detection spectrum, high sensitivity, low cost, convenience for sampling, ease for sample preservation, etc. The said method can be widely used in work related to general survey of diseases and so on, improve the low-specificity and low-sensitivity caused by individual differences which single markers are difficult to overcome, significantly increasing the clinical detection rate of diseases, all of which make it become an effective means for diagnosing diseases in an early phase.
Claims
exact text as granted — not AI-modified1 . A combination of microRNAs for evaluating the physiological and/or pathological condition of a subject, wherein the combination comprises all detectable microRNAs stably existing in the serum/plasma of the subject.
2 . The combination according to claim 1 , characterized in that said all detectable microRNAs stably existing in the serum/plasma of a subject are all mature microRNAs in human serum/plasma.
3 . The combination according to claim 1 , characterized in that said all detectable microRNAs stably existing in the serum/plasma of a subject are let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g, let-7i, miR-1, miR-100, miR-101, miR-103, miR-105, miR-106a, miR-106b, miR-107, miR-10a, miR-10b, miR-122a, miR-124a, miR-125a, miR-125b, miR-126, miR-126*, miR-127, miR-128a, miR-128b, miR-129, miR-130a, miR-130b, miR-132, miR-133a, miR-133b, miR-134, miR-135a, miR-135b, miR-136, miR-137, miR-138, miR-139, miR-140, miR-141, miR-142-3p, miR-142-5p, miR-143, miR-144, miR-145, miR-146a, miR-146b, miR-147, miR-148a, miR-148b, miR-149, miR-150, miR-151, miR-152, miR-153, miR-154, miR-154*, miR-155, miR-15a, miR-15b, miR-16, miR-17-3p, miR-17-5p, miR-181a, miR-181b, miR-181c, miR-181d, miR-182, miR-182*, miR-183, miR-184, miR-185, miR-186, miR-187, miR-188, miR-189, miR-18a, miR-18a*, miR-18b, miR-190, miR-191, miR-191*, miR-192, miR-193a, miR-193b, miR-194, miR-195, miR-196a, miR-196b, miR-197, miR-198, miR-199a, miR-199a*, miR-199b, miR-19a, miR-19b, miR-200a, miR-200a*, miR-200b, miR-200c, miR-202, miR-202*, miR-203, miR-204, miR-205, miR-206, miR-208, miR-20a, miR-20b, miR-21, miR-210, miR-211, miR-212, miR-213, miR-214, miR-215, miR-216, miR-217, miR-218, miR-219, miR-22, miR-220, miR-221, miR-222, miR-223, miR-224, miR-23a, miR-23b, miR-24, miR-25, miR-26a, miR-26b, miR-27a, miR-27b, miR-28, miR-296, miR-299-3p, miR-299-5p, miR-29a, miR-29b, miR-29c, miR-301, miR-302a, miR-302a*, miR-302b, miR-302b*, miR-302c, miR-302c*, miR-302d, miR-30a-3p, miR-30a-5p, miR-30b, miR-30c, miR-30d, miR-30e-3p, miR-30e-5p, miR-31, miR-32, miR-320, miR-323, miR-324-3p, miR-324-5p, miR-325, miR-326, miR-328, miR-329, miR-33, miR-330, miR-331, miR-335, miR-337, miR-338, miR-339, miR-33b, miR-340, miR-342, miR-345, miR-346, miR-34a, miR-34b, miR-34c, miR-361, miR-362, miR-363, miR-363*, miR-365, miR-367, miR-368, miR-369-3p, miR-369-5p, miR-370, miR-371, miR-372, miR-373, miR-373*, miR-374, miR-375, miR-376a, miR-376a*, miR-376b, miR-377, miR-378, miR-379, miR-380-3p, miR-380-5p, miR-381, miR-382, miR-383, miR-384, miR-409-3p, miR-409-5p, miR-410, miR-411, miR-412, miR-421, miR-422a, miR-422b, miR-423, miR-424, miR-425, miR-425-5p, miR-429, miR-431, miR-432, miR-432*, miR-433, miR-448, miR-449, miR-450, miR-451, miR-452, miR-452*, miR-453, miR-455, miR-483, miR-484, miR-485-3p, miR-485-5p, miR-486, miR-487a, miR-487b, miR-488, miR-489, miR-490, miR-491, miR-492, miR-493, miR-493-3p, miR-494, miR-495, miR-496, miR-497, miR-498, miR-499, miR-500, miR-501, miR-502, miR-503, miR-504, miR-505, miR-506, miR-507, miR-508, miR-509, miR-510, miR-511, miR-512-3p, miR-512-5p, miR-513, miR-514, miR-515-3p, miR-515-5p, miR-516-3p, miR-516-5p, miR-517*, miR-517a, miR-517b, miR-517c, miR-518a, miR-518a-2*, miR-518b, miR-518c, miR-518c*, miR-518d, miR-518e, miR-518f, miR-518f*, miR-519a, miR-519b, miR-519c, miR-519d, miR-519e, miR-519e*, miR-520a, miR-520a*, miR-520b, miR-520c, miR-520d, miR-520d*, miR-520e, miR-520f, miR-520g, miR-520h, miR-521, miR-522, miR-523, miR-524, miR-524*, miR-525, miR-525*, miR-526a, miR-526b, miR-526b*, miR-526c, miR-527, miR-532, miR-542-3p, miR-542-5p, miR-544, miR-545, miR-548a, miR-548b, miR-548c, miR-548d, miR-549, miR-550, miR-551a, miR-552, miR-553, miR-554, miR-555, miR-556, miR-557, miR-558, miR-559, miR-560, miR-561, miR-562, miR-563, miR-564, miR-565, miR-566, miR-567, miR-568, miR-569, miR-570, miR-571, miR-572, miR-573, miR-574, miR-575, miR-576, miR-577, miR-578, miR-579, miR-580, miR-581, miR-582, miR-583, miR-584, miR-585, miR-586, miR-587, miR-588, miR-589, miR-590, miR-591, miR-592, miR-593, miR-594, miR-595, miR-596, miR-597, miR-598, miR-599, miR-600, miR-601, miR-602, miR-603, miR-604, miR-605, miR-606, miR-607, miR-608, miR-609, miR-610, miR-611, miR-612, miR-613, miR-615, miR-616, miR-617, miR-618, miR-619, miR-620, miR-621, miR-622, miR-623, miR-624, miR-625, miR-626, miR-627, miR-628, miR-629, miR-630, miR-631, miR-632, miR-633, miR-634, miR-635, miR-636, miR-637, miR-638, miR-639, miR-640, miR-641, miR-642, miR-643, miR-644, miR-645, miR-646, miR-647, miR-648, miR-649, miR-650, miR-651, miR-652, miR-653, miR-654, miR-655, miR-656, miR-657, miR-658, miR-659, miR-660, miR-661, miR-662, miR-663, miR-7, miR-9, miR-9*, miR-92, miR-93, miR-95, miR-96, miR-98, miR-99a and miR-99b.
4 . The combination according to claim 1 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to determine physiological and/or pathological condition of the subject after being administrated a test sample.
5 . The combination according to claim 4 , characterized in that the combination is useful for screening the test sample for the activities on the prevention and/or treatment of diseases.
6 . The combination according claim 1 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to diagnose and/or differentially diagnose the diseases of the subject.
7 . The combination according to claim 1 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to evaluate the effectiveness of treating the diseases of the subject.
8 . The combination of according to claim 1 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to predict the occurrence of diseases of the subject.
9 . The combination according to claim 8 , characterized in that the occurrence of diseases is the occurrence of complications and/or the relapse of malignant diseases.
10 . The combination according to claim 5 , characterized in that the diseases include various tumors; various acute and chronic infectious diseases, e.g. viral diseases such as viral influenza, viral hepatitis, AIDS, SARS, bacterial diseases such as tuberculosis, bacterial pneumonia, and other acute and chronic infectious diseases caused by various pathogenic microorganisms; other acute and chronic diseases such as diseases of respiratory system, diseases of immune system, diseases of blood and hematopoietic system, diseases of circulatory system such as cardio-cerebrovascular diseases, metabolic diseases of endocrine system, diseases of digestive system, diseases of nervous system, diseases of urinary system, diseases of reproductive system and diseases of locomotor system.
11 . The combination according to claim 1 , characterized in that the combination is useful for detecting the subject for prohibited drugs-taking.
12 . The combination according to claim 1 , characterized in that the serum/plasma derives from living body, tissues, organs and/or corpuses of the subject.
13 . A method for evaluating the physiological and/or pathological condition of a subject, wherein the method comprises determining all detectable microRNAs stably existing in serum/plasma of the subject.
14 . The method according to claim 13 , characterized in that said all detectable microRNAs stably existing in serum/plasma of a subject are all mature microRNAs in human serum/plasma.
15 . The method according to claim 13 , characterized in that said all detectable microRNAs stably existing in serum/plasma of a subjects are let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g, let-7i, miR-1, miR-100, miR-101, miR-103, miR-105, miR-106a, miR-106b, miR-107, miR-10a, miR-10b, miR-122a, miR-124a, miR-125a, miR-125b, miR-126, miR-126*, miR-127, miR-128a, miR-128b, miR-129, miR-130a, miR-130b, miR-132, miR-133a, miR-133b, miR-134, miR-135a, miR-135b, miR-136, miR-137, miR-138, miR-139, miR-140, miR-141, miR-142-3p, miR-142-5p, miR-143, miR-144, miR-145, miR-146a, miR-146b, miR-147, miR-148a, miR-148b, miR-149, miR-150, miR-151, miR-152, miR-153, miR-154, miR-154*, miR-155, miR-15a, miR-15b, miR-16, miR-17-3p, miR-17-5p, miR-181a, miR-181b, miR-181c, miR-181d, miR-182, miR-182*, miR-183, miR-184, miR-185, miR-186, miR-187, miR-188, miR-189, miR-18a, miR-18a*, miR-18b, miR-190, miR-191, miR-191*, miR-192, miR-193a, miR-193b, miR-194, miR-195, miR-196a, miR-196b, miR-197, miR-198, miR-199a, miR-199a*, miR-199b, miR-19a, miR-19b, miR-200a, miR-200a*, miR-200b, miR-200c, miR-202, miR-202*, miR-203, miR-204, miR-205, miR-206, miR-208, miR-20a, miR-20b, miR-21, miR-210, miR-211, miR-212, miR-213, miR-214, miR-215, miR-216, miR-217, miR-218, miR-219, miR-22, miR-220, miR-221, miR-222, miR-223, miR-224, miR-23a, miR-23b, miR-24, miR-25, miR-26a, miR-26b, miR-27a, miR-27b, miR-28, miR-296, miR-299-3p, miR-299-5p, miR-29a, miR-29b, miR-29c, miR-301, miR-302a, miR-302a*, miR-302b, miR-302b*, miR-302c, miR-302c*, miR-302d, miR-30a-3p, miR-30a-5p, miR-30b, miR-30c, miR-30d, miR-30e-3p, miR-30e-5p, miR-31, miR-32, miR-320, miR-323, miR-324-3p, miR-324-5p, miR-325, miR-326, miR-328, miR-329, miR-33, miR-330, miR-331, miR-335, miR-337, miR-338, miR-339, miR-33b, miR-340, miR-342, miR-345, miR-346, miR-34a, miR-34b, miR-34c, miR-361, miR-362, miR-363, miR-363*, miR-365, miR-367, miR-368, miR-369-3p, miR-369-5p, miR-370, miR-371, miR-372, miR-373, miR-373*, miR-374, miR-375, miR-376a, miR-376a*, miR-376b, miR-377, miR-378, miR-379, miR-380-3p, miR-380-5p, miR-381, miR-382, miR-383, miR-384, miR-409-3p, miR-409-5p, miR-410, miR-411, miR-412, miR-421, miR-422a, miR-422b, miR-423, miR-424, miR-425, miR-425-5p, miR-429, miR-431, miR-432, miR-432*, miR-433, miR-448, miR-449, miR-450, miR-451, miR-452, miR-452*, miR-453, miR-455, miR-483, miR-484, miR-485-3p, miR-485-5p, miR-486, miR-487a, miR-487b, miR-488, miR-489, miR-490, miR-491, miR-492, miR-493, miR-493-3p, miR-494, miR-495, miR-496, miR-497, miR-498, miR-499, miR-500, miR-501, miR-502, miR-503, miR-504, miR-505, miR-506, miR-507, miR-508, miR-509, miR-510, miR-511, miR-512-3p, miR-512-5p, miR-513, miR-514, miR-515-3p, miR-515-5p, miR-516-3p, miR-516-5p, miR-517*, miR-517a, miR-517b, miR-517c, miR-518a, miR-518a-2*, miR-518b, miR-518c, miR-518c*, miR-518d, miR-518e, miR-518f, miR-518f*, miR-519a, miR-519b, miR-519c, miR-519d, miR-519e, miR-519e*, miR-520a, miR-520a*, miR-520b, miR-520c, miR-520d, miR-520d*, miR-520e, miR-520f, miR-520g, miR-520h, miR-521, miR-522, miR-523, miR-524, miR-524*, miR-525, miR-525*, miR-526a, miR-526b, miR-526b*, miR-526c, miR-527, miR-532, miR-542-3p, miR-542-5p, miR-544, miR-545, miR-548a, miR-548b, miR-548c, miR-548d, miR-549, miR-550, miR-551a, miR-552, miR-553, miR-554, miR-555, miR-556, miR-557, miR-558, miR-559, miR-560, miR-561, miR-562, miR-563, miR-564, miR-565, miR-566, miR-567, miR-568, miR-569, miR-570, miR-571, miR-572, miR-573, miR-574, miR-575, miR-576, miR-577, miR-578, miR-579, miR-580, miR-581, miR-582, miR-583, miR-584, miR-585, miR-586, miR-587, miR-588, miR-589, miR-590, miR-591, miR-592, miR-593, miR-594, miR-595, miR-596, miR-597, miR-598, miR-599, miR-600, miR-601, miR-602, miR-603, miR-604, miR-605, miR-606, miR-607, miR-608, miR-609, miR-610, miR-611, miR-612, miR-613, miR-614, miR-615, miR-616, miR-617, miR-618, miR-619, ma-620, miR-621, miR-622, miR-623, miR-624, miR-625, miR-626, miR-627, miR-628, miR-629, miR-630, miR-631, miR-632, miR-633, miR-634, miR-635, miR-636, miR-637, miR-638, miR-639, miR-640, miR-641, miR-642, miR-643, miR-644, miR-645, miR-646, miR-647, miR-648, miR-649, miR-650, miR-651, miR-652, miR-653, miR-654, miR-655, miR-656, miR-657, miR-658, miR-659, miR-660, miR-661, miR-662, miR-663, miR-7, miR-9, miR-9*, miR-92, miR-93, miR-95, miR-96, miR-98, miR-99a and miR-99b.
16 . The method according to claim 13 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to determine the physiological and/or pathological condition of the subject after being administrated a test sample.
17 . The method according to claim 16 , characterized in that the method is useful for screening the test sample for the activities on the prevention and/or treatment of diseases.
18 . The method according to claim 13 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to diagnose and/or differentially diagnose the diseases of the subject.
19 . The method according to claim 13 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to evaluate the effectiveness of treating the diseases of the subject.
20 . The method according to claim 13 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to predict the occurrence of diseases of the subject.
21 . The method according to claim 20 , characterized in that the occurrence of diseases is the occurrence of complications and/or the relapse of malignant diseases.
22 . The method according to claim 17 , characterized in that the diseases include various tumors; various acute and chronic infectious diseases e.g. viral diseases such as viral influenza, viral hepatitis, AIDS, SARS; bacterial diseases such as tuberculosis, bacterial pneumonia, and other acute and chronic infectious diseases caused by various pathogenic microorganisms; other acute and chronic diseases such as diseases of respiratory system, diseases of immune system, diseases of blood and hematopoietic system, diseases of circulatory system such as cardio-cerebrovascular disease, metabolic diseases of endocrine system, diseases of digestive system, diseases of nervous system, diseases of urinary system, diseases of reproductive system and diseases of locomotor system.
23 . The method according to claim 13 , characterized in that the method is useful for detecting the subject for prohibited drugs-taking.
24 . The method according to claim 13 , characterized in that the method for determining all detectable microRNAs stably existing in serum/plasma of a subject is one or more selected from the group consisting of RT-PCR method, Real-time PCR method, Northern blotting method, RNase protection assay, Solexa sequencing technology and biochip method.
25 . The method according to claim 24 , characterized in that the method is RT-PCR method which includes the following steps:
(1) extracting the total RNA from the serum/plasma of the subject, and obtaining cDNA samples by RNA reverse transcription reaction; or collecting serum/plasma samples from the subject and conducting reverse transcription reaction with serum/plasma being a buffer so as to prepare cDNA samples; (2) designing a primer by use of microRNAs and conducting PCR reaction; (3) conducting agarose gel electrophoresis of PCR products; and (4) observing agarose gel under ultraviolet Lamp after EB staining.
26 . The method according to claim 24 , characterized in that the method is Real-time PCR which includes the following steps:
(1) extracting the total RNA from the serum/plasma of the subject, and obtaining cDNA samples by RNA reverse transcription reaction; or collecting serum/plasma samples from the subject, preparing cDNA samples by reverse transcription reaction with serum/plasma being a buffer; (2) designing a primer by use of microRNAs; (3) adding a fluorescent probe to conduct PCR reaction; (4) detecting and comparing the variation in levels of microRNAs in the serum/plasma samples relative to those of microRNAs in normal serum/plasma.
27 . The method according to claim 13 , characterized in that the serum/plasma derives from living bodies, tissues, organs and/or corpuses of the subject.
28 . A kit for evaluating the physiological and/or pathological condition of a subject, wherein the kit comprises the tools useful for determining all detectable microRNAs stably existing in the serum/plasma of the subject.
29 . The kit according to claim 28 , characterized in that the kit comprises the primers of all mature microRNAs in human serum/plasma.
30 . The kit according to claim 28 , characterized in that the kit comprises the primers of let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g, let-7i, miR-1, miR-100, miR-101, miR-103, miR-105, miR-106a, miR-106b, miR-107, miR-10a, miR-10b, miR-122a, miR-124a, miR-125a, miR-125b, miR-126, miR-126*, miR-127, miR-128a, miR-128b, miR-129, miR-130a, miR-130b, miR-132, miR-133a, miR-133b, miR-134, miR-135a, miR-135b, miR-136, miR-137, miR-138, miR-139, miR-140, miR-141, miR-142-3p, miR-142-5p, miR-143, miR-144, miR-145, miR-146a, miR-146b, miR-147, miR-148a, miR-148b, miR-149, miR-150, miR-151, miR-152, miR-153, miR-154, miR-154*, miR-155, miR-15a, miR-15b, miR-16, miR-17-3p, miR-17-5p, miR-181a, miR-181b, miR-181c, miR-181d, miR-182, miR-182*, miR-183, miR-184, miR-185, miR-186, miR-187, miR-188, miR-189, miR-18a, miR-18a*, miR-18b, miR-190, miR-191, miR-191*, miR-192, miR-193a, miR-193b, miR-194, miR-195, miR-196a, miR-196b, miR-197, miR-198, miR-199a, miR-199a*, miR-199b, miR-19a, miR-19b, miR-200a, miR-200a*, miR-200b, miR-200c, miR-202, miR-202*, miR-203, miR-204, miR-205, miR-206, miR-208, miR-20a, miR-20b, miR-21, miR-210, miR-211, miR-212, miR-213, miR-214, miR-215, miR-216, miR-217, miR-218, miR-219, miR-22, miR-220, miR-221, miR-222, miR-223, miR-224, miR-23a, miR-23b, miR-24, miR-25, miR-26a, miR-26b, miR-27a, miR-27b, miR-28, miR-296, miR-299-3p, miR-299-5p, miR-29a, miR-29b, miR-29c, miR-301, miR-302a, miR-302a*, miR-302b, miR-302b*, miR-302c, miR-302c*, miR-302d, miR-30a-3p, miR-30a-5p, miR-30b, miR-30c, miR-30d, miR-30e-3p, miR-30e-5p, miR-31, miR-32, miR-320, miR-323, miR-324-3p, miR-324-5p, miR-325, miR-326, miR-328, miR-329, miR-33, miR-330, miR-331, miR-335, miR-337, miR-338, miR-339, miR-33b, miR-340, miR-342, miR-345, miR-346, miR-34a, miR-34b, miR-34c, miR-361, miR-362, miR-363, miR-363*, miR-365, miR-367, miR-368, miR-369-3p, miR-369-5p, miR-370, miR-371, miR-372, miR-373, miR-373*, miR-374, miR-375, miR-376a, miR-376a*, miR-376b, miR-377, miR-378, miR-379, miR-380-3p, miR-380-5p, miR-381, miR-382, miR-383, miR-384, miR-409-3p, miR-409-5p, miR-410, miR-411, miR-412, miR-421, miR-422a, miR-422b, miR-423, miR-424, miR-425, miR-425-5p, miR-429, miR-431, miR-432, miR-432*, miR-433, miR-448, miR-449, miR-450, miR-451, miR-452, miR-452*, miR-453, miR-455, miR-483, miR-484, miR-485-3p, miR-485-5p, miR-486, miR-487a, miR-487b, miR-488, miR-489, miR-490, miR-491, miR-492, miR-493, miR-493-3p, miR-494, miR-495, miR-496, miR-497, miR-498, miR-499, miR-500, miR-501, miR-502, miR-503, miR-504, miR-505, miR-506, miR-507, miR-508, miR-509, miR-510, miR-511, miR-512-3p, miR-512-5p, miR-513, miR-514, miR-515-3p, miR-515-5p, miR-516-3p, miR-516-5p, miR-517*, miR-517a, miR-517b, miR-517c, miR-518a, miR-518a-2*, miR-518b, miR-518c, miR-518c*, miR-518d, miR-518e, miR-518f, miR-518f*, miR-519a, miR-519b, miR-519c, miR-519d, miR-519e, miR-519e*, miR-520a, miR-520a*, miR-520b, miR-520c, miR-520d, miR-520d*, miR-520e, miR-520f, miR-520g, miR-520h, miR-521, miR-522, miR-523, miR-524, miR-524*, miR-525, miR-525*, miR-526a, miR-526b, miR-526b*, miR-526c, miR-527, miR-532, miR-542-3p, miR-542-5p, miR-544, miR-545, miR-548a, miR-548b, miR-548c, miR-548d, miR-549, miR-550, miR-551a, miR-552, miR-553, miR-554, miR-555, miR-556, miR-557, miR-558, miR-559, miR-560, miR-561, miR-562, miR-563, miR-564, miR-565, miR-566, miR-567, miR-568, miR-569, miR-570, miR-571, miR-572, miR-573, miR-574, miR-575, miR-576, miR-577, miR-578, miR-579, miR-580, miR-581, miR-582, miR-583, miR-584, miR-585, miR-586, miR-587, miR-588, miR-589, miR-590, miR-591, miR-592, miR-593, miR-594, miR-595, miR-596, miR-597, miR-598, miR-599, miR-600, miR-601, miR-602, miR-603, miR-604, miR-605, miR-606, miR-607, miR-608, miR-609, miR-610, miR-611, miR-612, miR-613, miR-614, miR-615, miR-616, miR-617, miR-618, miR-619, miR-620, miR-621, miR-622, miR-623, miR-624, miR-625, miR-626, miR-627, miR-628, miR-629, miR-630, miR-631, miR-632, miR-633, miR-634, miR-635, miR-636, miR-637, miR-638, miR-639, miR-640, miR-641, miR-642, miR-643, miR-644, miR-645, miR-646, miR-647, miR-648, miR-649, miR-650, miR-651, miR-652, miR-653, miR-654, miR-655, miR-656, miR-657, miR-658, miR-659, miR-660, miR-661, miR-662, miR-663, miR-7, miR-9, miR-9*, miR-92, miR-93, miR-95, miR-96, miR-98, miR-99a and miR-99b.
31 . The kit according to claim 28 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to determine the physiological and/or pathological condition of a subject after being administrated a test sample.
32 . The kit according to claim 31 , characterized in that the kit is useful for screening the test sample for the activities on the prevention and/or treatment of diseases.
33 . The kit according to claim 28 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to diagnose and/or differentially diagnose the diseases of the subject.
34 . The kit according to claim 28 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to evaluate the effectiveness of treating the diseases of the subject.
35 . The kit according to claim 28 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to predict the occurrence of diseases of the subject.
36 . The kit according to claim 35 , characterized in that the occurrence of diseases is the occurrence of complications and/or the relapse of malignant diseases.
37 . The kit according to claim 32 , characterized in that the diseases include various tumors, various acute and chronic infectious diseases e.g. viral diseases such as viral influenza, viral hepatitis, AIDS, SARS, bacterial diseases such as tuberculosis, bacterial pneumonia, and other acute and chronic infectious diseases caused by various pathogenic microorganisms; other acute and chronic diseases such as diseases of respiratory system, diseases of immune system, diseases of blood and hematopoietic system, diseases of circulatory system such as cardio-cerebrovascular disease, metabolic diseases of endocrine system, diseases of digestive system, diseases of nervous system, diseases of urinary system, diseases of reproductive system and diseases of locomotor system.
38 . The kit according to claim 28 , characterized in that the kit is useful for detecting the subject for prohibited drugs-taking.
39 . The kit according to claim 28 , characterized in that the serum/plasma of the subject are from living bodies, tissues, organs and/or corpuses of the subject.
40 . A biochip for evaluating the physiological and/or pathological condition of a subject, wherein the biochip contains components useful for determining all detectable microRNAs stably existing in the serum/plasma of the subject.
41 . The biochip according to claim 40 , characterized in that the biochip contains the probes for all mature microRNAs in human serum/plasma.
42 . The biochip according to claim 40 , characterized in that the biochip comprises the following probes:
Corre-
sponding
micro-
Probes
RNas
Sequences of probes
probe-let-7a
let-7a
AACTATACAACCTACTACCTCA
probe-let-7b
let-7b
AACCACACAACCTACTACCTCA
probe-let-7c
let-7c
AACCATACAACCTACTACCTCA
probe-let-7d
let-7d
ACTATGCAACCTACTACCTCT
probe-let-7e
let-7e
ACTATACAACCTCCTACCTCA
probe-let-7f
let-7f
AACTATACAATCTACTACCTCA
probe-let-7g
let-7g
ACTGTACAAACTACTACCTCA
probe-let-7i
let-7i
ACAGCACAAACTACTACCTCA
probe-miR-1
miR-1
TACATACTTCTTTACATTCCA
probe-miR-100
miR-100
CACAAGTTCGGATCTACGGGTT
probe-miR-101
miR-101
CTTCAGTTATCACAGTACTGTA
probe-miR-103
miR-103
TCATAGCCCTGTACAATGCTGCT
probe-miR-105
miR-105
ACAGGAGTCTGAGCATTTGA
probe-miR-106a
miR-106a
GCTACCTGCACTGTAAGCACTTTT
probe-miR-106b
miR-106b
ATCTGCACTGTCAGCACTTTA
probe-miR-107
miR-107
TGATAGCCCTGTACAATGCTGCT
probe-miR-10a
miR-10a
CACAAATTCGGATCTACAGGGTA
probe-miR-10b
miR-10b
ACAAATTCGGTTCTACAGGGTA
probe-miR-122a
miR-122a
ACAAACACCATTGTCACACTCCA
probe-miR-124a
miR-124a
TGGCATTCACCGCGTGCCTTAA
probe-miR-125a
miR-125a
CACAGGTTAAAGGGTCTCAGGGA
probe-miR-125b
miR-125b
TCACAAGTTAGGGTCTCAGGGA
probe-miR-126
miR-126
GCATTATTACTCACGGTACGA
probe-miR-126*
miR-126*
CGCGTACCAAAAGTAATAATG
probe-miR-127
miR-127
AGCCAAGCTCAGACGGATCCGA
probe-miR-128a
miR-128a
AAAAGAGACCGGTTCACTGTGA
probe-miR-128b
miR-128b
GAAAGAGACCGGTTCACTGTGA
probe-miR-129
miR-129
GCAAGCCCAGACCGCAAAAAG
probe-miR-130a
miR-130a
ATGCCCTTTTAACATTGCACTG
probe-miR-130b
miR-130b
ATGCCCTTTCATCATTGCACTG
probe-miR-132
miR-132
CGACCATGGCTGTAGACTGTTA
probe-miR-133a
miR-133a
ACAGCTGGTTGAAGGGGACCAA
probe-miR-133b
miR-133b
TAGCTGGTTGAAGGGGACCAA
probe-miR-134
miR-134
CCCTCTGGTCAACCAGTCACA
probe-miR-135a
miR-135a
TCACATAGGAATAAAAAGCCATA
probe-miR-135b
miR-135b
CACATAGGAATGAAAAGCCATA
probe-miR-136
miR-136
TCCATCATCAAAACAAATGGAGT
probe-miR-137
miR-137
CTACGCGTATTCTTAAGCAATA
probe-miR-138
miR-138
GATTCACAACACCAGCT
probe-miR-139
miR-139
AGACACGTGCACTGTAGA
probe-miR-140
miR-140
CTACCATAGGGTAAAACCACT
probe-miR-141
miR-141
CCATCTTTACCAGACAGTGTTA
probe-miR-142-
miR-142-
TCCATAAAGTAGGAAACACTACA
3p
3p
probe-miR-142-
miR-142-
GTAGTGCTTTCTACTTTATG
5p
5p
probe-miR-143
miR-143
TGAGCTACAGTGCTTCATCTCA
probe-miR-144
miR-144
CTAGTACATCATCTATACTGTA
probe-miR-145
miR-145
AAGGGATTCCTGGGAAAACTGGAC
probe-miR-146a
miR-146a
AACCCATGGAATTCAGTTCTCA
probe-miR-146b
miR-146b
AGCCTATGGAATTCAGTTCTCA
probe-miR-147
miR-147
GCAGAAGCATTTCCACACAC
probe-miR-148a
miR-148a
ACAAAGTTCTGTAGTGCACTGA
probe-miR-148b
miR-148b
ACAAAGTTCTGTGATGCACTGA
probe-miR-149
miR-149
GGAGTGAAGACACGGAGCCAGA
probe-miR-150
miR-150
CACTGGTACAAGGGTTGGGAGA
probe-miR-151
miR-151
CCTCAAGGAGCTTCAGTCTAGT
probe-miR-152
miR-152
CCCAAGTTCTGTCATGCACTGA
probe-miR-153
miR-153
TCACTTTTGTGACTATGCAA
probe-miR-154
miR-154
CGAAGGCAACACGGATAACCTA
probe-miR-154*
miR-154*
AATAGGTCAACCGTGTATGATT
probe-miR-155
miR-155
CCCCTATCACGATTAGCATTAA
probe-miR-15a
miR-15a
CACAAACCATTATGTGCTGCTA
probe-miR-15b
miR-15b
TGTAAACCATGATGTGCTGCTA
probe-miR-16
miR-16
CGCCAATATTTACGTGCTGCTA
probe-miR-17-
miR-17-
ACAAGTGCCTTCACTGCAGT
3p
3p
probe-miR-17-
miR-17-
ACTACCTGCACTGTAAGCACTTTG
5p
5p
probe-miR-181a
miR-181a
ACTCACCGACAGCGTTGAATGTT
probe-miR-181b
miR-181b
CCCACCGACAGCAATGAATGTT
probe-miR-181c
miR-181c
ACTCACCGACAGGTTGAATGTT
probe-miR-181d
miR-181d
AACCCACCGACAACAATGAATGTT
probe-miR-182
miR-182
TGTGAGTTCTACCATTGCCAAA
probe-miR-182*
miR-182*
TAGTTGGCAAGTCTAGAACCA
probe-miR-183
miR-183
CAGTGAATTCTACCAGTGCCATA
probe-miR-184
miR-184
ACCCTTATCAGTTCTCCGTCCA
probe-miR-185
miR-185
GAACTGCCTTTCTCTCCA
probe-miR-186
miR-186
AAGCCCAAAAGGAGAATTCTTTG
probe-miR-187
miR-187
CGGCTGCAACACAAGACACGA
probe-miR-188
miR-188
ACCCTCCACCATGCAAGGGATG
probe-miR-189
miR-189
ACTGATATCAGCTCAGTAGGCAC
probe-miR-18a
miR-18a
TATCTGCACTAGATGCACCTTA
probe-miR-18a*
miR-18a*
AGAAGGAGCACTTAGGGCAGT
probe-miR-18b
miR-18b
TAACTGCACTAGATGCACCTTA
probe-miR-190
miR-190
ACCTAATATATCAAACATATCA
probe-miR-191
miR-191
AGCTGCTTTTGGGATTCCGTTG
probe-miR-191*
miR-191*
GGGGACGAAATCCAAGCGCAGC
probe-miR-192
miR-192
GGCTGTCAATTCATAGGTCAG
probe-miR-193a
miR-193a
CTGGGACTTTGTAGGCCAGTT
probe-miR-193b
miR-193b
AAAGCGGGACTTTGAGGGCCAGTT
probe-miR-194
miR-194
TCCACATGGAGTTGCTGTTACA
probe-miR-195
miR-195
GCCAATATTTCTGTGCTGCTA
probe-miR-196a
miR-196a
CCAACAACATGAAACTACCTA
probe-miR-196b
miR-196b
CCAACAACAGGAAACTACCTA
probe-miR-197
miR-197
GCTGGGTGGAGAAGGTGGTGAA
probe-miR-198
miR-198
CCTATCTCCCCTCTGGACC
probe-miR-199a
miR-199a
GAACAGGTAGTCTGAACACTGGG
probe-miR-
miR-199a*
AACCAATGTGCAGACTACTGTA
199a*
probe-miR-199b
miR-199b
GAACAGATAGTCTAAACACTGGG
probe-miR-19a
miR-19a
TCAGTTTTGCATAGATTTGCACA
probe-miR-19b
miR-19b
TCAGTTTTGCATGGATTTGCACA
probe-miR-200a
miR-200a
ACATCGTTACCAGACAGTGTTA
probe-miR-
miR-200a*
TCCAGCACTGTCCGGTAAGATG
200a*
probe-miR-200b
miR-200b
GTCATCATTACCAGGCAGTATTA
probe-miR-200c
miR-200c
CCATCATTACCCGGCAGTATTA
probe-miR-202
miR-202
TTTTCCCATGCCCTATACCTCT
probe-miR-202*
miR-202*
AAAGAAGTATATGCATAGGAAA
probe-miR-203
miR-203
CTAGTGGTCCTAAACATTTCAC
probe-miR-204
miR-204
AGGCATAGGATGACAAAGGGAA
probe-miR-205
miR-205
CAGACTCCGGTGGAATGAAGGA
probe-miR-206
miR-206
CCACACACTTCCTTACATTCCA
probe-miR-208
miR-208
ACAAGCTTTTTGCTCGTCTTAT
probe-miR-20a
miR-20a
CTACCTGCACTATAAGCACTTTA
probe-miR-20b
miR-20b
CTACCTGCACTATGAGCACTTTG
probe-miR-21
miR-21
TCAACATCAGTCTGATAAGCTA
probe-miR-210
miR-210
TCAGCCGCTGTCACACGCACAG
probe-miR-211
miR-211
AGGCGAAGGATGACAAAGGGAA
probe-miR-212
miR-212
GGCCGTGACTGGAGACTGTTA
probe-miR-213
miR-213
GGTACAATCAACGGTCGATGGT
probe-miR-214
miR-214
CTGCCTGTCTGTGCCTGCTGT
probe-miR-215
miR-215
GTCTGTCAATTCATAGGTCAT
probe-miR-216
miR-216
CACAGTTGCCAGCTGAGATTA
probe-miR-217
miR-217
ATCCAATCAGTTCCTGATGCAGTA
probe-miR-218
miR-218
ACATGGTTAGATCAAGCACAA
probe-miR-219
miR-219
AGAATTGCGTTTGGACAATCA
probe-miR-22
miR-22
ACAGTTCTTCAACTGGCAGCTT
probe-miR-220
miR-220
AAAGTGTCAGATACGGTGTGG
probe-miR-221
miR-221
GAAACCCAGCAGACAATGTAGCT
probe-miR-222
miR-222
GAGACCCAGTAGCCAGATGTAGCT
probe-miR-223
miR-223
GGGGTATTTGACAAACTGACA
probe-miR-224
miR-224
TAAACGGAACCACTAGTGACTTG
probe-miR-23a
miR-23a
GGAAATCCCTGGCAATGTGAT
probe-miR-23b
miR-23b
GGTAATCCCTGGCAATGTGAT
probe-miR-24
miR-24
CTGTTCCTGCTGAACTGAGCCA
probe-miR-25
miR-25
TCAGACCGAGACAAGTGCAATG
probe-miR-26a
miR-26a
GCCTATCCTGGATTACTTGAA
probe-miR-26b
miR-26b
AACCTATCCTGAATTACTTGAA
probe-miR-27a
miR-27a
GCGGAACTTAGCCACTGTGAA
probe-miR-27b
miR-27b
GCAGAACTTAGCCACTGTGAA
probe-miR-28
miR-28
CTCAATAGACTGTGAGCTCCTT
probe-miR-296
miR-296
ACAGGATTGAGGGGGGGCCCT
probe-miR-299-
miR-299-
AAGCGGTTTACCATCCCACATA
3p
3p
probe-miR-299-
miR-299-
ATGTATGTGGGACGGTAAACCA
5p
5p
probe-miR-29a
miR-29a
AACCGATTTCAGATGGTGCTA
probe-miR-29b
miR-29b
AACACTGATTTCAAATGGTGCTA
probe-miR-29c
miR-29c
ACCGATTTCAAATGGTGCTA
probe-miR-301
miR-301
GCTTTGACAATACTATTGCACTG
probe-miR-302a
miR-302a
TCACCAAAACATGGAAGCACTTA
probe-miR-
miR-302a*
AAAGCAAGTACATCCACGTTTA
302a
probe-miR-302b
miR-302b
CTACTAAAACATGGAAGCACTTA
probe-miR-
miR-302b*
AGAAAGCACTTCCATGTTAAAGT
302b*
probe-miR-302c
miR-302c
CCACTGAAACATGGAAGCACTTA
probe-miR-
miR-302c*
CAGCAGGTACCCCCATGTTAAA
302c*
probe-miR-302d
miR-302d
ACACTCAAACATGGAAGCACTTA
probe-miR-30a-
miR-30a-
GCTGCAAACATCCGACTGAAAG
3p
3p
probe-miR-30a-
miR-30a-
CTTCCAGTCGAGGATGTTTACA
5p
5p
probe-miR-30b
miR-30b
AGCTGAGTGTAGGATGTTTACA
probe-miR-30c
miR-30c
GCTGAGAGTGTAGGATGTTTACA
probe-miR-30d
miR-30d
CTTCCAGTCGGGGATGTTTACA
probe-miR-30e-
miR-30e-
GCTGTAAACATCCGACTGAAAG
3p
3p
probe-miR-30e-
miR-30e-
TCCAGTCAAGGATGTTTACA
5p
5p
probe-miR-31
miR-31
CAGCTATGCCAGCATCTTGCC
probe-miR-32
miR-32
GCAACTTAGTAATGTGCAATA
probe-miR-320
miR-320
TTCGCCCTCTCAACCCAGCTTTT
probe-miR-323
miR-323
AGAGGTCGACCGTGTAATGTGC
probe-miR-324-3p
miR-324-
CCAGCAGCACCTGGGGCAGTGG
3p
3p
probe-miR-324-
miR-324-
ACACCAATGCCCTAGGGGATGCG
5p
5p
probe-miR-325
miR-325
ACACTTACTGGACACCTACTAGG
probe-miR-326
miR-326
CTGGAGGAAGGGCCCAGAGG
probe-miR-328
miR-328
ACGGAAGGGCAGAGAGGGCCAG
probe-miR-329
miR-329
AAAGAGGTTAACCAGGTGTGTT
probe-miR-33
miR-33
CAATGCAACTACAATGCAC
probe-miR-330
miR-330
TCTCTGCAGGCCGTGTGCTTTGC
probe-miR-331
miR-331
TTCTAGGATAGGCCCAGGGGC
probe-miR-335
miR-335
ACATTTTTCGTTATTGCTCTTGA
probe-miR-337
miR-337
AAAGGCATCATATAGGAGCTGGA
probe-miR-338
miR-338
TCAACAAAATCACTGATGCTGGA
probe-miR-339
miR-339
TGAGCTCCTGGAGGACAGGGA
probe-miR-33b
miR-33b
TGCAATGCAACAGCAATGCAC
probe-miR-340
miR-340
GGCTATAAAGTAACTGAGACGGA
probe-miR-342
miR-342
GACGGGTGCGATTTCTGTGTGAGA
probe-miR-345
miR-345
GCCCTGGACTAGGAGTCAGCA
probe-miR-346
miR-346
AGAGGCAGGCATGCGGGCAGACA
probe-miR-34a
miR-34a
AACAACCAGCTAAGACACTGCCA
probe-miR-34b
miR-34b
CAATCAGCTAATGACACTGCCTA
probe-miR-34c
miR-34c
GCAATCAGCTAACTACACTGCCT
probe-miR-361
miR-361
GTACCCCTGGAGATTCTGATAA
probe-miR-362
miR-362
CTCACACCTAGGTTCCAAGGATT
probe-miR-363
miR-363
TTACAGATGGATACCGTGCAAT
probe-miR-363*
miR-363*
AAATTGCATCGTGATCCACCCG
probe-miR-365
miR-365
ATAAGGATTTTTAGGGGCATTA
probe-miR-367
miR-367
TCACCATTGCTAAAGTGCAATT
probe-miR-368
miR-368
AAACGTGGAATTTCCTCTATGT
probe-miR-369-
miR-369-
AAAGATCAACCATGTATTATT
3p
3p
probe-miR-369-
miR-369-
GCGAATATAACACGGTCGATCT
5p
5p
probe-miR-370
miR-370
CCAGGTTCCACCCCAGCAGGC
probe-miR-371
miR-371
ACACTCAAAAGATGGCGGCAC
probe-miR-372
miR-372
ACGCTCAAATGTCGCAGCACTTT
probe-miR-373
miR-373
ACACCCCAAAATCGAAGCACTTC
probe-miR-373*
miR-373*
GGAAAGCGCCCCCATTTTGAGT
probe-miR-374
miR-374
CACTTATCAGGTTGTATTATAA
probe-miR-375
miR-375
TCACGCGAGCCGAACGAACAAA
probe-miR-376a
miR-376a
ACGTGGATTTTCCTCTATGAT
probe-miR-
miR-376a*
CTCATAGAAGGAGAATCTACC
376a*
probe-miR-376b
miR-376b
AACATGGATTTTCCTCTATGAT
probe-miR-377
miR-377
ACAAAAGTTGCCTTTGTGTGAT
probe-miR-378
miR-378
ACACAGGACCTGGAGTCAGGAG
probe-miR-379
miR-379
TACGTTCCATAGTCTACCA
probe-miR-380-
miR-380-
AAGATGTGGACCATATTACATA
3p
3p
probe-miR-380-
miR-380-
GCGCATGTTCTATGGTCAACCA
5p
5p
probe-miR-381
miR-381
ACAGAGAGCTTGCCCTTGTATA
probe-miR-382
miR-382
CGAATCCACCACGAACAACTTC
probe-miR-383
miR-383
AGCCACAATCACCTTCTGATCT
probe-miR-384
miR-384
TATGAACAATTTCTAGGAAT
probe-miR-409-
miR-409-
AGGGGTTCACCGAGCAACATTCG
3p
3p
probe-miR-409-
miR-409-
TGCAAAGTTGCTCGGGTAACCT
5p
5p
probe-miR-410
miR-410
AACAGGCCATCTGTGTTATATT
probe-miR-411
miR-411
CGTACGCTATACGGTCTACTA
probe-miR-412
miR-412
ACGGCTAGTGGACCAGGTGAAGT
probe-miR-421
miR-421
GCGCCCAATTAATGTCTGTTGAT
probe-miR-422a
miR-422a
GGCCTTCTGACCCTAAGTCCAG
probe-miR-422b
miR-422b
GGCCTTCTGACTCCAAGTCCAG
probe-miR-423
miR-423
CTGAGGGGCCTCAGACCGAGCT
probe-miR-424
miR-424
TTCAAAACATGAATTGCTGCTG
probe-miR-425
miR-425
GGCGGACACGACATTCCCGAT
probe-miR-425-
miR-425-
TCAACGGGAGTGATCGTGTCATT
5p
5p
probe-miR-429
miR-429
ACGGTTTTACCAGACAGTATTA
probe-miR-431
miR-431
TGCATGACGGCCTGCAAGACA
probe-miR-432
miR-432
CCACCCAATGACCTACTCCAAGA
nrobe-miR-432*
miR-432*
AGACATGGAGGAGCCATCCAG
probe-miR-433
miR-433
ACACCGAGGAGCCCATCATGAT
probe-miR-448
miR-448
ATGGGACATCCTACATATGCAA
probe-miR-449
miR-449
ACCAGCTAACAATACACTGCCA
probe-miR-450
miR-450
TATTAGGAACACATCGCAAAAA
probe-miR-451
miR-451
AAACTCAGTAATGGTAACGGTTT
probe-miR-452
miR-452
GTCTCAGTTTCCTCTGCAAACA
probe-miR-452*
miR-452*
CTTCTTTGCAGATGAGACTGA
probe-miR-453
miR-453
CGAACTCACCACGGACAACCTC
probe-miR-455
miR-455
CGATGTAGTCCAAAGGCACATA
probe-miR-483
miR-483
AGAAGACGGGAGGAGAGGAGTGA
probe-miR-484
miR-484
ATCGGGAGGGGACTGAGCCTGA
probe-miR-485-
miR-485-
AGAGGAGAGCCGTGTATGAC
3p
3p
probe-miR-485-
miR-485-
GAATTCATCACGGCCAGCCTCT
5p
5p
probe-miR-486
miR-486
CTCGGGGCAGCTCAGTACAGGA
probe-miR-487a
miR-487a
AACTGGATGTCCCTGTATGATT
probe-miR-487b
miR-487b
AAGTGGATGACCCTGTACGATT
probe-miR-488
miR-488
TTGAGAGTGCCATTATCTGGG
probe-miR-489
miR-489
GCTGCCGTATATGTGATGTCACT
probe-miR-490
miR-490
CAGCATGGAGTCCTCCAGGTTG
probe-miR-491
miR-491
TCCTCATGGAAGGGTTCCCCACT
probe-miR-492
miR-492
AAGAATCTTGTCCCGCAGGTCCT
probe-miR-493
miR-493
AATGAAAGCCTACCATGTACAA
probe-miR-493-
miR-493-
CTGGCACACAGTAGACCTTCA
3p
3p
probe-miR-494
miR-494
AAGAGGTTTCCCGTGTATGTTTCA
probe-miR-495
miR-495
AAAGAAGTGCACCATGTTTGTTT
probe-miR-496
miR-496
GAGATTGGCCATGTAAT
probe-miR-497
miR-497
ACAAACCACAGTGTGCTGCTG
probe-miR-498
miR-498
GAAAAACGCCCCCTGGCTTGAAA
probe-miR-499
miR-499
TTAAACATCACTGCAAGTCTTAA
probe-miR-500
miR-500
CAGAATCCTTGCCCAGGTGCAT
probe-miR-501
miR-501
TCTCACCCAGGGACAAAGGATT
probe-miR-502
miR-502
TAGCACCCAGATAGCAAGGAT
probe-miR-503
miR-503
CTGCAGAACTGTTCCCGCTGCTA
probe-miR-504
miR-504
ATAGAGTGCAGACCAGGGTCT
probe-miR-505
miR-505
GAGGAAACCAGCAAGTGTTGAC
probe-miR-506
miR-506
TCTACTCAGAAGGGTGCCTTA
probe-miR-507
miR-507
TTCACTCCAAAAGGTGCAAAA
probe-miR-508
miR-508
TCTACTCCAAAAGGCTACAATCA
probe-miR-509
miR-509
TCTACCCACAGACGTACCAATCA
probe-miR-510
miR-510
TGTGATTGCCACTCTCCTGAGTA
probe-miR-511
miR-511
TGACTGCAGAGCAAAAGACAC
probe-miR-512-
miR-512-
GACCTCAGCTATGACAGCACTT
3p
3p
probe-miR-512-
miR-512-
GAAAGTGCCCTCAAGGCTGAGTG
5p
5p
probe-miR-513
miR-513
ATAAATGACACCTCCCTGTGAA
probe-miR-514
miR-514
CTACTCACAGAAGTGTCAAT
probe-miR-515-
miR-515-
ACGCTCCAAAAGAAGGCACTC
3p
3p
probe-miR-515-
miR-515-
CAGAAAGTGCTTTCTTTTGGAGAA
5p
5p
probe-miR-516-
miR-516-
ACCCTCTGAAAGGAAGCA
3p
3p
probe-miR-516-
miR-516-
AAAGTGCTTCTTACCTCCAGAT
5p
5p
probe-miR-517*
miR-517*
AGACAGTGCTTCCATCTAGAGG
probe-miR-517a
miR-517a
AACACTCTAAAGGGATGCACGAT
probe-miR-517b
miR-517b
AACACTCTAAAGGGATGCACGA
probe-miR-517c
miR-517c
ACACTCTAAAAGGATGCACGAT
probe-miR-518a
miR-518a
TCCAGCAAAGGGAAGCGCTTT
probe-miR-
miR-518a-
AAAGGGCTTCCCTTTGCAGA
518a-2*
2*
probe-miR-518b
miR-518b
ACCTCTAAAGGGGAGCGCTTTG
probe-miR-518c
miR-518c
CACTCTAAAGAGAAGCGCTTTG
probe-miR-
miR-518c*
CAGAAAGTGCTTCCCTCCAGAGA
518c*
probe-miR-518d
miR-518d
GCTCCAAAGGGAAGCGCTTTG
probe-miR-518e
miR-518e
ACACTCTGAAGGGAAGCGCTTT
probe-miR-518f
miR-518f
TCCTCTAAAGAGAAGCGCTTT
probe-miR-
miR-518f*
AGAGAAAGTGCTTCCCTCTAGAG
518f*
probe-miR-519a
miR-519a
GTAACACTCTAAAAGGATGCACTTT
probe-miR-519b
miR-519b
AAACCTCTAAAAGGATGCACTTT
probe-miR-519c
miR-519c
ATCCTCTAAAAAGATGCACTTT
probe-miR-519d
miR-519d
ACACTCTAAAGGGAGGCACTTTG
probe-miR-519e
miR-519e
ACACTCTAAAAGGAGGCACTTT
probe-miR-
miR-519e*
GAAAGTGCTCCCTTTTGGAGAA
519e*
probe-miR-520a
miR-520a
ACAGTCCAAAGGGAAGCACTTT
probe-miR-
miR-520a*
AGAAAGTACTTCCCTCTGGAG
520a*
probe-miR-520b
miR-520b
CCCTCTAAAAGGAAGCACTTT
probe-miR-520c
miR-520c
AACCCTCTAAAAGGAAGCACTTT
probe-miR-520d
miR-520d
AACCCACCAAAGAGAAGCACTTT
probe-miR-
miR-520d*
CAGAAAGGGCTTCCCTTTGTAGA
520d*
probe-miR-520e
miR-520e
CCCTCAAAAAGGAAGCACTTT
probe-miR-520f
miR-520f
AACCCTCTAAAAGGAAGCACTT
probe-miR-520g
miR-520g
ACACTCTAAAGGGAAGCACTTTGT
probe-miR-520h
miR-520h
ACTCTAAAGGGAAGCACTTTGT
probe-miR-521
miR-521
ACACTCTAAAGGGAAGTGCGTT
probe-miR-522
miR-522
AACACTCTAAAGGGAACCATTTT
probe-miR-523
miR-523
CCCTCTATAGGGAAGCGCGTT
probe-miR-524
miR-524
ACTCCAAAGGGAAGCGCCTTC
probe-miR-524*
miR-524*
GAGAAAGTGCTTCCCTTTGTAG
probe-miR-525
miR-525
AGAAAGTGCATCCCTCTGGAG
probe-miR-525*
miR-525*
GCTCTAAAGGGAAGCGCCTTC
probe-miR-526a
miR-526a
AGAAAGTGCTTCCCTCTAGAG
probe-miR-526b
miR-526b
AACAGAAAGTGCTTCCCTCAAGAG
probe-miR-
miR-526b*
GCCTCTAAAAGGAAGCACTTT
526b*
probe-miR-526c
miR-526c
AACAGAAAGCGCTTCCCTCTAGAG
probe-miR-527
miR-527
AGAAAGGGCTTCCCTTTGCAG
probe-miR-532
miR-532
ACGGTCCTACACTCAAGGCATG
probe-miR-542-
miR-542-
TTTCAGTTATCAATCTGTCACA
3p
3p
probe-miR-542-
miR-542-
CTCGTGACATGATGATCCCCGA
5p
5p
probe-miR-544
miR-544
ACTTGCTAAAAATGCAGAAT
probe-miR-545
miR-545
CACACAATAAATGTTTGCTGAT
probe-miR-548a
miR-548a
GCAAAAGTAATTGCCAGTTTTG
probe-miR-548b
miR-548b
ACAAAAGCAACTGAGGTTCTTG
probe-miR-548c
miR-548c
GCAAAAGTAATTGAGATTTTTG
probe-miR-548d
miR-548d
GCAAAAGAAACTGTGGTTTTTG
probe-miR-549
miR-549
AGAGCTCATCCATAGTTGTCA
probe-miR-550
miR-550
ATGTGCCTGAGGGAGTAAGACA
probe-miR-551a
miR-551a
TGGAAACCAAGAGTGGGTCGC
probe-miR-552
miR-552
TTGTCTAACCAGTCACCTGTT
probe-miR-553
miR-553
AAAACAAAATCTCACCGTTTT
probe-miR-554
miR-554
ACTGGCTGAGTCAGGACTAGC
probe-miR-555
miR-555
ATCAGAGGTTCAGCTTACCCT
probe-miR-556
miR-556
CATATTACAATGAGCTCATC
probe-miR-557
miR-557
AGACAAGGCCCACCCGTGCAAAC
probe-miR-558
miR-558
ATTTTGGTACAGCAGCTCA
probe-miR-559
miR-559
TTTTGGTGCATATTTACTTTA
probe-miR-560
miR-560
GGCGGCCGGCCGGCGCACGC
probe-miR-561
miR-561
ACTTCAAGGATCTTAAACTTTG
probe-miR-562
miR-562
GCAAATGGTACAGCTACTTT
probe-miR-563
miR-563
GGGAAACGTATGTCAACCT
probe-miR-564
miR-564
GCCTGCTGACACCGTGCCT
probe-miR-565
miR-565
AAACAGACATCGCGAGCCAGCC
probe-miR-566
miR-566
GTTGGGATCACAGGCGCCC
probe-miR-567
miR-567
GTTCTGTCCTGGAAGAACATACT
probe-miR-568
miR-568
GTGTGTATACATTTATACAT
probe-miR-569
miR-569
ACTTTCCAGGATTCATTAACT
probe-miR-570
miR-570
TGCAAAGGTAATTGCTGTTTTC
probe-miR-571
miR-571
CTCACTCAGATGGCCAACTCA
probe-miR-572
miR-572
TGGGCCACCGCCGAGCGGAC
probe-miR-573
miR-573
CTGATCAGTTACACATCACTTCAG
probe-miR-574
miR-574
GTGGGTGTGTGCATGAGCGTG
probe-miR-575
miR-575
GCTCCTGTCCAACTGGCTC
probe-miR-576
miR-576
CAAAGACGTGGAGAAATTAGAAT
probe-miR-577
miR-577
CAGGTACCAATATTTTATCTA
probe-miR-578
miR-578
ACAATCCTAGAGCACAAGAAG
probe-miR-579
miR-579
ATCGCGGTTTATACCAAATGAAT
probe-miR-580
miR-580
CCTAATGATTCATCATTCTCAA
probe-miR-581
miR-581
ACTGATCTAGAGAACACAAGA
probe-miR-582
miR-582
AGTAACTGGTTGAACAACTGTAA
probe-miR-583
miR-583
GTAATGGGACCTTCCTCTTTG
probe-miR-584
miR-584
CTCAGTCCCAGGCAAACCATAA
probe-miR-585
miR-585
TAGCATACAGATACGCCCA
probe-miR-586
miR-586
GGACCTAAAAATACAATGCATA
probe-miR-587
miR-587
GTGACTCATCACCTATGGAAA
probe-miR-588
miR-588
GTTCTAACCCATTGTGGCCAA
probe-miR-589
miR-589
TCTGGGAACCGGCATTTGTTCTGA
probe-miR-590
miR-590
CTGCACTTTTATGAATAAGCTC
probe-miR-591
miR-591
ACAATGAGAACCCATGGTCT
probe-miR-592
miR-592
ACATCATCGCATATTGACACAA
probe-miR-593
miR-593
GCTGAGCAATGCCTGGCTGGTGCCT
probe-miR-594
miR-594
AAAGTCACAGGCCACCCCAGATGGG
probe-miR-595
miR-595
AGACACACCACGGCACACTTC
probe-miR-596
miR-596
CCCGAGGAGCCGGGCAGGCTT
probe-miR-597
miR-597
ACAGTGGTCATCGAGTGACACA
probe-miR-598
miR-598
TGACGATGACAACGATGACGTA
probe-miR-599
miR-599
GTTTGATAAACTGACACAAC
probe-miR-600
miR-600
GAGCAAGGCTCTTGTCTGTAAGT
probe-miR-601
miR-601
CTCCTCCAACAATCCTAGACCA
probe-miR-602
miR-602
GGGCCGCAGCTGTCGCCCGTGTC
probe-miR-603
miR-603
GCAAAAGTAATTGCAGTGTGTG
probe-miR-604
miR-604
GTCCTGAATTCCGCAGCCT
probe-miR-605
miR-605
AGGAGAAGGCACCATGGGATTTA
probe-miR-606
miR-606
ATCTTTGATTTTCAGTAGTTT
probe-miR-607
miR-607
GTTATAGATCTGGATTTGAAC
probe-miR-608
miR-608
ACGGAGCTGTCCCAACACCACCCCT
probe-miR-609
miR-609
AGAGATGAGAGAAACACCCT
probe-miR-610
miR-610
TCCCAGCACACATTTAGCTCA
probe-miR-611
miR-611
GTCAGACCCCGAGGGGTCCTCGC
probe-miR-612
miR-612
AAGGAGCTCAGAAGCCCTGCCCAGC
probe-miR-613
miR-613
GGCAAAGAAGGAACATTCCT
probe-miR-614
miR-614
CCACCTGGCAAGAACAGGCGTTC
probe-miR-615
miR-615
AGAGGGAGACCCAGGCTCGGA
probe-miR-616
miR-616
AAGTCACTGAAGGGTTTTGAGT
probe-miR-617
miR-617
GCCACCTTCAAATGGGAAGTCT
probe-miR-618
miR-618
ACTCAGAAGGACAAGTAGAGTTT
probe-miR-619
miR-619
ACTGGGCACAAACATGTCCAGGTC
probe-miR-620
miR-620
ATTTCTATATCTATCTCCAT
probe-miR-621
miR-621
AGGTAAGCGCTGTTGCTAGCC
probe-miR-622
miR-622
GCTCCAACCTCAGCAGACTGT
probe-miR-623
miR-623
ACCCAACAGCCCCTGCAAGGGAT
probe-miR-624
miR-624
TGAACACAAGGTACTGGTACTA
probe-miR-625
miR-625
AGGACTATAGAACTTTCCCCCT
probe-miR-626
miR-626
AAGACATTTTCAGACAGCT
probe-miR-627
miR-627
TCCTCTTTTCTTAGAGACTCAC
probe-miR-628
miR-628
CGACTGCCACTCTTACTAGA
probe-miR-629
miR-629
GCTGGGCTTACGTTGGGAGAAC
probe-miR-630
miR-630
ACCTTCCCTGGTACAGAATACT
probe-miR-631
miR-631
GCTGAGGTCTGGGCCAGGTCT
probe-miR-632
miR-632
TCCCACAGGAAGCAGACAC
probe-miR-633
miR-633
TTTATTGTGGTAGATACTATTAG
probe-miR-634
miR-634
GTCCAAAGTTGGGGTGCTGGTT
probe-miR-635
miR-635
GGACATTGTTTCAGTGCCCAAGT
probe-miR-636
miR-636
CTGCGGGCGGGACGAGCAAGCACA
probe-miR-637
miR-637
ACGCAGAGCCCGAAAGCCCCCAGT
probe-miR-638
miR-638
AGGCCGCCACCCGCCCGCGATCCCT
probe-miR-639
miR-639
ACAGCGCTCGCAACCGCAGCGAT
probe-miR-640
miR-640
AGAGGCAGGTTCCTGGATCAT
probe-miR-641
miR-641
GAGGTGACTCTATCCTATGTCTTT
probe-miR-642
miR-642
CAAGACACATTTGGAGAGGGAC
probe-miR-643
miR-643
CTACCTGAGCTAGCATACAAGT
probe-miR-644
miR-644
GCTCTAAGAAAGCCACACT
probe-miR-645
miR-645
TCAGCAGTACCAGCCTAGA
probe-miR-646
miR-646
GCCTCAGAGGCAGCTGCTT
probe-miR-647
miR-647
GAAGGAAGTGAGTGCAGCCAC
probe-miR-648
miR-648
ACCAGTGCCCTGCACACTT
probe-miR-649
miR-649
GACTCTTGAACAACACAGGTTT
probe-miR-650
miR-650
GTCCTGAGAGCGCTGCCTCCT
probe-miR-651
miR-651
CAAAAGTCAAGCTTATCCTAAA
probe-miR-652
miR-652
TGCACAACCCTAGTGGCGCCATT
probe-miR-653
miR-653
GTTCAGTAGAGATTGTTTCAA
probe-miR-654
miR-654
GCACATGTTCTGCGGCCCACCA
probe-miR-655
miR-655
AAAGAGGTTAACCATGTATTAT
probe-miR-656
miR-656
AGAGGTTGACTGTATAATATT
probe-miR-657
miR-657
CCTAGAGAGGGTGAGAACCTGCC
probe-miR-658
miR-658
ACCAACGGACCTACTTCCCTCCGCC
probe-miR-659
miR-659
TGGGGACCCTCCCTGAACCAAG
probe-miR-660
miR-660
CAACTCCGATATGCAATGGGTA
probe-miR-661
miR-661
ACGCGCAGGCCAGAGACCCAGGCA
probe-miR-662
miR-662
CTGCTGGGCCACAACGTGGGA
probe-miR-663
miR-663
GCGGTCCCGCGGCGCCCCGCCT
probe-miR-7
miR-7
CAACAAAATCACTAGTCTTCCA
probe-miR-9
miR-9
TCATACAGCTAGATAACCAAAGA
probe-miR-9*
miR-9*
ACTTTCGGTTATCTAGCTTTA
probe-miR-92
miR-92
CAGGCCGGGACAAGTGCAATA
probe-miR-93
miR-93
CTACCTGCACGAACAGCACTTT
probe-miR-95
miR-95
TGCTCAATAAATACCCGTTGAA
probe-miR-96
miR-96
GCAAAAATGTGCTAGTGCCAAA
probe-miR-98
miR-98
AACAATACAACTTACTACCTCA
probe-miR-99a
miR-99a
CACAAGATCGGATCTACGGGTT
probe-miR-99b
miR-99b
CGCAAGGTCGGTTCTACGGGTG
43 . The biochip according to claim 40 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to determine the physiological and/or pathological condition of the subject after being administrated a test sample.
44 . The biochip according to claims 43 , characterized in that the biochip are useful for screening the test sample for the activities on the prevention and/or treatment of diseases.
45 . The biochip according to claim 40 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to diagnose and/or differentially diagnose the diseases of the subject.
46 . The biochip according to claim 40 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to evaluate the effectiveness of treating the diseases of the subject.
47 . The biochip according to claim 40 , characterized in that said evaluating the physiological and/or pathological condition of a subject is to predict the occurrence of diseases of the subject.
48 . The biochip according to claim 47 , characterized in that the occurrence of diseases is the occurrence of complications and/or the relapse of malignant diseases.
49 . The biochip according to claim 44 , characterized in that the diseases include various tumors, various acute and chronic infectious diseases e.g. viral diseases such as viral influenza, viral hepatitis, AIDS, SARS, bacterial diseases such as tuberculosis, bacterial pneumonia, and other acute and chronic infectious diseases caused by various pathogenic microorganisms; other acute and chronic diseases such as diseases of respiratory system, diseases of immune system, diseases of blood and hematopoietic system, diseases of circulatory system such as cardio-cerebrovascular disease, metabolic diseases of endocrine system, diseases of digestive system, diseases of nervous system, diseases of urinary system, diseases of reproductive system and diseases of locomotor system.
50 . The biochip according to claim 40 , characterized, in that the biochip are useful for detecting the subject for prohibited drugs-taking.
51 . The chip according to claim 40 , characterized in that the serum/plasma the subject is from living bodies, tissues, organs and/or corpuses of the subject.Join the waitlist — get patent alerts
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