US2010184195A1PendingUtilityA1

Recycling System for Manipulation of Intracellular NADH Availability

50
Assignee: RICE UNIVERSITYPriority: Nov 2, 2001Filed: Feb 12, 2010Published: Jul 22, 2010
Est. expiryNov 2, 2021(expired)· nominal 20-yr term from priority
Inventors:Ka-Yiu SanSusana J. Berrios-RiveraGeorge N. Bennett
C12P 7/625C12P 1/00C12N 9/0036
50
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a vitamin, a pharmaceutical and a biodegraded organic molecule. The NADH recycling system effects metabolic flux of reductive pathways in aerobic and anaerobic environments.

Claims

exact text as granted — not AI-modified
1 - 10 . (canceled) 
     
     
         11 . A cell comprising a recombinant NADH-recycling system, wherein said system comprises a heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase. 
     
     
         12 . The cell of  claim 11 , wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a nucleotide sequence encoding an NAH+-dependent formate dehydrogenase. 
     
     
         13 . The cell of  claim 11 , wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a yeast nucleotide sequence encoding an NAH+-dependent dehydrogenase. 
     
     
         14 . The cell of  claim 11 , wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a yeast nucleotide sequence encoding an NAH+-dependent formate dehydrogenase. 
     
     
         15 . The cell of  claim 11 , wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a nucleotide sequence from  Candida boidinii.    
     
     
         16 . The cell of  claim 11 , wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a nucleotide sequence from  Candida boidinii  comprising SEQ ID NO: 1. 
     
     
         17 . The cell of  claim 11 , wherein said cell is a bacterium. 
     
     
         18 . The cell of  claim 11 , wherein said cell is  E. coli.    
     
     
         19 . The cell of  claim 11 , wherein said cell is a member of the genus  Rhodococcus.    
     
     
         20 - 47 . (canceled)

Join the waitlist — get patent alerts

Track US2010184195A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.